Moringa oleifera leaf polysaccharide regulates fecal microbiota and colonic transcriptome in calves.

This study investigated the effects of Moringa oleifera polysaccharide on growth performance indicators, serum biochemical indicators, immune organ indicators, colonic morphology, colonic microbiomics and colonic transcriptomics in newborn calves. 21 newborn calves were randomly divided into three groups of 7 calves per treatment group: control group (no Moringa oleifera polysaccharide addition); low-dose group (Moringa oleifera polysaccharide 0.5 g/kg); and high-dose group (Moringa oleifera polysaccharide 1 g/kg). This trial used gavage to feed MOP to calves. The test lasted 8 weeks. Calves were humanely electroshocked on the last day of the trial and slaughtered afterwards. Thymus, spleen, blood and colonic contents were collected for further testing. The results of this trial showed that MOP significantly increased the body weight of newborn calves and reduced the rate of calf diarrhea, thus promoting calf growth. Fecal scores showed a linear decrease with the addition of MOP. In terms of serum biochemistry, feeding MOP significantly increased serum ALB levels in a linear fashion. In terms of serum antioxidants, feeding MOP linearly increased CAT and T-AOC levels and decreased MDA concentrations, and in terms of serum immunity, feeding MOP linearly increased IgA, IgG, and IgM levels. At the same time, MOP regulated the abundance of Firmicutes and Bacteroidetes in the intestinal tract of calves, which reduced the occurrence of diarrhea. In addition, moringa polysaccharide could regulate genes related to inflammatory signaling pathways such as MAPK signaling pathway, TGF-beta signaling pathway, PI3K-Akt signaling pathway and TNF signaling pathway in calves' intestine to reduce the occurrence of intestinal inflammation. In conclusion, MOP can be used as a novel ruminant additive for the prevention of enteritis in calves.

A preliminary study on the possibility of fermented pineapple peel residue partially replacing whole corn silage in feeding Chuanzhong black goats.

This study aims to assess the effects of the partial replacement of whole corn silage (WCS) with fermented pineapple peel residue (FPPR) on growth, serological parameters, muscle quality, rumen microorganisms, and fecal microorganisms. A total of 24 Chuanzhong black goats weighing 10.23 ± 1.42 kg were evaluated in a randomized complete trial design in accordance with the following treatments: (1) 0% FPPR in the diet, (2) 25% FPPR in the diet, and (3) 50% FPPR in the diet. In goats, the partial substitution of FPPR for WCS increased the abundance of probiotics, such as Blautia, Butyrivibrio fibrisolvens, and Ruminococcus albus, and did not exert significant effects on overall serological parameters and muscle quality. In conclusion, the partial substitution of FPPR for WCS in the diet did not impair or affect the productive performance of goats.

Comprehensive analysis of microbiome, metabolome and transcriptome revealed the mechanisms of Moringa oleifera polysaccharide on preventing ulcerative colitis.

This study aimed to investigate the protective effect of Moringa oleifera polysaccharide (MOP) on ulcerative colitis (UC) and explore its mechanism through the combined analysis of microbiome, metabolome and transcriptome. A UC model in mice was established using dextran sulphate sodium. After a 21-day experiment, results showed that MOP could inhibit the weight loss and disease activity index in UC mice. The intervention of MOP decreased the expression of inflammatory cytokines and promoted the secretion of tight junctions. MOP could promote the growth of probiotics such as Lachnospiraceae_NK4A136, Intestinimonas and Bifidobacterium in UC mice. The results of metabolomic and transcriptomic analysis indicated that MOP could regulated the metabolism of polyunsaturated fatty acid and PPAR, TLR and TNF signalling pathways might play important roles in the process. Altogether, MOP could be used as a functional food to prevent UC.

Effects of Cellulase and Lactiplantibacillus plantarum on the Fermentation Parameters, Nutrients, and Bacterial Community in Cassia alata Silage.

