Tillage and manure effects on runoff nitrogen and phosphorus losses from frozen soils.

In cold regions, nutrient losses from dairy agroecosystems are a longstanding and recurring problem, especially when manure is applied during winter over snow-covered frozen soils. This study evaluated two tillage (fall chisel tillage [CT] and no-tillage [NT]) and three manure-type management treatments (unmanured control, liquid manure [<5% solids], and solid manure [>20% solids]). The liquid and solid manure used in this study were from the same animal species (Bos taurus) and facility. The six management treatments were field tested in south-central Wisconsin during the winters (November-April) of 2017-2018 and 2018-2019 with a complete factorial design. Seasonal runoff losses were significantly lower from fall CT compared with NT during both seasons. Manure applications (both liquid and solid) on top of snow significantly increased most of the nutrients (NH4 + , dissolved reactive phosphorus, total Kjeldahl nitrogen, and total phosphorus) in runoff compared with unmanured control. Irrespective of tillage and multiple runoff events, solid manure was present on the surface for longer periods, potentially releasing nutrients each time it interacted with runoff. In contrast, liquid manure infiltrated the snowpack and was partly lost with snowmelt and infiltrated soil depending upon soil frost and surface conditions. Overall, results indicate that wintertime manure applications over snow-covered frozen soils pose a risk of nutrient loss irrespective of tillage and manure type, but in unavoidable situations, prioritizing tillage × manure type combination can help reduce losses.

Predictive models of phosphorus concentration and load in stormwater runoff from small urban residential watersheds in fall season.

Urban street trees are a key part of public green infrastructure in many cities, however, leaf litter on streets is a critical biogenic source of phosphorus (P) in urban stormwater runoff during Fall. This study identified mass of street leaf litter (Mleaf) and antecedent dry days (ADD) as the top two explanatory parameters that have significant predictive power of event end-of-pipe P concentrations through multiple linear regression (MLR) analysis. Mleaf and volume of runoff (Vol) were the top two key explanatory parameters of event end-of-pipe P loads. Two-predictor MLR models were developed with these explanatory parameters using a 40-storm dataset derived from six small urban residential watersheds in Wisconsin, USA, and evaluated using storms specific to each study basin. The MLR model validation results indicated sensitivity to storm composition in the datasets. Our analysis shows selected parameters can be used by environmental managers to facilitate end-of-pipe P prediction in urban areas. This information can be used to reduce the amount of P in stormwater runoff by adjusting the timing and frequency of municipal leaf collection and street cleaning programs in urban areas.

Correlations between land use and stream nitrate-nitrite concentrations in the Yahara River Watershed in south-central Wisconsin.

To better inform land management decisions, we explored relationships between land use data and stream nitrate-nitrite (NO3NO2) concentration data in the Yahara River Watershed (YRW) in south-central Wisconsin, USA. Three metrics were used to evaluate the extent of different land uses in the watershed: (1) the area percentage of each land use in both the watershed and in a range of riparian zone widths, (2) the area factor, which refers to the ratio of the area of woodland, recreational, open and vacant subdivided land, or wetlands in the riparian zone (6.1-213.4 m widths) to agricultural areas in the rest of watershed, which indicates the buffering capacity of the riparian zone, and (3) the inverse-distance-weighted (IDW) area percentage with proximity to sub-watershed outlet and to stream, which characterizes spatial arrangement in the watershed by assigning a higher weight to patches closer to the outlet or stream and a lower weight to those farther away. We found significant, positive correlations between the extent of agricultural areas and stream NO3NO2 concentrations. NO3NO2 concentrations were highly correlated to area factor metrics for all riparian zone widths such that as area factor decreased, NO3NO2 concentrations increased. There was also a marked increase in NO3NO2 concentrations at a threshold of approximately 60% agricultural area with IDW proximity to stream. Wetland area percentage in the entire watershed and IDW wetland area percentage with proximity to stream were negatively correlated to stream NO3NO2 concentrations. Compared to the simple area percentage metric, area factor and IDW wetland area percentage with proximity to stream were better indicators of stream NO3NO2 concentrations. Results from this study indicate that, in addition to land use area percentage, spatial distributions of land uses should be considered when managing watersheds. This study also demonstrates the value of citizen-based sampling data and reveals opportunities to improve the utility of such data.

Novel determination of effective freeze-thaw cycles as drivers of ecosystem change.

