Improving insulin resistance by sulforaphane via activating the Bacteroides and Lactobacillus SCFAs-GPR-GLP1 signal axis.

Background: Insulin resistance (IR) is closely associated with non-alcoholic fatty liver disease (NAFLD), and the gut microbiome contributes to the development of NAFLD. Sulforaphane (SFN) is a phytochemical in cruciferous vegetables that could improve lipid metabolism disorder. However, whether SFN can alleviate IR in NAFLD by regulating the intestinal flora remains unclear. Methods: SFN was administered to high fat diet (HFD)-fed Wistar rats for 10 weeks. Gut microbiota was analysed by 16S rRNA sequencing and the short chain fatty acids (SCFAs) by gas chromatography. The expression of tight junction protein and the numbers of Lactobacillus, Bacteroides and Bifidobacterium were determined by qPCR. The expression of G-protein-coupled receptor 41/43 (GPR41/43) was determined by western blot. A randomized controlled trial (RCT) was conducted in NAFLD patients with broccoli seed tablets (rich in SFN, 42 mg d-1) as intervention for 12 weeks. Thirty-six volunteers with abnormal glucose before the broccoli seed tablet treatment were selected in the intervention group to analyze their blood glucose, insulin, homeostasis model assessment-insulin resistance index (HOMA-IRI), homeostasis model assessment-insulin sensitivity index (HOMA-ISI) and glucagon-like peptide (GLP-1). Results: SFN reduced blood glucose and HOMA-IRI while increasing insulin sensitivity in HFD rats. SFN reduced glycogen synthase kinase 3 (GSK-3), phosphoenolpyruvate carboxykinase (PEPCK) activity, and phosphorylation of serine residues of IRS-2 induced by HFD. SFN reshaped the gut microbiota composition of HFD-induced rats and, especially, increased the content of Bacteroidaceae, Lactobacillaceae and Bifidobacteriaceae, which are related to the improvement from SFN of the blood glucose and HOMA-IRI. The increased numbers of Bacteroides and Lactobacillus were the targets of SFN to enhance the expression of tight junction proteins ZO-1 and occludin, thereby lowering lipopolysaccharide content to reduce inflammation, ultimately alleviating IR. Bacteroides and Lactobacillus produced SCFAs, which activated GPR41/43 to secrete GLP1. Moreover, it was also confirmed in RCT that SFN intervention increased the level of GLP1 in NAFLD patients, which was positively correlated with the reduction of blood glucose and HOMA-IR. Conclusions: SFN alleviated IR in NAFLD via the Bacteroides and Lactobacillus SCFAs-GPR41/43-GLP1 axis and protected the intestinal mucosal barrier to decrease inflammation.

Pressure-Induced Enhancement in Chemical Looping Reforming of CH4 : A Thermodynamic Analysis with Fe-Based Oxygen Carriers.

Chemical looping reforming of methane (CLRM) with Fe-based oxygen carriers is widely acknowledged as an environmentally friendly and cost-effective approach for syngas production, however, sintering-caused deactivate of oxygen carriers at elevated temperatures of above 900 °C is a longstanding issue restricting the development of CLRM. Here, in order to reduce the reaction temperature without compromising the chemical-looping CH4 conversion efficiency, we proposed a novel operation scheme of CLRM by manipulating the reaction pressure to shift the equilibrium of CH4 partial oxidation towards the forward direction based on the Le Chatelier's principle. The results from thermodynamic simulations showed that, at a fixed reaction temperature, the reduction in pressure led to the increase in CH4 conversion, H2 and CO selectivity, as well as carbon deposition rate of all investigated oxygen carriers. The pressure-negative CLRM with Fe3O4, Fe2O3 and MgFe2O4 could reduce the reaction temperature to below 700 ℃ on the premise of a satisfactory CLRM performance. In a comprehensive consideration of the CLRM performance, energy consumption, and CH4 requirement, NiFe2O4 was the Fe-based OCs best available for pressure-negative CLRM. This study offered a new strategy to address sintering-caused deactivation of materials in chemical looping from the reaction thermodynamics point of view.

An ultrahigh sensitivity acoustic sensor system for weak signal detection based on an ultrahigh-Q CaF2 resonator.

