Organoruthenium metallocycle induced mutation in gld-1 tumor suppression gene in JK1466 strain and appreciable lifespan expansion.

Four Ru(II) complexes (A2-A5) were synthesized from the reaction of coumarin Schiff base ligands (7da2-tsc, 7da3-mtsc, 7da4-etsc and 7da5-ptsc) with [RuHCl(CO)(PPh3)3]. The compounds were characterized by FT-IR, UV-Vis, 1H, 13C and 31P NMR, mass spectrometry and crystallographic analysis. Calf Thymus DNA (CT-DNA) binding studies revealed the intercalative mode of binding of the complexes with DNA. The results of Bovine serum albumin (BSA) binding studies established the interaction between BSA followed static quenching mechanism. The cytotoxic effects of the complexes and the ligands were evaluated against breast (MCF-7 and MDA-MB-231) and lung carcinoma cell lines (A549 and NCI-H460) using MTT assay. Complex A4 demonstrated potent cytotoxic effects on both breast and lung cancer cells. Furthermore, morphological observations and FACS analysis showed the decrease in cell density by complex A4 by induced morphological changes and apoptotic body formation and cell death in both breast and lung cancer cells. Moreover, the invertebrate model Caenorhabditis elegans was employed to assess the in vivo anticancer activity of compound A4. The findings indicated that the treatment with A4 reduced tumor development and significantly extended organismal lifespan by 64 % in the tumoral strain JK1466 without adversely affecting essential physiological functions of the worm. Additionally, A4 demonstrated an upregulation of two crucial antioxidant defense genes. Overall, these results suggested that the compound A4 can be a potential candidate with novel chemotherapeutic applications.

Effect of ruthenium(II) complexes on MDA-MB-231 cells and lifespan/tumor growth in gld-1mutant, Daf-16 TF and stress productive genes: A perspective study.

Pincer type coumarin based N-substituted semicarbazone ligands HL1-4 and their corresponding ruthenium(II) complexes (1-4) were synthesized, analyzed and confirmed by various spectro analytical techniques. The molecular structure of the ligand HL3 and complex 3 was confirmed by single crystal X-ray diffraction analysis. The stoichiometry of complexes 1, 2 and 4 was confirmed by high resolution mass spectroscopy (HRMS). The binding affinity of the compounds with CT-DNA (Calf Thymus DNA) and BSA (Bovine Serum Albumin) was established by absorption and emission titration methods. The results of In vitro cytotoxicity showed the significant cytotoxic potential of the complexes against MDA-MB-231 cells (TNBC- Triple-negative breast cancer). Among the complexes, 1 and 4 have shown appreciable results. Further, antimigratory activity against the MDA-MB-231 cells was studied for the complexes 1 and 4. The percentage cell cycle arrest, apoptosis and necrosis were explored by flow cytometry. The in vivo anti-tumor activity of the complexes 1 and 4 using C. elegans as model organism was established by using the tumoral C. elegans strain JK1466 (gld-1(q485)), which bears a mutation in the gld-1 tumor suppressor gene. We have determined the effect of our complexes on tumor gonad reduction and found to be non toxic to the JK1466 worms and they have prolonged their mean lifespan with potential antioxidant ability by overcoming stress responses. Overall, our study reported herein demonstrated that the complexes 1 and 4 could be established as potential metallo-drugs substantiating further exploration.

An aggregation-induced emission-based ratiometric fluorescent chemosensor for Hg(II) and its application in Caenorhabditis elegans imaging.

A chromone-based ratiometric fluorescent probe L2 was developed for the selective detection of Hg(II) in a semi-aqueous solution based on aggregation-induced emission (AIE) and chelation-enhanced fluorescence (CHEF) effect. The probe L2 fluoresced significantly at 498 nm in its aggregated state, and when chelated with Hg(II), the soluble state fluoresced 1-fold higher. In addition, Job's plot reveals that the probe forms a 1:1 stoichiometry complex with Hg(II) with an association constant of 9.10 × 103M-1 estimated by the BH plot. The probe L2 detects Hg(II) down to 22.47 nM without interference from other interfering ions. The FTIR, ESI mass, and DFT-based computational studies investigated the binding mechanism of probe L2 with Hg(II). Taking advantage of its AIE characteristics, the probe L2 was successfully applied for bio-capability analysis in Caenorhabditis elegans (a nematode worm) imaging of Hg(II) in a living model.

