Blood glucose, amino acid profiles and nutrient transporter gene expressions in the small intestine of low and normal birthweight piglets during the early suckling period.

Birthweight and age can play a role in adaptational changes in plasma levels of glucose and amino acids by regulating the intestinal expression of specific nutrients transporters in piglets. The aim of the present study was to compare hematological features, circulating levels of glucose and amino acids, and small intestinal gene expression for transporters for glucose (SGLT1, GLUT2), amino acids (LAT2, b0,+AT) and peptides (PepT1) between low birthweight (LBW) and normal birthweight (NBW) piglets at birth prior to suckling and day 7 after birth. A secondary aim was to evaluate correlations between circulating levels of glucose or amino acids and their transporters. There were significant age-dependent changes in the immune cell repertoire in the circulating blood (P<0.004). Blood glucose levels were lower in LBW piglets than in NBW piglets (P<0.05); in both birthweight groups, blood glucose increased with age. Plasma concentrations of histidine, isoleucine, proline and tryptophan showed significant changes with age (P<0.003). With regard to mRNA levels for the nutrient transporters, there were no differences between the two birthweight groups or between the two age groups. At day 7 postpartum, intestinal expression of SGLT1 or GLUT2 mRNA did not correlate with blood glucose level. Expression of LAT2 mRNA in the ileum correlated positively with plasma level of methionine (r=0.92, P<0.001). This is the first detailed analysis of hematological features, plasma glucose and amino acid levels, and nutrient transporter expression in LBW and NBW piglets during the early suckling period.

Utilization of intestinal triglyceride-rich lipoproteins in mammary gland of cows.

Elution profiles of total lipoproteins, apolipoprotein B (apoB) concentrations in lipoproteins, and plasma triglyceride (TG) levels were examined in early-, late-, and non-lactating cows. Additionally, arteriovenous (A-V) differences were also measured to elucidate the uptake of TG and apoB-containing lipoproteins in mammary gland. Non-lactating cows showed three major peaks corresponding to triglyceride-rich lipoprotein (TRL), low density lipoprotein (LDL), and high density lipoprotein (HDL) fraction, whereas both early- and late-lactating cows revealed two peaks corresponding to TRL and HDL. The peak area of TRL in early- and late-lactating cows were significantly (p < 0.05) smaller than that in non-lactating cows. The plasma TG levels and apoB-48 concentrations of TRL in early- and late-lactating cows were also significantly (p < 0.01) lower. Furthermore, early lactating cows showed significantly (p < 0.05) larger A-V differences in both plasma TG and apoB-48 concentration of TRL than those in late- and non-lactating cows. These results suggested that TG in exogenous (intestinal) TRL was utilized for milk fat synthesis in lactating mammary gland of cows by the receptor-mediated uptake.

Proline, leucine, and alanine transport in placental microvillous membrane vesicles prepared from late gestational rats.

To characterize the active transport of amino acids across the placenta, uptakes of proline, leucine, and alanine were kinetically examined in placental microvillous membrane vesicles (PMV) prepared from rats in the late gestational period. Uptake rates of these amino acids in PMV showed saturable hyperbolic curves that obeyed Michaelis-Menten kinetics. Proline, leucine, and alanine transport were demonstrated to be carrier mediated systems with sodium-dependent, -independent, and both manner, respectively. In addition, sodium-dependent L-alanine transport showed two different systems, and new sodium-independent alanine transport system (K(m) of 1.12 mM) was observed in rat placenta. From these results, rat placenta has carrier mediated amino acid transport systems, and possesses at least three different transport systems for alanine.