ABSTRACT
OBJECTIVE: To evaluate the effect of in vitro maturation (IVM) of oocytes on the serum cholesterol profile, glucose and insulin tolerance, blood pressure, and heart rate of adult mouse offspring. STUDY DESIGN: The serum cholesterol profile, glucose and insulin tolerance, blood pressure, and heart rate of adult offspring born from in vitro matured oocytes were compared with those of adult offspring born from in vivo matured oocytes. RESULTS: Offspring born from in vitro matured oocytes showed a normal serum cholesterol profile. In the glucose tolerance test, glucose levels were consistently elevated in adult offspring born from in vitro matured oocytes compared with those born from in vivo matured oocytes, but the differences did not reach statistical significance. There were no significant differences in insulin tolerance, blood pressure, and heart rate between adult offspring born from in vitro matured oocytes and those from in vivo matured oocytes. CONCLUSION: No alterations were found in the metabolism profile of adult mouse offspring born from in vitro matured oocytes compared with that from in vivo matured oocytes.
Subject(s)
Fertilization in Vitro/methods , In Vitro Oocyte Maturation Techniques , Metabolic Syndrome/etiology , Oocytes/growth & development , Animals , Blood Glucose/analysis , Blood Pressure , Cells, Cultured , Cholesterol/blood , Embryo Transfer , Female , Glucose Tolerance Test , Heart Rate , Insulin/blood , Lipids/blood , Male , Metabolic Syndrome/blood , Mice , Mice, Inbred C57BL , Risk Factors , Sex FactorsABSTRACT
OBJECTIVE: To evaluate the effect of vitrification of mouse oocytes on the behavior of adult offspring. STUDY DESIGN: Oocytes from mice were vitrified, warmed and inseminated, and two-cell embryos were transferred to foster mothers. The behavioral characterization of the offspring was detected by the Morris water maze test, forced swimming test, and elevated plus maze test, and compared to that of offspring from fresh oocytes. RESULTS: Offspring produced by vitrified oocytes showed normal motor function. In the Morris water maze test of spatial learning there was a slightly decreased time spent in the quadrant containing the platform relative to mice from fresh oocytes, but the difference did not reach statistical significance. In addition, offspring from vitrified oocytes did not exhibit alterations in emotional behavior. CONCLUSION: No alterations were found in the behavioral characterization of adult offspring from vitrified oocytes compared with those from fresh oocytes.
