Structural and thermodynamic insights into the Cren7 mediated DNA organization in Crenarchaeota.

Archaea have histone homologues and chromatin proteins to organize their DNA into a compact form. This allows them to survive in extreme climates. Cren7 is one such chromatin protein conserved in Crenarchaeota. When Cren7 binds to model natural DNA, calf thymus DNA (CTD, 58% AT content) and polynucleotides under adverse solution conditions (high temperature, ionic strength), CD bands at 275-290 nm shift to higher wavelengths indicating structural changes in DNA. It formed a strong complex with CTD and poly(dA-dT)·poly(dA-dT), via a combination of electrostatic and non-electrostatic interactions. A low binding enthalpy indicated that the process was driven by entropy. The interaction was independent of the nature of the anions present in the solution. On studying the variation in protein affinity with salt concentration, it was estimated that the electrostatic interaction at the interface involves 3 pairs of ions at the protein-DNA interface. The affinity and binding site size decreased on changing the pH of the solution (between pH 6 and 8), but temperature did not result in such effects. Cren7 bound to 10 bp of DNA, increasing its flexibility and thermal stability by more than 30 °C. Increasing the amount of Cren7 produces cooperative structural transitions in DNAs without any similar transition in the protein. These crucial binding parameters, energetics, and structural changes decipher the mystery of Cren7 mediated DNA organization in Crenarchaeota.

Protein fibril assisted chiral assembly of gold nanorods.

Template mediated assembly of plasmonic nanomaterials is a promising approach to induce chirality. Naturally occurring macromolecules can self-assemble to form chiral superstructures, with dimensions extending from nanometer to micrometer length scales. These structures can serve as templates for host plasmonic nanomaterials on their surface through a variety of interactions. The arrangement of nanomaterials on these structures results in a transfer of symmetry from these templates to nanomaterials, which finally generates a chiral response in circular dichroism (CD) spectroscopy. For biosensing and in vitro applications of chiral plasmonics, long-term stability of these templates will be crucial for this approach of chirality induction. Here, we have demonstrated how protein amyloid fibrils can be used as templates to generate a chiroptical response with plasmonic nanomaterials. The temperature and ionic strength of the solution were carefully altered to convert the three-dimensional protein structure into amyloid fibrils. Changes in solution conditions affected the amyloid geometry, long-term stability, and interaction with AuNRs. The modified interactions influenced the orientation of the AuNRs, which affected the intensity of the CD response. The MTT assay indicated that the chiral AuNRs exhibited considerable cell viability, making them ideal for in vivo applications.