[Structure-function relationship between analogues of the antibacterial peptide indolicidin. I. Synthesis and biological activity of analogues with increased amphipathicity and elevated net positive charge of the molecule]. / Vzaimosviaz' struktura-aktivnost' v riadu analogov antibakterial'nogo peptida indolitsidina. I. Sintez i biologicheskaia aktivnost' analogov s uvelichennoi amfipatichnost'iu i povyshennym obshchim polozhitel'nym zariadom molekuly.

The antibacterial peptide indolicidin and a number of its analogues were obtained by solid phase synthesis. An optimized method of the synthesis using the Boc strategy was suggested. It was shown that the therapeutic index of indolicidin analogues increased with a decrease in the total positive charge of the molecule and its amphipathicity; i.e., the hemolytic activity of analogues within the range of concentrations examined was practically absent, while the antibacterial activity was preserved. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.

[Non-specific changes in the complement system in neoplastic processes]. / Nespetsificheskie izmeneniia sistemy komplementa pri neoplasticheskikh protsessakh.

The study using immunochemical and biological methods was concerned with assaying complement components C1q, C3, C4, and C5, C3 derivatives--C3a and, C3(H2O), C1ing control protein, hemolytic activity (CH50), total proteolytic activity and regulatory immune complexes in sera from patients with cancer of the stomach, breast and ovary, subjects at risk of cancer and healthy donors. Neoplasia was associated with lowered levels of intact C3 and C1ing matched by high concentrations of C3a, C1q and C4. Hemolytic activity (CH50) was increased against the background of relatively higher total proteolytic activity both in healthy subjects and cancer patients. Significant decrease in C1ing concentration may be used as an additional biochemical prognosticator in breast cancer.

[Conformational changes in C3 levels of complement during storage of blood from Hodgkin's disease patients and from healthy people]. / Konformatsionnye izmeneniia C3-komponenta komplementa pri khranenii krovi bol'nykh limfogranulematozom i zdorovykh liudei.

Storage-related differences in C3 level of blood complement and its hydrolized form--C3(H2O)--were identified in Hodgkin's disease patients and healthy donors, by immunoenzymatic analysis using murine monoclonal antibodies. Both C3 and C3(H2O) levels in blood serum of patients varied with time and were significantly different from those in health subjects; they correlated with EDTA concentration. After a second thawing of plasma in patients, there were no traces left of C3(H2O).

[Experimental study of the biopreparation Lymphotilin as an antiproliferative and antitumor agent]. / Eksperimental'noe izucheniie biopreparata Limfotilin kak antiproliferativnogo i protivoopukholevogo sredstva.

We found in experiments involving the use of a biopreparation lymphotilin that its administration was followed by a decrease in proliferative activity of cultured tumor cells and a longer survival of mice bearing transplantable leukemia. An intensified intercalation of ethidium bromide in nucleic acids of tumor cells in lymphotilin culture points to the drug activity on nuclear level. Tumor cell inhibition by lymphotilin holds much promise for the practice of hematology.

[Modification of complement components under the effect of cationic proteins from leukocytes from donors and leukemia patients]. / Modifikatsiia komponentov komplementa pod deistviem kationnykh belkov leikotsitov donorov i bol'nykh leikozami.

Cation proteins (CP) were isolated from leukocytes by means of ion exchange chromatography. Enzyme immunoassay showed that these cation proteins modified C3 component of complement in vitro. CP obtained from healthy volunteers decreased the content of C3 purified by affinity chromatography and C3 of blood serum; however, content of C3.H2O op C3b-like form and C3a-fragment were not altered. Effect of CP isolated from leukocytes of patients with chronic myeloleukemia and chronic lympholeukemia on C3 component was less distinct as compared with that of healthy volunteers. At the same time, content of C3b-like form was increased the presence of CP from patients with myeloleukemia and of C3a-fragment--in the presence of the proteins from patients with lympholeukemia. Excess of physiologically active C3 fragments, developed under conditions of these diseases, may contribute to a decrease in unspecific body resistance.

[Effect of lysosomal acid-soluble proteins on the respiratory activity of the granulocytes of healthy subjects and chronic myeloid leukemia patients]. / Vliianie lizosomal'nykh kislotorastvorimykh belkov na dykhatel'nuiu aktivnost' granulotsitov zdorovykh liudei i bol'nykh khronicheskim mieloleikozom.

A rate of oxygen consumption by granulocytes was measured before cell stimulation and following their stimulation by zymosan in healthy persons and in patients with chronic myelocytic leukemia (CML). The rate of oxygen consumption by granulocytes of donors was higher than that of patients with CML. Addition of lysosomal acid soluble proteins, extracted from normal granulocytes, affected the respiratory activity of stimulated and non-stimulated both normal and leukemic cells, depending upon the protein concentration. The alterations were more marked in granulocytes of donors. Lysosomal acid soluble proteins, extracted from granulocytes of patients with CML did not change the cell respiratory activity.

[Change in specific antigen binding by rabbit lymphocytes during immunization with human serum albumin]. / Izmenenie spetsificheskogo sviazyvaniia antigena limfotsitami krolika v protsesse immunizatsii syvorotochnymi al'buminom cheloveka

Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.