Silage Cassia alata (CA) can alleviate feed shortage in some areas to a certain extent and reduce feed costs. The present research evaluated the effect of cellulase (CE) and Lactiplantibacillus plantarum (LP) on the fermentation parameters, nutrients, and bacterial community of CA silage. Chopped CA was ensiled with three different treatments, namely, no inoculant (CK), CE, and LP, and the indexes were determined on the 2nd, 6th, 14th, and 30th days of silage fermentation. The fermentation parameters indicate that the pH value of the three groups decreased and then increased with the ensilage process, and the lowest value was observed on the 14th day. The CK and LP groups attained the highest value on the 30th day, while the CE group attained the highest value on the 2nd day. Additionally, the pH value and NH3-N content were significantly lower (P < 0.05) in the CE and LP groups than in the CK group. In terms of nutrients, crude protein (CP) contents significantly increased (P < 0.05) in the CE and LP groups on the 30th day. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of the CE group were significantly and negatively associated with fermentation time, and the water-soluble carbohydrate (WSC) contents of the three groups were significantly lower during ensiling. In comparison with the CK group, the NDF and ADF contents were significantly reduced (P < 0.05), and the WSC content increased (P < 0.05) in the CE group on day 30. Sequencing analysis of bacterial communities showed that Lactobacillus became the most dominant genus in the ensilage process. Moreover, both CE and LP groups increased the abundance of Lactobacillus and decreased that of Klebsiella, Weissella, and Acetobacter in comparison to the CK group, in which LP had a better effect. CE and LP could further improve the silage quality of CA, and LP had a more significant effect in reconstructing the bacterial community in the silage environment.

Effects of Different Concentrations of Lactobacillus plantarum and Bacillus licheniformis on Silage Quality, In Vitro Fermentation and Microbial Community of Hybrid Pennisetum.

The purpose of the experiment was to study the effects of different concentrations of Lactobacillus plantarum (LP) and Bacillus licheniformis (BL) on the quality of hybrid Pennisetum (HP) silage. The experiment consisted of five treatment groups. The control group did not use additives, and the experimental groups were added with LP or BL of 1 × 105 cfu/g fresh weight (FW) and 1 × 107 cfu/g FW, respectively. The results showed that LP and BL could increase the in vitro fermentation gas production and reduce the ammonia nitrogen (AN) content in HP silage. Water-soluble carbohydrates (WSC), lactic acid (LA) content, and gas production in the LP group were positively correlated with LP addition, and acetic acid (AA) was negatively correlated with addition. The content of WSC and LA in the LP7 group was significantly higher than that in the control group (p < 0.05), and AA was lower than that in the control group (p > 0.05). Dry matter (DM), crude protein (CP), and gas production were negatively correlated with the addition of BL, while acid detergent fiber (ADF) content was positively correlated with the addition of BL. Furthermore, in the above indicators, the BL5 group reached a significant level with the control group (p < 0.05). The results of 16sRNA showed that the use of LP and BL could increase the relative abundance of Lactobacillus and decrease the relative abundance of Weissella in HP silage compared with the control group. In conclusion, LP and BL can significantly improve the quality of HP silage. The LP7 group and the BL5 group have the best silage effect. From the perspective of gas production in in vitro fermentation, the LP7 group had stronger fermentability and higher nutritional value.

Effects of Broussonetia papyrifera silage on rumen fermentation parameters and microbes of Holstein heifers.

The structure and types of rumen microbes are closely related to host health. This study aimed to evaluate the effect of Broussonetia papyrifera silage (BPS) gradually replacing the whole crop maize silage (WCMS) on total tract digestibility, rumen fermentation parameters, serum biochemical indicators, and rumen microbes of Holstein heifers. The diet treatment consisted of four proportions of BPS (0%, 25%, 50% and 75%) as substitute for WCMS (designated as T0, T25, T50 and T75, respectively). Twenty heifers (body weight = 245 ± 24 kg) were randomly divided into four groups of five heifers, and randomly received one diet. The feeding adaption period was 7 days, and the experiment period was 30 days. Our findings suggested that the digestibility of neutral detergent fiber and crude protein increased linearly with the increased in BPS (P < 0.05). The concentrations of total protein and albumin increased quadratically with the increased in BPS (P < 0.05). The 16s high-throughput sequencing showed that feeding BPS did not change the diversity and structure of the rumen microbes of heifers. However, the relative abundances of Tenericutes and SR1-Absconditabacteria increased linearly with the increased in BPS (P < 0.05). The Weighted Correlation Network Analysis results suggested that ALT concentration was positively correlated with the abundance of Prevotella-1 (r = 0.73; P = 0.007). In general, Holstein heifers fed with BPS did not change the diversity and uniformity of rumen microbes, and enhanced the body protein metabolism.

High-Entropy Perovskite as a High-Performing Chromium-Tolerant Cathode for Solid Oxide Fuel Cells.

