[Bion-M1. Biological activities of microorganisms under the conditions of a 30-day space flight].

It was stated that spaceflight factors (SFF) affect the chromosomal DNA interchange during Streptomyces crossing. Cross polarity and primary input of a parent chromosome fragment in recombinant generation imply a more lasting cells contact in microgravity and a broader horizontal transport of genetic material. SFF had no effect on recombination frequency and mutation in a model of parental auxotrophic markers reversion to prototrophism. It was demonstrated that SFF boosted the fC31 phage exit from S. lividans 66 (fC31) and did not influence phage induction in S. coelicolor A3(2) (fC31). SFF inhibited synthesis of antiobiotic actinorhodin in lisogenic S. coelicolor A3(2), and tylosin and desmicosin in S. fradiae. Survivability of electrogenic bacteria Shewanella oneidensis MR-1 in space flight was higher compared with the synchronous control experiment. The reduction activity of S. oneidensis MR-1 as an indicator of electron generation effectiveness was identical in flight and laboratory samples.

[Pigmented basidiomycete yeasts are a promising source of carotenoids and ubiquinone Q10].

Strains of basidiomycete yeasts isolated from different sources were studied in order to determine the content of carotenoid pigments and ubiquinone Q10 for subsequent selection work to obtain producers of these substances. High specific productivity of carotenoids (600-700 mg/g) was revealed in the representatives of the following species: Cystofilobasidium capitatum. Rhodosporidium diobovatum, R. sphaerocarpum. Rhodotorula glutinis, Rh. minuta, and Sporobolomyces roseus. The ratio of the major pigments (torulene, torularhodine, and beta-carotene) in the representatives of different species was studied. Certain specific features of pigment formation in relation to the taxonomic position of the yeasts were determined. Eurybiont species with substantial ecological lability are the most active producers of carotenoids and ubiquinone Q10 among the epiphytes. It is the first time a comparative analysis of the coenzyme Q10 content in different taxa has been performed using several strains of the same species. The maximal coenzyme Q10 production (1.84 mg/g of dry biomass) was found in the yeast species R. sphaerocarpum.

[Isolation of peptide antibiotic virginiamycin components and selection of their producer Streptomyces virginiae]. / Vydelenie komponentov peptidnogo antibiotika virdzhiamitsina i selektsiia ego produtsenta--Streptomyces virginiae.

A method for chromatographic separation and quantitative determination of individual components of the antibiotic virginiamycin, produced by microbiological synthesis (Streptomyces virginiae strain 147), is described. The components, M1-2 and S1-5, were isolated from fermentation broth and identified by HPTLC and HPLC (the results obtained using the two methods correlate well with each other). Conditions of culturing of the producer and compositions of nutritive media were optimized. Using UV irradiation as a mutagenic factor, the producer was selected for increased level of synthesis of the antibiotic; this was achieved by inducing mutations that impart resistance to virginiamycin and meta-fluorophenylalanine, an analog of phenylalanine.

[2 types of molecular structure (composition) of a genome in one species of transposable bacteriophages of Pseudomonas aeruginosa]. / Dva tipa molekuliarnoi struktury (sostava) genoma v predelakh odnogo vida bakteropfagov-transpozonov Pseudomonas aeruginosa.

Comparison of heteroduplexes (HD) between DNAs of different transposable phages of Pseudomonas aeruginosa belonging to two previously described subgroups (D3112 and B3) revealed two types of structure (composition) of the bacteriophages, designated "type A" and "type B". The properties of genome structure of type A (phages of D3112 subgroup) are as follows: high level of conservation (up to 70% of genomes of different phages are represented as blocks of homologous DNA sequences); substitutions in genomes revealed as nonhomology regions in HD are, as a rule, small and located in certain sites; the distribution of the nonhomologous regions in HD of these phages is highly reproducible in independent experiments. Bacteriophages of subgroup B3 have genomes of type B: only a small part (approx. 30%) of genomes retain homology general for all of the phages; the nonhomologous regions are distributed in a large number of sites in HD; the sizes of nonhomologous regions are substantially larger than for the phages of subgroup D3112; distribution of the regions in HD is highly variable, which is characteristic of DNAs with partial homology. There is no difference between genomes of types A and B in G + C content (approx. 61-63%). Viable recombinants can be formed in crosses between phages of different genome types not only in regions with earlier revealed large DNA/DNA homology (right ends of genomes), but also in central portions of the genomes. Nevertheless, functional incompatibility of some regions of phage genomes of types A and B was demonstrated.

