ABSTRACT
OBJECTIVES: This study aimed to investigate if single nucleotide polymorphisms (SNPs) in a series of inflammatory genes were associated with the development of the most common pathologies thought to precede gastric cancer development namely; Helicobacter pylori (H. pylori)-associated gastritis and intestinal metaplasia. METHODS: A total of 250 patients were genotyped for 11 SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes. The study population comprised H. pylori uninfected ('normal') control patients (n=96), H. pylori-positive gastritis (n=91) and intestinal metaplasia patients (n=63). Genotyping was performed using Taqman allelic discrimination assays. Odds ratios for gastric disease groups were adjusted for potential confounding factors. RESULTS: No differences were identified in frequency of carriage, or homozygosity, for any of the 'risk' alleles investigated across the patient groups. No evidence was found to suggest an association with increased risk of developing either chronic gastritis or intestinal metaplasia with SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes or haplotypes tested. CONCLUSION: This study found no evidence of an association with increased risk of developing either chronic gastritis or intestinal metaplasia with the SNPs or haplotypes tested.
Subject(s)
Cytokines/genetics , Gastritis/genetics , Inflammation Mediators/physiology , Polymorphism, Single Nucleotide , Adolescent , Adult , Aged , Aged, 80 and over , Female , Gastric Mucosa/pathology , Gastritis/microbiology , Genetic Predisposition to Disease , Genotype , Helicobacter Infections/complications , Helicobacter pylori/isolation & purification , Humans , Interleukin 1 Receptor Antagonist Protein/genetics , Interleukin-10/genetics , Interleukin-1beta/genetics , Male , Metaplasia/genetics , Middle Aged , Precancerous Conditions/genetics , Stomach Neoplasms/genetics , Toll-Like Receptor 4/genetics , Tumor Necrosis Factor-alpha/genetics , Young AdultABSTRACT
BACKGROUND: Genes in the class III region of the MHC, encoding proteins involved in inflammation and vascular regulation, were investigated for association with the occurrence of vasodilation and requirement for vasopressor infusion. METHODS: A cohort of 236 elective cardiac surgical patients was studied. Hemodynamic and metabolic variables and dosage of vasopressor medications were recorded for the first 12 hours of intensive care unit admission after cardiac surgery on an electronic patient record. Demographic factors and operative details were recorded from other institutional databases. The DNA was extracted from peripheral blood mononuclear cells and genotyped for the presence of polymorphic alleles in genes coding for inflammation-related proteins. RESULTS: Carriage of the dimethylarginine dimethylaminohydrolase II (DDAH II) -449 G allele and the lymphotoxin alpha +252 G allele was significantly less frequent in patients who required infusions of vasopressors after cardiac surgery. On multivariate analysis, prior myocardial infarction, prolonged bypass, and the homozygous carriage of the DDAH II C allele were associated with postoperative vasopressor requirement. CONCLUSIONS: Vasopressor requirement after surgery may be related to an interaction of genotype, preoperative morbidity, and prolonged surgery.