Acoustic sensors with ultrahigh sensitivity, broadband response, and high resolution are essential for high-precision nondestructive weak signal detection technology. In this paper, based on the size effect of an ultrahigh-quality (Q) calcium fluoride (CaF2) resonator, a weak acoustic signal is detected by the dispersive response regime in which an acoustic, elastic wave modulates the geometry and is converted to a resonance frequency shift. Through the structural design of the resonator, the sensitivity reaches 11.54 V/Pa at 10 kHz in the experiment. To our knowledge, the result is higher than that of other optical resonator acoustic sensors. We further detected a weak signal as low as 9.4 µPa/Hz1/2, which greatly improved the detection resolution. With a good directionality of 36.4 dB and a broadband frequency response range of 20 Hz-20 kHz, the CaF2 resonator acoustic sensing system can not only acquire and reconstruct speech signals over a long distance but also accurately identify and separate multiple voices in noisy environments. This system shows high performance in weak sound detection, sound source localization, sleep monitoring, and many other voice interaction applications.

Dietary Isothiocyanates: Novel Insights into the Potential for Cancer Prevention and Therapy.

Diet plays an important role in health. A high intake of plant chemicals such as glucosinolates/isothiocyanates can promote optimal health and decrease the risk of cancer. Recent research has discovered more novel mechanisms of action for the effects of isothiocyanates including the modulation of tumor microenvironment, the inhibition of the self-renewal of stem cells, the rearrangement of multiple pathways of energy metabolism, the modulation of microbiota, and protection against Helicobacter pylori. However, the hormetic/biphasic effects of isothiocyanates may make the recommendations complicated. Isothiocyanates possess potent anti-cancer activities based on up-to-date evidence from in vitro and in vivo studies. The nature of hormesis suggests that the benefits or risks of isothiocyanates largely depend on the dose and endpoint of interest. Isothiocyanates are a promising class of cancer-preventative phytochemicals, but researchers should be aware of the potential adverse (and hormetic) effects. In the authors' opinion, dietary isothiocyanates are better used as adjunctive treatments in combination with known anti-cancer drugs. The application of nano-formulations and the delivery of isothiocyanates are also discussed in this review.

Insight into the adsorption isotherms and kinetics of Pb (II) on pellet biochar via in-situ non-destructive 3D visualization using micro-computed tomography.

The micro-CT technique was applied in adsorption visualization of Pb (II) on the pellet biochar derived from wheat straw to provide information on understanding the complex heavy metal-biochar interaction during the process. The 3D distribution of Pb (II) on the biochar was well in line with the results of isothermal and kinetic adsorption experiments as well as those of simulation with Langmuir and Weber-Morris intraparticle diffusion (IPD) models. It was shown that Pb (II) was preferentially adsorbed on the surface of the biochar at an initial Pb (II) concentration of 50 mg/L. However, at a higher initial concentration of 100 mg/L, the adsorption process occurred in a two-stage regime, namely rapid surface adsorption followed by slow intraparticle diffusion. This research offered a new way for investigation of the complex adsorption behavior of heavy metals on biochar, as well as construction and optimization of related adsorption models.

Decarbonising the iron and steel sector for a 2 °C target using inherent waste streams.

The decarbonisation of the iron and steel industry, contributing approximately 8% of current global anthropogenic CO2 emissions, is challenged by the persistently growing global steel demand and limitations of techno-economically feasible options for low-carbon steelmaking. Here we explore the inherent potential of recovering energy and re-using materials from waste streams, high-temperature slag, and re-investing the revenues for carbon capture and storage. In a pathway based on energy recovery and resource recycling of glassy blast furnace slag and crystalline steel slag, we show that a reduction of 28.5 ± 5.7% CO2 emissions to the sectoral 2 °C target requirements in the iron and steel industry could be realized in 2050 under strong decarbonization policy consistent with low warming targets. The technological schemes applied to engineer this high-potential pathway could generate a revenue of US$35 ± 16 and US$40 ± 18 billion globally in 2035 and 2050, respectively. If this revenue is used for carbon capture and storage implementation, equivalent CO2 emission to the 2 °C sectoral target requirements is expected to be reduced before 2050, without any external investments.

Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).

SCOPE: Mitochondria-associated membrane (MAM) connects endoplasmic reticulum (ER) and mitochondria plays a significant role in lipid metabolism and Ca2+ homeostasis. Albeit sulforaphane (SFN) shows potential in ameliorating excessive fat accumulation and mitochondrial function; whether MAM is a target of SFN and its underlying mechanisms are still unclear. METHODS AND RESULTS: High-fat-intake models are established both in vivo and in vitro. SFN widens the distance between ER and mitochondria and down-regulates MAM tether protein mitofusin-2. SFN reverses the increase of Ca2+ induced by fatty acid and inhibits the Ca2+ channel inositol-1,4,5-trisphosphate receptor (IP3R). Compared with high fat group, SFN alleviates Ca2+ overload in the mitochondria and suppresses mitochondrial calcium uniporter (MCU). Furthermore, SFN increases mitochondrial DNA quantities and mitochondria membrane potential, while decreasing reactive oxygen species (ROS) production. Finally, SFN increases mitochondria complexes IV content and ATP synthesis. CONCLUSION: These results suggest that SFN balances the Ca2+ homeostasis in the MAM through regulating Ca2+ flux by Ca2+ channel IP3R and MCU.

Optimization of VCSEL photon lifetime for minimum energy consumption at varying bit rates.

Optimum photon lifetimes of vertical-cavity surface-emitting lasers (VCSELs) are shown to be target bit rate dependent. A comparison of the power budget in fJ/bit for optimized VCSELs operating at 25 Gb/s and 50 Gb/s is given. At 25 Gbit/s, the energy per bit ratio is lower than 100 fJ/bit presenting a 75% reduction as compared to the 50 Gb/s values. The cavity dip / gain peak detuning for maximizing temperature stability must be similarly adjusted to the bit rate as shown here. These conclusions are valid for any VCSELs, e.g. those emitting at 850 nm, 880 nm, 910 nm, 940 nm or 980 nm, presently investigated by us.

High power femtosecond semiconductor lasers based on saw-toothed master-oscillator power-amplifier system with compressed ASE.

High power femtosecond semiconductor laser based on saw-toothed taper mode-locked laser and amplifier was demonstrated with compressed amplified spontaneous emission (ASE). The external-cavity mode-locked taper laser generated the clean optical pulses without any sub-pulse components. A semiconductor optical amplifier (SOA) with tilted taper waveguide and saw-toothed edge reduced evidently the ASE background. The saw-tooth microstructures were optimized and it was found that the saw-tooth of right-right angled triangle showed the best effect. The ratio of the maximum intensity to background radiation was increased by 21.9% and the power was increased by 30.5% due to the saw-tooth microstructure in the SOA. The pulse duration of 495 fs and a peak power over 1.5 kW with repetition rate of 579 MHz were realized after a double-pass grating compressor.

Extracting more light for vertical emission: high power continuous wave operation of 1.3-µm quantum-dot photonic-crystal surface-emitting laser based on a flat band.

For long distance optical interconnects, 1.3-µm surface-emitting lasers are key devices. However, the low output power of several milliwatts limits their application. In this study, by introducing a two-dimensional photonic-crystal and using InAs quantum dots as active materials, a continuous-wave, 13.3-mW output power, 1.3-µm wavelength, room-temperature surface-emitting laser is achieved. In addition, such a device can be operated at high temperatures of up to 90 °C. The enhanced output power results from the flat band structure of the photonic crystal and an extra feedback mechanism. Surface emission is realized by photonic crystal diffraction and thus the distributed Bragg reflector is eliminated. The proposed device provides a means to overcome the limitations of low-power 1.3-µm surface-emitting lasers and increase the number of applications thereof.

Targeting PLIN2/PLIN5-PPARγ: Sulforaphane Disturbs the Maturation of Lipid Droplets.