[Interaction of tripeptides containing arginine and tyrosine (or phenylalanine) residues with synthetic polynucleotides and DNA of various origins]. / Issledovanie vzaimodeistviia tripeptidov, soderzhashchikh ostatki arginina i tirozina (ili fenilalanina), s sinteticheskimi polinukleotidami i DNK razlichnogo proiskhozhdeniia.

Model peptides--L-Arg-Gly-L-Arg, L-Arg-L-Tyr-L-Arg and L-Arg-L-Phe-L-Arg bind to different DNAs and synthetic polynucleotides and are found in the major groove of the double helix. Polynucleotide complexes containing L-Arg-Gly-L-Arg were studied in order to consider the influence of the arginine residues on the polynucleotide melting temperature. It was shown, that L-Arg-L-Tyr-L-Arg and L-Arg-L-Phe--L-Arg lowers the melting temperature in all polynucleotides studied. The dependence of the melting temperature of polynucleotide (DNA)--L-Arg-L-Tyr(L-Phe)-L-Arg complexes upon the polynucleotide GC-content has been detected. These effects reflect the intercalation of peptide tyrosyl (or phenylalanyl) residues into the double-stranded polynucleotide.

[Interaction of the peptide antibiotic bacitracin with DNA and synthetic polynucleotides]. / Issledovanie vzaimodeistviia peptidnogo antibiotika batsitratsina s DNK i sinteticheskimi polinukleotidami.

The aim of the present paper was to study the specific character of interaction of peptide antibiotic bacitracin with DNA and to estimate the interaction constant. The influence of bacitracin on bacteriophage DNA restriction by HindIII and SmaI endonucleases was studied. The possibility of arranging the polynucleotide template by small ligands was shown.

[Sporulation in Bacillus licheniformis during altered bacitracin synthesis]. / Sporoobrazovanie u Bacillus licheniformis pri izmenenii sinteza batsitratsina.

Sporulation in different strains of Bacillus licheniformis, 10716 and 1001 in connection with changes in synthesis of bacitracin was studied. It was shown that the sporulation efficiency did not depend on the synthesis of the antibiotic: in some strains with low potency for the antibiotic production, the sporulation level was lowered, while in the others, it was not lowered. Moreover, normal sporulation was also observed, when the synthesis of bacitracin was inhibited. Therefore, it is suggested that there is no correlation between the sporulation and antibiotic production.

[Study of the mechanism of the unwinding effect of destabilized helix of bacteriophage f1 gene 5 protein with the aid of model tripeptides]. / Issledovanie mekhanizma raspletaiushchego deistviia destabiliziruiushchego spiral' belka gena 5 bakteriofaga f1 s pomoshch'iu model'nykh tripeptidov.

Complexes of synthetic double-stranded polynucleotides and DNA with model peptides--L-Lys-L-Tyr-L-Lys and L-Lys-Gly-L-Lys have been investigated, using UV-spectroscopy. Polynucleotide complexes containing L-Lys-Gly-L-Lys were studied in order to consider the influence of lysyl residues on the polynucleotide melting temperature. It was shown, that L-Lys-L-Tyr-L-Lys lowers the melting temperature of all the polynucleotides studied, except poly(rI) . poly(C). The dependence of melting temperature of polynucleotide (DNA) . L-Lys-L-Tyr-L-Lys complexes upon the polynucleotide double helix form and GC-content has been detected. These effects have reflected the intercalation of peptide tyrosyl residues into one of the chains of the double-stranded polynucleotide. Correlation o the melting temperature dependences of polynucleotide complexes with gene 5 protein and L-Lys-L-Tyr-L-Lys upon polynucleotide double helix form and GC-content was found.

