Competitive Adsorption of Interfacially Active Nanoparticles and Anionic Surfactant at the Crude Oil-Water Interface.

The interfacial activity of poly(N-isopropylacrylamide) (pNIPAM) nanoparticles in the absence and presence of an anionic surfactant (sodium dodecyl sulfate, SDS) was studied at a crude oil-water interface. Both species are interfacially active and can lower the interfacial tension, but when mixed together, the interfacial composition was found to depend on the aging time and total component concentration. With the total component concentration less than 0.005 wt %, the reduced interfacial tension by pNIPAM was greater than SDS; thus, pNIPAM has a greater affinity to partition at the crude oil-water interface. However, the lower molecular weight (smaller molecule) of SDS compared to pNIPAM meant that it rapidly partitioned at the oil-water interface. When mixed, the interfacial composition was more SDS-like for low total component concentrations (≤ 0.001 wt %), while above, the interfacial composition was more pNIPAM-like, similar to the single component response. Applying a weighted arithmetic mean approach, the surface-active contribution (%) could be approximated for each component, pNIPAM and SDS. Even though SDS rapidly partitioned at the oil-water interface, it was shown to be displaced by the pNIPAM nanoparticles, and for the highest total component concentration, pNIPAM nanoparticles were predominantly contributing to the reduced oil-water interfacial tension. These findings have implications for the design and performance of fluids that are used to enhance crude oil production from reservoirs, particularly highlighting the aging time and component concentration effects to modify interfacial tensions.

Dewetting dynamics of heavy crude oil droplet in low-salinity fluids at elevated pressures and temperatures.

HYPOTHESIS: Improved oil recovery by low-salinity injection correlates to the optimal brine concentration to achieve maximum dewetting of oil droplets on rock surfaces. While interfacial tension and electrical double layer forces are often cited as being determinant properties, we hypothesize that other structural/interfacial forces are more prominent in governing the system behavior. EXPERIMENTS: The sessile droplet technique was used to study the receding dynamics of oil droplets from flat hydrophilic substrates in brines of different salt type (NaCl and CaCl2) and concentration, and were studied at both low and elevated temperatures (60 and 140 °C) and pressures (1, 10, 100 and 200 bar). FINDINGS: At 1 bar and 60 °C, the minimum oil droplet-substrate adhesion force (FA) was determined at 34 mM NaCl and 225 mM CaCl2. For NaCl this strongly correlated to strengthening hydration forces, which for CaCl2 were diminished by long-range hydrophobic forces. These results highlight the importance of other non-DLVO forces governing the dewetting dynamics of heavy crude oil droplets. At 140 °C and 200 bar, the optimal brine concentrations were found to be much higher (1027 mM NaCl and 541 mM CaCl2), with higher concentrations likely attributed to weakening hydration forces at elevated temperatures.

Animal and Cellular Studies Demonstrate Some of the Beneficial Impacts of Herring Milt Hydrolysates on Obesity-Induced Glucose Intolerance and Inflammation.

The search for bioactive compounds from enzymatic hydrolysates has increased in the last few decades. Fish by-products have been shown to be rich in these valuable molecules; for instance, herring milt is a complex matrix composed of lipids, nucleotides, minerals, and proteins. However, limited information is available on the potential health benefits of this by-product. In this context, three industrial products containing herring milt hydrolysate (HMH) were tested in both animal and cellular models to measure their effects on obesity-related metabolic disorders. Male C57Bl/6J mice were fed either a control chow diet or a high-fat high-sucrose (HFHS) diet for 8 weeks and received either the vehicle (water) or one of the three HMH products (HMH1, HMH2, and HMH3) at a dose of 208.8 mg/kg (representing 1 g/day for a human) by daily oral gavage. The impact of HMH treatments on insulin and glucose tolerance, lipid homeostasis, liver gene expression, and the gut microbiota profile was studied. In parallel, the effects of HMH on glucose uptake and inflammation were studied in L6 myocytes and J774 macrophages, respectively. In vivo, daily treatment with HMH2 and HMH3 improved early time point glycemia during the oral glucose tolerance test (OGTT) induced by the HFHS diet, without changes in weight gain and insulin secretion. Interestingly, we also observed that HMH2 consumption partially prevented a lower abundance of Lactobacillus species in the gut microbiota of HFHS diet-fed animals. In addition to this, modulations of gene expression in the liver, such as the upregulation of sucrose nonfermenting AMPK-related kinase (SNARK), were reported for the first time in mice treated with HMH products. While HMH2 and HMH3 inhibited inducible nitric oxide synthase (iNOS) induction in J774 macrophages, glucose uptake was not modified in L6 muscle cells. These results indicate that milt herring hydrolysates reduce some metabolic and inflammatory alterations in cellular and animal models, suggesting a possible novel marine ingredient to help fight against obesity-related immunometabolic disorders.