Engineering the surface of graphene oxide with bovine serum albumin for improved biocompatibility in Caenorhabditis elegans.

Graphene oxide (GO) has been extensively studied for its potential biomedical applications. However, its potential risk associated with the interactions of GO in a biological system hampers its biomedical applications. Therefore, there is an urgent need to enhance the biocompatibility of GO. In the present study, we decorated the surface of GO with bovine serum albumin (GO-BSA) to mitigate the in vivo toxic properties of GO. An in vivo model Caenorhabditis elegans has been used to study the potential protective effect of BSA decoration in mitigating GO induced toxicity. The BSA decoration on the surface of GO prevents the acute and prolonged toxicity induced by GO in primary and secondary organs by maintaining normal intestinal permeability, defecation behavior, development, and reproduction. Notably, GO-BSA treatment at 0.5-100 mg L-1 does not affect the intracellular redox status and lifespan of C. elegans. Reporter gene expression analysis revealed that exposure to GO-BSA (100 mg L-1) did not significantly influence the nuclear accumulation and expression patterns of DAF-16/FOXO and SKN-1/Nrf2 transcription factors and their downstream target genes sod-3, hsp-16.2, ctl-1,2,3, gcs-1, and gst-4 when compared to exposure to pristine GO. Also, quantitative real-time PCR results showed that GO-BSA did not alter the expression of genes involved in regulating DNA damage checkpoints (cep-1, hus-1 and egl-1) and core signaling pathways of apoptosis (ced-4, ced-3 and ced-9), in contrast to GO treatment. All these findings will have an impact on the future development of safer nanomaterial formulations of graphene and graphene-based materials for environmental and biomedical applications.

East Indian sandalwood (Santalum album L.) oil confers neuroprotection and geroprotection in Caenorhabditis elegans via activating SKN-1/Nrf2 signaling pathway.

East Indian Sandalwood Oil (EISO) has diverse beneficial effects and has been used for thousands of years in traditional folk-medicine for treatment of different human ailments. However, there has been no in-depth scientific investigation to decipher the neuroprotective and geroprotective mechanism of EISO and its principle components, α- and ß-santalol. Hence the current study was undertaken to assess the protective effects of EISO, and α- and ß-santalol against neurotoxic (6-OHDA/6-hydroxydopamine) and proteotoxic (α-synuclein) stresses in a Caenorhabditis elegans model. Initially, we found that EISO and its principle components exerted an excellent antioxidant and antiapoptotic activity as it was able to extend the lifespan, and inhibit the ROS generation, and germline cell apoptosis in 6-OHDA-intoxicated C. elegans. Further, we showed that supplementation of EISO, and α- and ß-santalol reduced the 6-OHDA and α-synuclein-induced Parkinson's disease associated pathologies and improved the physiological functions. The genetic and reporter gene expression analysis revealed that an EISO, or α- and ß-santalol-mediated protective effect does not appear to rely on DAF-2/DAF-16, but selectively regulates SKN-1 and its downstream targets involved in antioxidant defense and geroprotective processes. Together, our findings indicated that EISO and its principle components are worth exploring further as a candidate redox-based neuroprotectant for the prevention and management of age-related neurological disorders.

Structural and optical studies of pHEMA encapsulated ZnS:Ni²âº nanoparticles.

In this study, ZnS:Ni(2+) nanostructures have been synthesized through chemical precipitation method using poly (2-hydroxyethyl methacrylate) (pHEMA) as capping agent. The structural, morphological and optical properties at different pHEMA concentration of ZnS:Ni(2+) were studied by using X-ray diffraction (XRD), high resolution transmission electron microscopy (HR-TEM), UV-Vis Spectroscopy (UV-Vis), fourier transform infrared spectroscopy (FT-IR), Photoluminescence (PL). The Average crystalline size of the nanoparticles was found to be in the range of ∼3.59-4.36 nm. The surface morphological analysis reveals that the pHEMA capped nanoparticles showed homogeneous smooth surface. HR-TEM analysis reminds the original size of pHEMA capped nanoparticles. The band gap investigation revealed the size dependent of quantum confined nanoparticles. The immobilized nanoparticles in pHEMA matrix were verified by FT-IR studies. Novel luminescence properties have been observed for uncapped and pHEMA capped ZnS nanoparticles. The optimal capping concentration was successfully determined and its influence on photoluminescence behavior has been thoroughly analyzed.