[Study of fusion of bacteriophage f2 double-stranded RNA, poly(A).poly(U), and poly(G).poly(C) in the presence of tetraethylammonium bromide]. / Issledovanie plavleniia dvukhtsepochechnoi RNK bakteriofaga f2, poli (A).polu(U) and poli(G).poli (C) v prisutstvii bromistogo tetraétilammoniia.

The data on the dependence of the melting curve parameters of double-stranded RNA (replicative form of RNA of f2 bacteriophage) poly(A) times poly(U) and poly(G) times poly(C) on the concentration of (C2H5)4NBr were obtained. The RNA melting range width is shown to pass through the minimum value T =2.1+/-0.1degrees at the point of inversion of relative stability of GC and AU pairs that corresponds to 4.0+/-0.1 M concentration of (C2H5)4NBr. Using the melting temperatures of poly(A) times poly(U) and poly(G) times poly(C) the rependence of Tgc-Tau parameter on (C2H5)4NBr concentration was shown. It was concluded from these data that the effect of the double-stranded RNA stacking heterogeneity was negligible in the 0-3 M range of (C2H5)4NBr concentration. Melting curves of RNA were obtained at various values of Tgc-Tau parameter. It was shown that the profile of fine structure of melting curves depends on the value of Tgc-Tau parameter.

[Certain physico-chemical properties of bacteriophage phiKZ]. / Nekotorye fiziko-khimicheskie svoistva bakteriofaga phiKZ.

Using UV-spectroscopy and circular dichroism (CD) phi KZ bacteriophage and its DNA were investigated. From the value of optical densities in the 320--400 nm range the size of the phi KZ bacteriophage's head was determined; the diameter of phi KZ bacteriophage head was found to be equal to 1300 +/- 100 A. phi KZ bacteriophage DNA has block structure and the GC-pair content is equal to 43.8 +/- 0.3%. phi KZ bacteriophage CD spectrum has an unusual profile (in comparison with known bacteriophage CD spectra); this spectrum is similar to the CD spectra of DNA in polyethylene glycole solution. To our knowledge CD spectra of such type were not obtained for other bacteriophages.

[Circular dichroism study of the reaction between the protein product of bacteriophage f1 gene 5 and synthetic polynucleotides]. / Izuchenie vzaimodeistviia belka--produkta gena 5 bakteriofaga f1 s sinteticheskimi polinukleotidami metodom krugovogo dikhroizma.

The complexes of gene 5 protein (phage f1) with single-stranded and double-stranded polynucleotides were investigated by circular dichroism (CD). In our experiments the concentration of the protein varied accordingly to polynucleotide. A decrease of the CD amplitude for polynucleotide-protein complexes was shown in all the regions of wavelength studied. The data indicate that the protein is bounded to the polynucleotide. This protein bounds differently to double-stranded polynucleotides containing ribo-ribo, ribo-deoxyribo and deoxyribo-deoxyribo chains. This difference is explained by differences in the form of the secondary structure of polynucleotides, containing ribo-ribo, ribo-deoxyribo and deoxyribo-deoxyribo chains.

[Unwinding effect of F1 gene 5 protein on double-stranded polynucleotides and DNA]. / K voprosy o mekhanizme raspletaiushchego deistviia belka gena 5 faga fl.

The effect of gene 5 protein from bacteriophage f1 on melting of double-stranded polynucleotides and DNAs has been investigated using the UV-spectroscopy method. A dependence of the melting temperature of polynucleotide (DNA)-gene 5 protein complexes upon the polynucleotide (DNA) GC-pair content has been detected. Using experimental data and examining some model systems we came to the supposition that the lowering of melting temperature of polynucleotide (DNA) induced by this protein is probably stipulated by intercalation of the protein tyrosyl residues into one of the chains of polynucleotide (DNA) double helix.

[Effect of the protein product of phage f1, gene 5, on heat denaturation of synthetic polynucleotides]. / Vliianie belka--produkta gena 5 faga f1--na teplovuiu denaturatsiiu sinteticheskikh polinukleotidov.

