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J Pharm Sci
; 73(9): 1311-3, 1984 Sep.
Article
in English
| MEDLINE
| ID: mdl-6491961
ABSTRACT
A simple and rapid method for quantitating acetylcholine in a lyophilized preparation by high-performance liquid chromatography (HPLC) is described. A reverse-phase column with a refractive index detector was utilized for the assay. The HPLC system was able to separate acetylcholine from choline, a major degradation product, which was verified by running a degraded sample of a commercial preparation. The HPLC results were compared with the results obtained by a spectrophotometric procedure.