[The experience of abundant amount of pectoralis major myocutaneous flap tissue in restoring parenchyma defect of head and neck].

Objective:Explore the skills to flap the defects of the head and neck with myocutaneous of pectoralis major which has rich amount of organization to minimize complications.Method:Various of neck defects will be repaired when the myocutaneous flap of pectoralis major from obese male or/and female has been prepared with improved methods. First of all, the muscle must be fixed. Then we have to suture the skin and the defect of skin or mucous membrane;the potential closed cavities around the myocutaneous flap must be drainaged with the negative pressure after repaired. Clinical data of 23 (24 sides) patients undergoing the repair in our hospital between January 2008 and December 2014 were collected and reviewed retrospectively.Result:One of 23 cases was injured the thoracoacromial artery in the preparation process of myocutaneous flap by the operator, then we operated on the other side. All of the pectoralis major myocutaneous flaps has been survived except 1 case which was necrosis in distal partial of pectoralis major muscle flap, but healed after changing dressing.Three cases with pharyngeal leakage because of the infection were also healed after changing dressing; skin and skin flap partial dehiscence happend in 1 cases, but also healed after dressing; 1 cases with subcutaneous hemorrhage was healed after cleaning up hematocele.Conclusion:The pectoralis major myocutaneous flap is still a good material for the repair of head and neck defects as long as the management skill in perioperative has been mastered properly, even though the one with rich amount of organization is bloated and the preparation and repair is difficult.

Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein.

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.

Polymorphism of ADH and ALDH genes among four ethnic groups in China and effects upon the risk for alcoholism.

The alcohol dehydrogenases (ADHs) and aldehyde dehydrogenases (ALDHs) that metabolize ethanol are polymorphic. Different alleles encode subunits of the enzymes that differ in their rate of metabolizing ethanol. These polymorphisms are distributed differently among populations and have been shown to influence the risk for alcoholism in some Asian populations. We have examined the allele frequencies at the ADH2, ADH3, and ALDH2 loci in four populations from China (Han, Mongolian, Korean, and Elunchun) and in alcoholics within each population. The four populations differ in allele frequencies, with the Elunchun having a much lower frequency of ADH2*2 alleles, and the Mongolian and Elunchun having a much lower frequency of ALDH2*2 alleles. Within each population, alleles at one or more of these three loci are protective against alcoholism, although the populations differ in which loci play significant roles. The protective allele at each locus (ALDH2*2, ADH2*2, and ADH3*1) encodes a subunit that either metabolizes ethanol to acetaldehyde more rapidly or slows the conversion of acetaldehyde to acetate. Taken as a whole, data demonstrate that genetic differences in the enzymes that metabolize alcohol can substantially affect the risk for alcoholism.