The influence of phage f1 gene 5 protein on melting of the synthetic polynucleotides has been investigated, using UV-spectroscopy. In our experiments we have varied the proteins concentration. It has been shown, that the protein lowers the melting temperature of the studied polynucleotides (d/A--Tn dAndTn, rAndTn, rAn.r n, dAn.rn). The melting temperatures and the shapes of melting curves of various polynucleotides differ when the same protein concentrations are used. We have shown that the protein binds to the double-stranded polynucleotides, containing ribo-ribo-, deoxyribo-ribo-chains. The difference in melting temperatures and shapes of melting curves was explained using the data about the differences in the secondary structure of these polynucleotides. Only for d/A-Tn renaturation was observed after sample cooling. It may reflect the single-stranded hairpin structure of this polynucleotide.

[Nuclear magnetic resonance of nucleotidamides. Structure of the intramolecular complex of the methyl ester of deoxyadenylyl-(5'-3')-deoxyadenylyl-(5'--N)-L-phenylalanine]. / Iadernyi magnitnyi rezonans nukleotidamidov. Struktura vnutrimolekuliarnogo kompleksa metilovogo éfira dezoksiadenilil-(5'-3')-dezoksiadenilil-(5'--N)-L-fenilalanina.

The secondary structure of methyl ester of deoxyadenylyl-(5'--3')-deoxyadenylyl-(5' leads to N)-L-phenylalanine [l-pheOMe-d (pApA)] has been investigated by proton magnetic resonance. For this compound folded conformation stabilized by hydrophobic interaction in aqueous solution has been shown; and the anti-anti-conformation has been proved to exist. The investigation of chemical shifts of aromatic protons in L-PheOMe-d (pApA) has permitted us to build the conformation model of this compound.

[The secondary structure of amides of adenylic acid containing D- and L-aromatic amino acids]. / Osebennosti vtorichnoi struktury amidov adenilovoi kisloty, soderzhashchikh D- i L-aromaticheskie aminokislety

Adenylyl-(5'leads to N)-amino acids containing as amino components, methyl esters of D-, L- and DL-phenylalanine, D-, L- and DL-tyrosine, and D-, L- and DL-tryptophan have been investigated by proton magnetic resonance (PMR) spectroscopy and circular dichroism (CD). The temperature and pD dependences of proton chemical shifts of these compounds have been studied. These data, together with the magnitudes of the upfield chemical shifts of the PMR signals of adenine and aromatic amino acids residues in adenylyl-(5'leads to N)-amino acids, have enabled us to construct conformational models of these compounds. The proposed conformation has been substantiated by the CD results. It is shown that in adenylyl-(5'leads to N)-amino acids the planes of adenine and amino acid aromatic moieties are roughly parallel. The aromatic rings of phenylalanine and tyrosine are localized approximately above the centre of adenine. In adenylyl-(5'leads to N)-D, -(L)-tryptophan, the six-membered rings of the indole overlaps the five-membraned ring of adenine indole partially overlaps the six-membered ring of adenine. A difference in the non-covalent interactions of D- and L-amino acids with nucleotides has been revealed. The mutual localization of the aromatic systems of AMP and the amino acids and also the positions of -OCH3 group with respect to the centre of the amino acid aromatic moiety differs in the series of the studied nucleotide derivatives of D- and L-amino acids.

[The secondary structure of nucleotidyl-(5' bound to N)-amino acids containing different heterocyclic bases and amino acids]. / Issledovanie vtorichnoi struktury nukleotidil-(5' leads to N)-aminokislot s razlichnoi prirodoi osnovaniia i aminokisloty

Nucleotidyl-(5' leads to N)-amino acids containing different heterocycle bases: adenine, guanine, hypoxanthine, cytosine, uracyl, and aromatic amino acids: phenylalanine, tyrosine and tryptophan, have been investigated by proton magnetic resonance and circular dichroism. For all the compounds studied folded conformation have been shown stabilized by hydrophobic interaction in aqueous solution. The comparison of the results of the studied nucleotidyl-(5' leads to N)-amino acids unable us to build four secondary structure types in these very compounds. Phenylalanine and tyrosine derivatives of purine nucleotides can be regarded as the first type, tryptophan derivatives of purine nucleotides as the second type, phenylalanine and tyrosine derivatives of pyrimidine nucleotides as the third type and tryptophan derivatives of pyrimidine nucleotides as the fourth type. For each group of these compounds conformational models have been built. In all these compounds the anti-conformation has been proved to exist.