FT-IR spectroscopic, thermal analysis of human urinary stones and their characterization.

In the present study, FT-IR, XRD, TGA-DTA spectral methods have been used to investigate the chemical compositions of urinary calculi. Multi-components of urinary calculi such as calcium oxalate, hydroxyl apatite, struvite and uric acid have been studied. The chemical compounds are identified by FT-IR spectroscopic technique. The mineral identification was confirmed by powder X-ray diffraction patterns as compared with JCPDS reported values. Thermal analysis techniques are considered the best techniques for the characterization and detection of endothermic and exothermic behaviors of the urinary stones. The percentages of each hydrate (COM and COD) are present together, in the presences of MAPH or UA. Finally, the present study suggests that the Urolithiasis is significant health problem in children, and is very common in some parts of the world, especially in India. So that present study is so useful and helpful to the scientific community for identification of latest human health problems and their remedies using spectroscopic techniques.

Chemical composition and binary mixture of human urinary stones using FT-Raman spectroscopy method.

In the present study the human urinary stones were observed in their different chemical compositions of calcium oxalate monohydrate, calcium oxalate dihydrate, calcium phosphate, struvite (magnesium ammonium phosphate), uric acid, cystine, oxammite (ammonium oxalate monohydrate), natroxalate (sodium oxalate), glushinkite (magnesium oxalate dihydrate) and moolooite (copper oxalate) were analyzed using Fourier Transform-Raman (FT-Raman) spectroscopy. For the quantitative analysis, various human urinary stone samples are used for ratios calculation of binary mixtures compositions such as COM/COD, HAP/COD, HAP/COD, Uric acid/COM, uric acid/COD and uric acid/HAP. The calibration curve is used for further analysis of binary mixture of human urinary stones. For the binary mixture calculation the various intensities bands at 1462 cm(-1) (I(COM)), 1473 cm(-1) (I(COD)), 961 cm(-1) (I(HAP)) and 1282 cm(-1) (I(UA)) were used.

FT-Raman spectral analysis of human urinary stones.

FT-Raman spectroscopy is the most useful tool for the purpose of bio-medical diagnostics. In the present study, FT-Raman spectral method is used to investigate the chemical composition of urinary calculi. Urinary calculi multi-components such as calcium oxalate, hydroxyl apatite, struvite and uric acid are studied. FT-Raman spectrum has been recorded in the range of 3500-400 cm(-1). Chemical compounds are identified by Raman spectroscopic technique. The quantitative estimations of calcium oxalate monohydrate (COM) 1463 cm(-1), calcium oxalate dehydrate (COD) 1478 cm(-1), hydroxyl apatite 959 cm(-1), struvite 575 cm(-1), uric acid 1283 cm(-1) and oxammite (ammonium oxalate monohydrate) 2129 cm(-1) are calculated using particular peaks of FT-Raman spectrum. The quantitative estimation of human urinary stones suitable for the single calibration curve was performed.

FT-IR spectral studies on certain human urinary stones in the patients of rural area.

Fourier transform infrared spectroscopy (FT-IR) has been carried out to analyze the organic and inorganic constituent of human urinary stones. Patient's hailing from Rajah Muthiah Medical College and Hospital, Annamalai University, Tamil Nadu, India was selected for the study. The FT-IR results indicate that stones have different composition, i.e., namely calcium oxalate, calcium phosphate, carbonate apatite and magnesium ammonium phosphate and uric acid. From the spectral and powder X-ray diffraction pattern, the chemical constituents of urinary stones were identified. The quantitative estimations of calcium oxalate monohydrate (COM) 1,620 cm(-1), calcium phosphate (apatite) 1,037 cm(-1), magnesium ammonium phosphate (struvite) 1,010 cm(-1), calcium carbonate 1,460 cm(-1) and uric acid 1,441 cm(-1) were calculated using particular peaks of FT-IR studies. The study reveals that calcium oxalate monohydrate and calcium phosphate type urinary stones were predominant whereas magnesium ammonium phosphate are in moderate level, and calcium carbonate and uric acid are in low. Calcium phosphate is found in all the stones and calcium oxalate monohydrate is found to be higher. Quantitative analyses of urinary stones show that calcium oxalate monohydrate (40%), apatite (30%), magnesium ammonium phosphate (23%) and uric acid (7%) are present in all the urinary stone samples.