To achieve chromium tolerance and high performance, a new series of high-entropy perovskites (HEPs) are investigated as cathode materials for solid oxide fuel cells (SOFCs). Multiple rare-earth, alkaline-earth, and high-order transition metal elements are used for the A-site of this ABO3 structure. A pure phase is achieved through the designed combination of different elements in seven out of eight candidates. Due to the retaining of alkaline-earth elements Sr and/or Ba, the electrical conductivities of these HEPs are in the order of 100 S/cm at 550-700 °C, a value that can practically eliminate the electronic resistance of the porous cathode. Three out of eight candidates show similar or better performance than the (La0.6Sr0.4)(Co0.2Fe0.8)O3-δ (LSCF) benchmark. It is found that A-site elements can cast a substantial influence on the overall performance even with a change as small as 10% of the total cations. It seems that each element has its individual "phenomenal activity" that can be transferred from one candidate to the other in the general setting of the perovskite structure, leading to the best candidate by using the three most active elements simultaneously at the A-site. Excellent Cr tolerance has been observed on the (La0.2Sr0.2Pr0.2Y0.2Ba0.2)Co0.2Fe0.8O3-δ sample, showing degradation of only 0.25%/kh during a 41 day operation in the presence of Cr, while LSCF increases by 100% within the first day in the same condition. X-ray photoelectron spectroscopy discovers no Sr segregation as LSCF is found in this HEP; rather, the active element Y takes more A-sites on the outermost layer after long-term operation.

Moringa oleifera polysaccharide regulates colonic microbiota and immune repertoire in C57BL/6 mice.

This study investigated the effects of Moringa oleifera polysaccharide (MOP) on serum immune indices, immune organ indices, colonic microflora and immune repertoire of mice. Forty male SPF C57BL/6 mice were randomly divided into four groups and subjected to gavage of 0, 20, 40 and 60 mg/kg MOP for 28 days. Mice were sacrificed on the last day of the experiment and their thymus, spleen, blood and colon contents were collected for further detection. Our findings suggested that MOP could significantly increase the thymus index (P < 0.01) and spleen index (P < 0.05), and significantly decrease the levels of interleukin-6 and tumour necrosis factor-α in mice (P < 0.05). And MOP could regulate the proportion of colonic microflora of mice, significantly increase the abundance of Muribaculaceae and significantly decrease the abundance values of Proteobacteria, Helicobacter, Stenotrophomonas, etc (P < 0.05). In addition, MOP could regulate the usage frequencies of TRBV15 (P = 0.06) and TRBV9 (P = 0.10) on the TCRα chain and 9 V-J pairs were found to have remarkable usage frequency changes. These results implied that MOP exerted positive effects on the immune performance and intestinal health of mice.

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage.

The present study investigated the effects of malic acid, sucrose, and their mixture on the fermentation parameters, Cornell Net Carbohydrate and Protein System (CNCPS) nitrogen fractions, and bacterial community of Moringa oleifera leaves (MOL) silages. The trial was divided into four treatments and labeled as CON (control group) and MLA, SUC, and MIX (respectively denoting the addition of 1% malic acid, 1% sucrose, and 1% malic acid + 1% sucrose to the fresh weight basis). The silage packages were opened on the 2nd, 5th, 10th, 20th, and 40th days of ensiling for subsequent determination. Malic acid and sucrose increased the lactic acid content (p < 0.05) and pH value, and the acetic acid contents of MLA and MIX were lower than those in CON (p < 0.05). Compared with sucrose, malic acid had a better capacity to preserve nutrients and inhibit proteolysis, and thus exerted better effects on the CNCPS nitrogen fractions. The results of 16S rRNA showed that the dominant phyla were Firmicutes and Proteobacteria and that the dominant genera were Lactobacillus and Weissella. With the application of silage additives and the processing of fermentation, there was a remarkable change in the composition and function of the bacterial community. The variation of the fermentation parameters and CNCPS nitrogen fractions in the MOL silages caused by malic acid and sucrose might be attributed to the dynamic and dramatic changes of the bacterial community.

Moringa oleifera polysaccharides regulates caecal microbiota and small intestinal metabolic profile in C57BL/6 mice.