Preparation of Magnetic Carboxymethylchitosan Nanoparticles for Adsorption of Heavy Metal Ions.

The remediation of metal and heavy metal contaminants from water ecosystems is a long-standing problem in the field of water management. The development of efficient, cost effective, and environmentally friendly natural polymer-based adsorbents is reported here. Magnetic chitosan (CS) and carboxymethylchitosan (CMC) nanocomposites have been synthesized by a simple one-step chemical coprecipitation method. The nanoparticles were assessed for the removal of Pb2+, Cu2+, and Zn2+ ions from aqueous solution. Kinetic and thermodynamic models were used to describe and understand the adsorption process of the ions onto the nanomaterials. The interactions between the ions and the biopolymer-based composites are reversible, which means that the nanoparticles can be regenerated in weakly acidic or EDTA containing solution without losing their activity and stability for water cleanup applications.

Erratum: Figure Correction: Probiotics as Complementary Treatment for Metabolic Disorders.

[This corrects the article on p. 291 in vol. 39, PMID: 26301190.].

Probiotics as Complementary Treatment for Metabolic Disorders.

Over the past decade, growing evidence has established the gut microbiota as one of the most important determinants of metabolic disorders such as obesity and type 2 diabetes. Indeed, obesogenic diet can drastically alter bacterial populations (i.e., dysbiosis) leading to activation of pro-inflammatory mechanisms and metabolic endotoxemia, therefore promoting insulin resistance and cardiometabolic disorders. To counteract these deleterious effects, probiotic strains have been developed with the aim of reshaping the microbiome to improve gut health. In this review, we focus on benefits of widely used probiotics describing their potential mechanisms of action, especially their ability to decrease metabolic endotoxemia by restoring the disrupted intestinal mucosal barrier. We also discuss the perspective of using new bacterial strains such as butyrate-producing bacteria and the mucolytic Akkermansia muciniphila, as well as the use of prebiotics to enhance the functionality of probiotics. Finally, this review introduces the notion of genetically engineered bacterial strains specifically developed to deliver anti-inflammatory molecules to the gut.

Liquid infused porous surfaces for mineral fouling mitigation.

Prevention of mineral fouling, known as scale, is a long-standing problem in a wide variety of industrial applications, such as oil production, water treatment, and many others. The build-up of inorganic scale such as calcium carbonate on surfaces and facilities is undesirable as it can result in safety risks and associated flow assurance issues. To date the overwhelming amount of research has mainly focused on chemical inhibition of scale bulk precipitation and little attention has been paid to deposition onto surfaces. The development of novel more environmentally-friendly strategies to control mineral fouling will most probably necessitate a multifunctional approach including surface engineering. In this study, we demonstrate that liquid infused porous surfaces provide an appealing strategy for surface modification to reduce mineral scale deposition. Microporous polypyrrole (PPy) coatings were fabricated onto stainless steel substrates by electrodeposition in potentiostatic mode. Subsequent infusion of low surface energy lubricants (fluorinated oil Fluorinert FC-70 and ionic liquid 1-Butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (BMIm)) into the porous coatings results in liquid-repellent slippery surfaces. To assess their ability to reduce surface scaling the coatings were subjected to a calcium carbonate scaling environment and the scale on the surface was quantified using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). PPy surfaces infused with BMIm (and Fluorinert to a lesser extent) exhibit remarkable antifouling properties with the calcium carbonate deposition reduced by 18 times in comparison to untreated stainless steel. These scaling tests suggest a correlation between the stability of the liquid infused surfaces in artificial brines and fouling reduction efficiency. The current work shows the great potential of such novel coatings for the management of mineral scale fouling.

