ABSTRACT
Candida albicans (C. albicans) is a common cause of vulvovaginal candidiasis (VVC). In this paper, the genetic diversity and molecular epidemiology of 173C. albicans strains were investigated by multilocus sequence typing (MLST). A total of 52 diploid sequence types (DSTs) were recognized, and 27 (51.9%) of which have not been reported in the MLST database. Genotyping was performed on the multiple isolates collected from patients with recurrent VVC (RVVC, referring to VVC which attacks more than 4 times in one year) in different acute infectious phases. The results showed that 59.1% (26/44) of the patients suffered a relapse, with DST 79 (65.4%) as the dominant genotype. The etiology of the remaining 40.9% (18/44) of patients was reinfection, and the main genotypes included DST 79 (33.3%), DST 124 (8.6%) and DST 1895 (8.6%). DST 79 (45%) and DST 1395 (7.5%) were the main isolates of VVC patients, while DST 79 (24.1%), DST 727 (6.9%), DST 732 (6.9%) and DST 1867 (6.9%) were the main types of healthy volunteers. The results of the genotypes between RVVC patients and other groups were statistically different. Furthermore, cluster analysis was carried out on 1468 isolates, among which 1337 were downloaded from the MLST database, 130 were divided into 8 Clades in the present study and the remaining one was taken as a singleton. 92.3% isolates from relapse patients, 58.3% isolates from re-infected patients, 77.5% isolates from VVC patients and 51.7% isolates from volunteers were distributed in Clade 1. The analysis of the genotypes of multiple isolates from RVVC patients further demonstrated that point mutation and loss of heterozygosity contributed to the microevolution of C. albicans.
Subject(s)
Candida albicans/genetics , Candidiasis, Vulvovaginal/epidemiology , Genetic Variation , Candidiasis, Vulvovaginal/microbiology , China/epidemiology , Female , Humans , Molecular Epidemiology , PrevalenceABSTRACT
OBJECTIVE: To investigate the protective effect of angiopoietin-1 (Ang-1) from capillary endothelial damage in rats with acute necrotizing pancreatitis (ANP). METHODS: 96 male Sprague-Dawley rats were randomly averaged and divided into control group, ANP group, Si-Ang-1 group, and COMP (cartilage oligomeric matrix protein)-Ang-1 group. Animals were killed at 6, 12, and 24 hours after molding. Levels of serum amylase, porcine endothelin 1, C-reactive protein, and Ang-1 were detected; histopathological changes in the pancreas were observed; capillary permeability and Ang-1 expression of the pancreatic tissue were detected by Evans Blue extravasation assay, immunohistochemistry, Western blot, and quantitative polymerase chain reaction. RESULTS: (1) Levels of serum amylase, C-reactive protein, and porcine endothelin-1 increased and level of Ang-1 decrease in the ANP group and Si-Ang-1 group compared with the control group, whereas COMP-Ang-1 group could improve the changes. (2) The order of pancreas pathological changes (mild to severe) is: control group, COMP-Ang-1 group, ANP group, and Si-Ang-1 group. (3) Capillary permeability of the pancreatic tissue in the COMP-Ang-1 group was lower than that in the ANP group. (4) Ang-1 mRNA and protein expression in the COMP-Ang-1 group was significantly higher than in the ANP group. CONCLUSIONS: COMP-Ang-1 can upregulate the expression of Ang-1 protein to promote angiogenesis and improve early inflammatory and pathological damage in ANP group.
Subject(s)
Angiogenesis Inducing Agents/pharmacology , Anti-Inflammatory Agents/pharmacology , Capillary Permeability/drug effects , Pancreas/drug effects , Pancreatitis, Acute Necrotizing/drug therapy , Recombinant Fusion Proteins/pharmacology , Amylases/blood , Angiopoietin-1/blood , Angiopoietin-1/genetics , Animals , C-Reactive Protein/metabolism , Disease Models, Animal , Endothelin-1/blood , Male , Neovascularization, Physiologic , Pancreas/blood supply , Pancreas/metabolism , Pancreas/pathology , Pancreatitis, Acute Necrotizing/blood , Pancreatitis, Acute Necrotizing/genetics , Pancreatitis, Acute Necrotizing/pathology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats, Sprague-Dawley , Time Factors , Up-RegulationABSTRACT
OBJECTIVES: The objectives of this study were to investigate the expression of aquaporin 1 in capillary endothelial cells of rats with experimental acute necrotizing pancreatitis (ANP) and to explore its pathogenic role in capillary leak. METHODS: Sixty-four male Sprague-Dawley rats were randomly divided into control (n = 32) and ANP groups (n = 32). Eight rats in each group were killed at 3, 6, 12, and 18 hours after induction of experimental models. Quantity of ascites and levels of serum amylases were measured. Capillary permeability in pancreas, lung, and intestinal tissue was detected by Evans blue extravasation method. Aquaporin 1 expression in pancreas, lung, and intestinal tissue was detected by real-time polymerase chain reaction, immunohistochemical staining, and Western blot. RESULTS: Serum amylase level was significantly higher in ANP group than in controls (P < 0.05). Evans blue concentration in tissues in the ANP group was significantly higher than that in controls (P < 0.05). Aquaporin 1 mRNA and protein expressions in tissues were significantly less in the ANP group than in the controls (P < 0.05). CONCLUSIONS: The expression of aquaporin 1 was down-regulated in the pancreas, lung, and intestinal tissue of ANP rats, which could play an important role in the pathogenesis of capillary leak syndrome.