Soil freeze-thaw cycles (FTCs) profoundly influence biophysical conditions and modify biogeochemical processes across many northern-hemisphere and alpine ecosystems. How FTCs will contribute to global processes in seasonally snow-covered ecosystems in the future is of particular importance as climate change progresses and winter snowpacks decline. Our understanding of these contributions is limited because there has been little consideration of inter- and intrayear variability in the characteristics of FTCs, in part due to a limited appreciation for which of these characteristics matters most with respect to a given biogeochemical process. Here, we introduce the concept of effective FTCs: those that are most likely linked to changes in key soil processes. We also propose a set of parameters to quantify and characterize effective FTCs using standard field soil temperature data. To put these proposed parameters into effective practice, we present FTCQuant, an R package of functions that quantifies FTCs based on a set of user-defined parameter criteria and, importantly, summarizes the individual characteristics of each FTC counted. To demonstrate the utility of these new concepts and tools, we applied the FTCQuant package to re-analyze data from two published studies to help explain over-winter changes to N2 O emissions and wet-aggregate stability. We found that effective FTCs would be defined differently for each of these response variables and that effective FTCs provided a 76 and 33% increase in model fit for wet-aggregate stability and cumulative N2 O emission, respectively, relative to conventional FTC quantification methods focusing on fluctuations around 0 °C. These results demonstrate the importance of identifying effective FTCs when scaling soil processes to regional or global levels. We hope our contributions will inform future deductions, hypothesis generation, and experimentation with respect to expected changes in freeze-thaw cycling globally.

Leachable phosphorus from senesced green ash and Norway maple leaves in urban watersheds.

In urban watersheds, street tree leaf litter is a critical biogenic source of phosphorus (P) in stormwater runoff. Stormwater extracts P from leaf litter and transports it, through the storm sewer network, to a receiving waterbody potentially causing downstream eutrophication. The goal of this study is to understand P leaching dynamics of two prevalent tree species (Norway maple (Acer platanoides) and green ash (Fraxinus pennsylvanica)) in three urban residential watersheds in Madison, Wisconsin, USA. Leaf litter was collected from the three basins during Fall 2017 and 2018. Laboratory experiments showed an initial rapid total dissolved phosphorus (TDP) release that gradually plateaued over a 48-hour period. The total TDP released from Norway maple (2.10 mg g-1) was greater than from green ash (1.60 mg g-1). Within the same species, increased fragmentation of leaves led to more rapid initial TDP release, but not greater total TDP release. Increased aging of senescent leaves decreased total TDP release. Incubation temperature and volume of water in contact with leaves may not be critical factors affecting TDP leaching dynamics. Predictive equations were derived to characterize time-variable TDP release of both Norway maple and green ash leaves. Potential TDP release from leaf litter estimated using these equations was compared with field-measured end-of-pipe TDP loads in one of the study watersheds. Our results indicate that preventing leaf litter from accumulating in streets is an important stormwater quality control measure.

Effects of Manure and Tillage on Edge-of-Field Phosphorus Loss in Seasonally Frozen Landscapes.

Environmental conditions and management practices affect nutrient losses in surface runoff, but their relative impacts on phosphorus (P) loss during frozen and nonfrozen ground periods have not been well quantified. More specifically, the relative importance of manure application, tillage, and soil-test P (STP) has not been assessed at the field scale. In this study, we compiled a dataset composed of 125 site-years of data from 26 fields that were continually monitored for edge-of-field P loss during snowmelt and storm events. Regression tree analyses were performed to rank the level of influence each environmental and management factor had on nutrient loads. Dissolved P (DP) was the majority of the total P (TP) during frozen conditions, but a small portion of TP during nonfrozen conditions. Manure application had a greater influence on the flow-weighted mean concentrations (FWMCs) of TP and DP during frozen conditions than during nonfrozen conditions. No-till resulted in greater TP and DP FWMCs during frozen conditions than conventional tillage, whereas the opposite effect for TP FWMC was seen during nonfrozen conditions. However, regression tree analysis revealed that STP (0- to 5-cm depth) was the most important factor in predicting DP and TP FWMCs during frozen conditions and DP FWMC during nonfrozen conditions. Extremely high STP values were associated with late-frozen manure applications and grazed pastures. Reducing surface P loss in seasonally frozen landscapes will require prioritizing management strategies that avoid manure application through early- and late-frozen conditions and lead to a drawdown of STP, particularly in the top 5 cm.

Preventing Pressure Injuries in Neonates Undergoing Therapeutic Hypothermia for Hypoxic-Ischemic Encephalopathy: An Interprofessional Quality Improvement Project.

