Formation of Erysiphe necator Chasmothecia in the Pacific Northwest United States.

In the Pacific Northwest, chasmothecia formation is not observed in vineyards until the beginning of véraison despite heavy infestations whereby 100% of leaf tissue is covered by Erysiphe necator. Mating type proximity and distribution were sampled from individual lesions (∼71 mm2) on leaf tissue in a stratified sampling from three canopy heights at three times during the 2013, 2014, and 2015 growing seasons. Both mating types were observed at every sampling point and within the same lesions at all sampling dates and canopy heights. Effect of temperature and day length were examined by inoculating seedlings with known mating type 1 and 2 isolates and placed in incubators at different temperatures (5, 10, 15, 20, 25, and 30°C) or different day length changes (long day to long day, long day to short day, short day to short day, and short day to long day). Chasmothecia were produced at all temperatures that E. necator was able to colonize tissue, and the greatest number of chasmothecia were produced at 15 and 20°C (P ≤ 0.02). Day length shifts from short day (8 h) to long day (16 h) resulted in a significant increase in chasmothecia production (P < 0.001). End of season plant stress observed in the Pacific Northwest, such as water stress or host senescence, was assessed under naturally infested field conditions by either girdling canes or applying 150 mg·liter-1 abscisic acid solution to vines, respectively, and quantifying chasmothecia production. No differences were observed in chasmothecia production in the plant stress assessment, likely due to the high vigor and ability for plants to overcome stress treatments.

Assessment of Erysiphe necator Ascospore Release Models for Use in the Mediterranean Climate of Western Oregon.

Predictive models have been developed in several major grape-growing regions to correlate environmental conditions to Erysiphe necator ascospore release; however, these models may not be broadly applicable in regions with different climatic conditions. To assess ascospore release in near-coastal regions of western Oregon, chasmothecia (syn. cleistothecia) were collected prior to leaf drop and placed onto natural and artificial grape trunk segments and overwintered outside. Ascospore release was monitored for three overwintering seasons using custom impaction spore traps from leaf drop (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie [BBCH] 97) until the onset of the disease epidemic in the following growing season. Airborne inoculum was concurrently monitored in a naturally infested research vineyard. Weather and ascospore release data were used to assess previously developed models and correlate environmental conditions to ascospore release. Ascospore release was predicted by all models prior to bud break (BBCH 08), and was observed from the first rain event following the start of inoculum monitoring until monitoring ceased. Previously developed models overpredicted ascospore release in the Willamette Valley and predicted exhaustion of inoculum prior to bud break. The magnitude of ascospore release could not be correlated to environmental conditions; thus, a binary ascospore release model was developed where release is a function of the collective occurrence of the following factors within a 24-h period: >6 h of cumulative leaf wetness during temperatures >4°C, precipitation >2.5 mm, and relative humidity >80%. The Oregon model was validated using field-collected ascospore datasets, and predicted ascospore release with 66% accuracy (P = 0.02). Extant methods for estimating ascospore release may not be sufficiently accurate to use as predictive models in wet, temperate climatic regions.

Timing Fungicide Application Intervals Based on Airborne Erysiphe necator Concentrations.

Management of grape powdery mildew (Erysiphe necator) and other polycyclic diseases relies on numerous fungicide applications that follow calendar or model-based application intervals, both of which assume that inoculum is always present. Quantitative molecular assays have been previously developed to initiate fungicide applications, and could be used to optimize fungicide application intervals throughout the growing season based on inoculum concentration. Airborne inoculum samplers were placed at one research and six commercial vineyards in the Willamette Valley of Oregon. Fungicide applications in all plots were initiated at the first detection of E. necator inoculum, and all subsequent fungicide application intervals were made based the grower' standard calendar program or based on inoculum concentration. In adjusted-interval plots, fungicides were applied at the shortest labeled application interval when >10 spores were detected and the longest labeled application interval when <10 spores were detected. Fungicide applications in control plots consisted of the growers' standard management practice. An average of 2.3 fewer fungicide applications in 2013 and 1.6 fewer fungicide applications in 2014 were used in the adjusted fungicide application intervals treatment in grower fields without significant differences in berry or leaf disease incidence between treatments.