This study investigated the effects of Moringa oleifera polysaccharides (MOP) on the serum indexes, small intestinal morphology, small intestinal metabolic profile, and caecal microbiota of mice. A new type of polysaccharides with 104,031 Da molecular weight and triple helix structure was isolated from M. oleifera leaves for in vivo experiment. Forty male SPF C57BL/6 mice aged 4 weeks were average divided into four groups randomly according to the MOP gavaged daily (0, 20, 40 and 60 mg/kg body weight MOP). After a 7-day preliminary trial period and a 28-day official trial period, the mice were slaughtered. Results showed that MOP reduced glucose, total cholesterol, and malondialdehyde. It also improved superoxide dismutase and catalase in serum (P < 0.05). For small intestinal morphology, MOP improved the villi length and crypt depth in both ileum and jejunum (P < 0.05); the ratio of villi length to crypt depth in jejunum increased (P < 0.05). MOP could cause the increase of beneficial bacteria and the decrease of harmful bacteria in caecum, further affecting the function of microbiota. In addition, MOP regulated 114 metabolites enriched in the pathway related to the synthesis and metabolism of micromolecules. In sum, MOP exerted positive effects on the serum indexes and intestinal health of mice.

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum.

This study was conducted to investigate the effects of different additives on the fermentation quality, nutrient composition, bacterial communities, and metabolic profiles of the silage of hybrid Pennisetum. The experiment was conducted using five treatments, i.e., CK, control group, MA, 1% malic acid of fresh matter (FM) basis, GL, 1% glucose of FM basis, CE, 100 U/g FM cellulase, and BS, 106 cfu/g FM Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 silage packages were opened for subsequent determination. As a result, all four additives had positive effects on the fermentation quality and nutrient composition of the silage of hybrid Pennisetum. The high-throughput sequencing of V3-V4 regions in 16S rRNA was performed, and results showed that Firmicutes and Proteobacteria were the dominant phyla and that Aquabacterium and Bacillus were the dominant genera. MA, GL, CE, and BS treatment resulted in 129, 21, 25, and 40 differential bacteria, respectively. The four additives upregulated Bacillus smithii but downregulated Lactobacillus rossiae. Metabolic profiles were determined by UHPLC-Q/TOF-MS technology and the differential metabolites caused by the four additives were 47, 13, 47, and 18, respectively. These metabolites played antioxidant, antibacterial, and anti-inflammatory functions and involved in pathways, such as the citrate cycle, carbon fixation in photosynthetic organisms, and glyoxylate and dicarboxylate metabolism. In conclusion, silage additives promoted fermentation quality and nutrient composition by altering bacterial communities and metabolic profiles. This study provided potential biomarkers for the improvement of silage quality.

Deconvolution of Water-Splitting on the Triple-Conducting Ruddlesden-Popper-Phase Anode for Protonic Ceramic Electrolysis Cells.

Triple-conducting materials have been proved to improve the performance of popular protonic ceramic electrolysis cells. However, partially because of the complexity of the water-splitting reaction involving three charge carriers, that is, oxygen (O2-), proton (H+), and electron (e-), the triple-conducting reaction mechanism was not clear, and the reaction conducting pathways have seldom been addressed. In this study, the triple-conducting Ruddlesden-Popper phase Pr1.75Ba0.25NiO4+δ as an anode on the BaCe0.7Zr0.1Y0.1Yb0.1O3-δ electrolyte was fabricated and its electroresponses were characterized by electrochemical impedance spectroscopy with various atmospheres and temperatures. The impedance spectra are deconvoluted by means of the distribution of the relaxation time method. The surface exchange rate and chemical diffusivity of H+ and O2- are characterized by electrical conductivity relaxation. The physical locations of electrochemical processes are also identified by atomic layer deposition with a surface inhibitor. A microkinetics model is proposed toward conductivities, triple-conducting pathways, reactant dependency, surface exchange and bulk diffusion capabilities, and other relevant properties. Finally, the rate-limiting steps and suggestions for further improvement of electrode performance are presented.

Effect of Broussonetia papyrifera silage on the serum indicators, hindgut parameters and fecal bacterial community of Holstein heifers.