BACKGROUND: Hospital-acquired pressure injuries (HAPIs) can be caused by multiple factors including pressure, shear, friction, moisture/incontinence, device-related pressure, immobility, inactivity, and nutritional deficits. Along with immobility, medical device-related (MDR) HAPIs are a primary cause of pressure injury in neonates, as the clinical practice setting has become increasingly technologically advanced. It is estimated that up to 50% of HAPIs are MDR in pediatric patients. Neonates are at particular risk for HAPI because of their specific anatomical, physiological, and developmental vulnerabilities. A specific example of confluent factors that may increase risk for HAPI is the application of therapeutic hypothermia (TH) and continuous electroencephalography monitoring for neonates with hypoxic-ischemic encephalopathy (HIE). INTERVENTIONS: An interprofessional team collaborated to expand upon existing evidence-based standards of care to address the needs of this specific population within the neonatal intensive care unit (NICU). Interventions centered on revision of current protocols, with efforts to optimize product selection, hardwire assessment practices, and refine documentation of patient care and outcomes. METHODS: The team primarily utilized plan-do-study-act (PDSA) cycles to test and refine specific methods and strategies to reduce HAPIs. Tested solutions were adopted, adapted, or abandoned. RESULTS: A sustained zero HAPI rate in the HIE population resulted. The team continues to collect, report, and utilize near-miss data to continue to refine the process as new risks are identified. IMPLICATIONS FOR PRACTICE: Recognizing the unique skin protection needs of special populations within the NICU, such as those undergoing TH, is crucial. When evidence-based standards of care fail to adequately meet such needs, a collaborative approach to identifying, testing, and implementing population-specific solutions is essential. IMPLICATIONS FOR RESEARCH: A paucity of literature regarding the unique skin protection needs for babies undergoing TH exists. Work should be done to better describe the influence of TH on skin integrity, with the goal of identifying population-specific protective measures.

Subsurface Transport of in Soils of Wisconsin's Carbonate Aquifer Region.

is a waterborne pathogen known to have a significant reservoir in bovine manure. Land-dependent manure disposal may not result in significant or reliable pathogen attenuation and, therefore, presents a risk for transport of pathogenic spp. to groundwater. One factor missing in the existing literature is the role soil characteristics play in affecting oocyst transport. Of specific concern in regions with carbonate geology are macropores and other soil structures that contribute to preferential flow. Therefore, research is needed to understand soil type effects and important transport pathways for pathogens such as oocysts to drinking water wells. This study investigated transport potential in several soils overlying Wisconsin's vulnerable carbonate aquifer and related the soil transport to soil series and textural class. Experimental work involved monitoring the transport of oocysts through intact soil columns of different soil series under simulated rain conditions. Results demonstrate that a significant portion of oocysts will sorb or be physically entrapped in the soil, especially in soil with high clay content. However, silt loam soils with comparatively lower clay content demonstrated an ability to transport oocysts through the soil profile primarily via the first flush of water infiltrating through soil macropores. The rate of oocyst migration in silt loam soils paralleled the bromide tracer front, thus bypassing the soils' ability to strain or adsorb oocysts out of infiltrating water. Nevertheless, proper manure treatment and management are necessary to minimize public health risks.

Concept mapping in a critical care orientation program: a pilot study to develop critical thinking and decision-making skills in novice nurses.

BACKGROUND: Newly graduated registered nurses who were hired into a critical care intensive care unit showed a lack of critical thinking skills to inform their clinical decision-making abilities. METHODS: This study evaluated the effectiveness of concept mapping as a teaching tool to improve critical thinking and clinical decision-making skills in novice nurses. A self-evaluation tool was administered before and after the learning intervention. RESULTS: The 25-item tool measured five key indicators of the development of critical thinking skills: problem recognition, clinical decision-making, prioritization, clinical implementation, and reflection. Statistically significant improvements were seen in 10 items encompassing all five indicators. CONCLUSION: Concept maps are an effective tool for educators to use in assisting novice nurses to develop their critical thinking and clinical decision-making skills.

Seizure detection using digital trend analysis: Factors affecting utility.