Erratum: Figure Correction: Probiotics as Complementary Treatment for Metabolic Disorders

There was a spelling error in the Fig 2.

Probiotics as Complementary Treatment for Metabolic Disorders

Over the past decade, growing evidence has established the gut microbiota as one of the most important determinants of metabolic disorders such as obesity and type 2 diabetes. Indeed, obesogenic diet can drastically alter bacterial populations (i.e., dysbiosis) leading to activation of pro-inflammatory mechanisms and metabolic endotoxemia, therefore promoting insulin resistance and cardiometabolic disorders. To counteract these deleterious effects, probiotic strains have been developed with the aim of reshaping the microbiome to improve gut health. In this review, we focus on benefits of widely used probiotics describing their potential mechanisms of action, especially their ability to decrease metabolic endotoxemia by restoring the disrupted intestinal mucosal barrier. We also discuss the perspective of using new bacterial strains such as butyrate-producing bacteria and the mucolytic Akkermansia muciniphila, as well as the use of prebiotics to enhance the functionality of probiotics. Finally, this review introduces the notion of genetically engineered bacterial strains specifically developed to deliver anti-inflammatory molecules to the gut.

Development of anti-icing materials by chemical tailoring of hydrophobic textured metallic surfaces.

Ice on surfaces can have dramatic consequences for human activities. Over the last decades, the design of new materials with anti-icing properties has generated significant research efforts for the prevention of ice accretion. Here we investigate water freezing temperatures on untreated and negatively charged hydrophobic stainless steel surfaces and use these temperatures to evaluate icephobicity. Supercooled water microdroplets are deposited and undergo a slow controlled cooling until spontaneous freezing occurs. Textured hydrophobic stainless steel surfaces functionalized with anionic polyelectrolytes brushes display unexpectedly lower freezing temperatures, at least 7 °C lower than polished untreated steel. On the basis of the entropy reduction of the crystalline phase near a charged solid surface, we used a modification of the classical heterogeneous nucleation theory to explain the observed freezing temperatures lessening. Our results could help the design of new composite materials that more efficiently prevent ice formation.

Determining hepatitis C genotype by analyzing the sequence of the NS5b region.

Assays to determine the hepatitis C virus (HCV) genotype have recently become useful for clinical decision making and may be suitable for epidemiological investigations, such as identifying HCV outbreaks in a given population. Molecular assays are the most common diagnostic tools for HCV genotyping. This study compares two genome typing assays, one, the Trugene 5'NC genotyping kit, uses the sequence of the 5' non-coding (5'NC) region and the other, a non-commercial assay, uses the non-structural 5b (NS5b) region. Serum samples from 203 chronically HCV-infected patients were tested. The 5'NC and the NS5b assays were both very effective in identifying the genotype (99 and 98.5%) and the results with the two methods were always concordant for the genotype. The NS5b analysis permitted the identification of the subtype in all samples, whereas the 5'NC region assay did not in 33% of samples. The NS5b analysis showed that one patient had a mixed infection with HCV subtypes 1a and 2c, while the 5'NC assay did not. It is concluded that phylogenetic analysis using both the 5'NC and the NS5b regions are reliable and convenient methods for HCV typing in clinical practice. But analysis of the NS5b region may be more useful for tracing the source of an HCV infection.