This study investigated the effects of substitution of whole corn silage (WCS) with Broussonetia papyrifera silage (BPS) in different ratios on the serum indicators, hindgut fermentation parameters (pH, ammoniacal nitrogen, and volatile fatty acids), and fecal bacterial community of Holstein heifers. Sixteen heifers (8-month-old, 220 ± 30 kg) were randomly divided into four treatments according to different BPS substitution ratios of feed basis (0%, 25%, 50%, and 75%). The experiment consisted of a 7-day preliminary feeding period and a 30-day experimental period. On the last day of the trial, the blood samples were collected from caudal vein, and the feces samples were collected from rectum. With the increasing of BPS content, the concentration of malondialdehyde (MDA) and interleukin-1ß (IL-1ß) in serum decreased (P < 0.05), and the immunoglobulin A (IgA) and IL-4 content of serum increased (P < 0.05); and the hindgut pH value increased (P < 0.05). 16S rRNA sequencing found that the dominant phyla were Firmicutes, Bacteroidetes, and Verrucomicrobia; and the dominant genera were Ruminococcaceae_UCG-005, Ruminococcaceae_UCG-010, and Rikenellaceae_RC9_gut_group. Linear Discriminant Analysis Effect Size (LEfSe) analysis found 12 differential operational taxonomic units (OTUs) which have strong correlation with some serum and hindgut indicators, and have the potential to be used as biomarkers. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) found that BPS have impacts on the pathways, such as carbohydrate transport and metabolism, and promotes amino acid transport and metabolism. To sum up, inclusion of BPS in heifer diets can affect serum anti-oxidant and immune indicators, fecal parameters, composition and function of fecal microorganisms in Holstein heifers.

Lycopene supplementation regulates the gene expression profile and fat metabolism of breeding hens.

This study investigated the effects of lycopene on the gene expression profile and expression of genes related to fat metabolism of Xinghua breeding hens. Seven hundred and twenty healthy breeding hens were randomly assigned to four treatments; each treatment was replicated six times with 30 hens each. Broken rice and soybean meal were adopted for the basal diet and added with 0 (control group), 20, 40 and 80 mg/kg lycopene respectively. Gene expression profile of the liver induced by lycopene and expression of genes related to fat metabolism in hens liver and intestine were analysed after 42-day feeding trial including 7-day pre-feeding period and 35-day formal period. The genes involved in fat metabolism were analysed, and we found that lycopene significantly increased the expression of PGC1α, PPARα, RXRα and RARα in the liver, PPARγ, RXRα and RXRγ in the jejunum, and RARα in the duodenum (p < .05); reduced the expression of FABP1 and FABP10 in the liver, and FATP4 in the jejunum (p < .05). By analysing gene expression profile, 158 differentially expressed genes (DEGs) including 69 up-regulated genes and 89 down-regulated genes were obtained between control group and 40 mg/kg group. KEGG pathway analysis was performed on all DEGs, and 5 pathways were obtained. In conclusion, lycopene can affect the expression of related genes, and this may be one of the reasons that lycopene can regulate fat metabolism.

Synergistic Coupling of Proton Conductors BaZr0.1Ce0.7Y0.1Yb0.1O3-δ and La2Ce2O7 to Create Chemical Stable, Interface Active Electrolyte for Steam Electrolysis Cells.

For the first time, proton conductors BaZr0.1Ce0.7Y0.1Yb0.1O3-δ (BZCYYb) and La2Ce2O7 (LCO) are combined to create an interface active and steam-tolerant electrolyte for high-performance proton-conducting solid oxide electrolysis cells. LCO shows good chemical compatibility with BZCYYb. The readily fabricated LCO/BZCYYb bilayer electrolyte can be densified at a temperature of as low as 1300 °C versus ∼1600 °C for the benchmark steam-stable BaZr0.8Y0.2O3-δ electrolyte. With Pr2NiO4+δ as the anode and Ni as the cathode catalyst, this bilayer electrolyte cell yields a current density of 975 and 300 mA/cm2 under a 1.3 V applied potential at 700 and 600 °C, respectively. This performance is among the best of all H-SOECs equipped with a chemically stable electrolyte so far. A BZCYYb layer in the bilayer electrolyte promotes the hydrogen evolution reaction at the cathode side, resulting in a 108% improvement over the cell without this layer. The LCO layer, on the other hand, effectively protects this functional BZCYYb layer from the high concentration of steam in a practical SOEC operation condition. The cell without the LCO layer shows degradation in terms of an increased electrolyzing potential from 1.07 to 1.29 V during a constant 400 mA/cm2 operation at 700 °C. In contrast, the bilayer electrolyte cell maintains the same electrolyzing potential of 1.13 V under the same conduction for a 102 h operation. These findings demonstrate that this synergic bilayer electrolyte design is a vital strategy to overcome the dilemma between performance and stability faced by the current benchmark Zr- or Ce-rich Ba(CeZr)O3-δ electrolysis cells to achieve excellent performance and stability at the same time.