BACKGROUND: EEG monitoring is important for the early detection of seizures during the course of critical illness. However, the logistics of real time EEG interpretation is challenging for the neurophysiology and critical care medicine teams. This study evaluated factors affecting the utility of digital trend analysis (DTA) for rapid seizure identification in children. METHODS: digital EEG files of seizures in critically ill children were retrieved for DTA. The envelop trend (ET) and compressed spectral array (CSA) were applied to the raw EEG data and presented to an experienced and inexperienced user for interpretation who were blinded to conventional EEG findings. The EEG findings with and without presence of seizures and features of seizures were analyzed. RESULTS: we found that a number of factors affected accurate seizure detection including factors related to interpreter's experiences, display size and type of DTA methods used for analysis in addition to baseline EEG findings. ET was more dependent on user experience, furthermore, display size and multimodal DTA application (CSA and ET combined) increased the sensitivity of seizure detection for the experienced user compared to inexperience users. The artifacts were reported as seizures regardless of experience without presence of conventional EEG recording. The maximum spike amplitude, seizure duration, and seizure frequency were other important determinants for accuracy. Electrographic seizures with shorter duration were better detected by ET, and the maximum spike amplitude was important for both the ET and CSA. Repetitive seizures are readily detected by both digital trending methods. Artifacts may be reported as seizures regardless of experience if conventional EEG recording is not available for the interpretation. CONCLUSION: DTA applied to the raw EEG data does produce a graphic display that facilitates identification of seizures. The actual characteristics of the electrographic seizure may predict which DTA method is better and the overall accuracy of seizure detection may increase when multimodal trending is used simultaneously. Application of DTA alone with display of conventional EEG is beneficial for rapid interpretation of EEG findings regardless of experience.

Functionally oriented rehabilitation program for patients with fibromyalgia: preliminary results.

OBJECTIVE: To evaluate function and disability in patients with fibromyalgia before and after participation in a functionally oriented, multidisciplinary, 8-wk treatment program. DESIGN: A total of 23 patients who met American College of Rheumatology criteria for the diagnosis of fibromyalgia were enrolled in the study. Outcome measures included: range of motion, 6-min walk test, a modified Fibromyalgia Impact Questionnaire, a modified SF-36 Physical Functioning Scale, and the Fibromyalgia Health Assessment Questionnaire. Pretreatment and posttreatment scores were analyzed using paired t tests. RESULTS: All subjects completed the program, and there were no reported injuries. Three subjects failed to complete the survey instruments at the conclusion of the study. Intention to treat analysis including these subjects was carried out but did not significantly change results. For the remaining subjects (n = 20), a significant improvement was found on the Physical Functioning Scale (P = 0.01). Trends toward improvement on the Fibromyalgia Impact Questionnaire (P = 0.40) and Fibromyalgia Health Assessment Questionnaire (P = 0.14) were seen but did not achieve statistical significance. Range of motion testing revealed significant improvements in lumbar spine extension (P < 0.001), straight-leg raise (P < 0.001), cervical spine flexion (P < 0.01), cervical spine rotation (P < 0.05), and cervical spine side bending (P < 0.05). Distance traveled during the 6-min walk test increased significantly (P < 0.01), whereas perceived exertion as measured by the Borg scale did not change. There were no injuries or other adverse consequences of the program. CONCLUSIONS: This study utilized multiple functional outcome measures to demonstrate improved function and decreased disability in patients with fibromyalgia. Our patients reported significantly improved physical function after participation in the 8-wk intensive multidisciplinary treatment program. This progressive, functionally based exercise training program was well tolerated by all participants and outlines an effective exercise prescription for patients with fibromyalgia. Fibromyalgia patients in this study responded favorably to a treatment program that focused on function instead of pain.

Enzyme-linked immunosorbent assay for ultratrace determination of antibiotics in aqueous samples.

Two commercially available enzyme-linked immunosorbent assay (ELISA) kits that are commonly used for tylosin or tetracycline residues in meat and milk were adapted for ultratrace analysis of these antibiotics in surface and ground waters. These two antibiotics are commonly fed to swine, turkeys, and cattle at subtherapeutic doses for growth promotion purposes. Both ELISA techniques were found to be highly sensitive and selective for the respective antibiotics with detection limits of 0.10 and 0.05 microg L(-1) for tylosin and tetracycline, respectively. The recovery of both tylosin and tetracycline from spiked samples of lake waters, runoff samples, soil saturation extracts, and nanopure water was close to 100%. Tetracycline ELISA was highly specific for tetracycline and chlortetracycline but not for other forms of tetracycline (oxytetracycline, demeclocycline, and doxycycline). Analysis of a few liquid swine manure samples by liquid chromatography-mass spectrometry (LC-MS) showed lower concentrations for chlortetracycline as compared with concentrations obtained using ELISA. However, the concentrations of tylosin from ELISA were comparable with that of LC-MS. The lower concentrations of chlortetracycline obtained by LC-MS in manure samples indicate the presence of other similar or transformed compounds that were detected by ELISA but not determined by LC-MS. These results indicate that both ELISA kits can be useful tools for low-cost screening of tylosin, tetracycline, and chlortetracycline in environmental waters. Furthermore, both ELISA procedures are rapid, portable, and easily adaptable for testing of multiple samples simultaneously.