SCOPE: The effects of sulforaphane (SFN) on the maturation of lipid droplets (LDs)-the storage units for free fatty acids and sterols as triacylglycerides (TAG) and cholesterol esters (CE)-are far from being understood, despite the fact that SFN is known to be beneficial for ameliorating lipid metabolism disorders. METHODS AND RESULTS: High-fat-intake models are established in both HHL-5 hepatocytes and rodents. The numbers and sizes of LDs are decreased by SFN. The accumulation of lipid core components (TAG & CE) is reduced and the expression of their key synthetases, acyl-coenzyme A: diacylglycerol acyltransferases 2 (DGAT2) and acyl-coenzyme A: cholesterol acyltransferases 1 (ACAT1), is also inhibited. Moreover, SFN decreases LD-associated protein PLIN2 and PLIN5 expression, but not that of PLIN1 and PLIN3, both in vivo and in vitro. Furthermore, over-expression of peroxisome proliferator-activated receptor gamma (PPARγ) induces the accumulation of TAG and the up-regulation of PLIN2 and PLIN5, which are not reversed by SFN. These results suggest that PPARγ may be a target of SFN in lipid metabolism. CONCLUSION: SFN disturbs LD maturation by inhibiting the formation of the neutral lipid core and decreases PLIN2 and PLIN5 via down-regulation of PPARγ.

Loss tailoring of high-power broad-area diode lasers.

Broad-area diode lasers (BALs) with high power are highly desirable for a variety of applications. However, such lasers suffer from strongly deteriorated beam quality due to multimode behavior in the lateral direction. In this Letter, we present an approach to flexibly tailor the optical loss of different-order lateral modes by etching micro-holes on the laser mesa with controlled position and numbers. Through arranging the micro-holes at the peak positions of high-order lateral modes with an increasing number from the mesa center to both edges, high-order modes are suppressed due to a larger propagation loss than the fundamental mode. As a result of enhanced mode discrimination, we demonstrate that this technique provides a greatly improved beam quality and about two times higher brightness for 100 µm wide BAL, while maintaining high power and slope efficiency output.

Calcium-looping reforming of methane realizes in situ CO2 utilization with improved energy efficiency.

Closing the anthropogenic carbon cycle is one important strategy to combat climate change, and requires the chemistry to effectively combine CO2 capture with its conversion. Here, we propose a novel in situ CO2 utilization concept, calcium-looping reforming of methane, to realize the capture and conversion of CO2 in one integrated chemical process. This process couples the calcium-looping CO2 capture and the CH4 dry reforming reactions in the CaO-Ni bifunctional sorbent-catalyst, where the CO2 captured by CaO is reduced in situ by CH4 to CO, a reaction catalyzed by catalyzed by the adjacent metallic Ni. The process coupling scheme exhibits excellent decarbonation kinetics by exploiting Le Chatelier's principle to shift reaction equilibrium through continuous conversion of CO2, and results in an energy consumption 22% lower than that of conventional CH4 dry reforming for CO2 utilization. The proposed CO2 utilization concept offers a promising option to recycle carbon directly at large CO2 stationary sources in an energy-efficient manner.

Enhancing Third- and Fifth-Order Nonlinearity via Tunneling in Multiple Quantum Dots.

The nonlinearity of semiconductor quantum dots under the condition of low light levels has many important applications. In this study, linear absorption, self-Kerr nonlinearity, fifth-order nonlinearity and cross-Kerr nonlinearity of multiple quantum dots, which are coupled by multiple tunneling, are investigated by using the probability amplitude method. It is found that the linear and nonlinear properties of multiple quantum dots can be modified by the tunneling intensity and energy splitting of the system. Most importantly, it is possible to realize enhanced self-Kerr nonlinearity, fifth-order nonlinearity and cross-Kerr nonlinearity with low linear absorption by choosing suitable parameters for the multiple quantum dots. These results have many potential applications in nonlinear optics and quantum information devices using semiconductor quantum dots.

Parity-time symmetry in coherent asymmetric double quantum wells.

A coherently prepared asymmetric double semiconductor quantum well (QW) is proposed to realize parity-time (PT) symmetry. By appropriately tuning the laser fields and the pertinent QW parameters, PT-symmetric optical potentials are obtained by three different methods. Such a coherent QW system is reconfigurable and controllable, and it can generate new approaches of theoretically and experimentally studying PT-symmetric phenomena.

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis In Vivo and In Vitro.

SCOPE: Sulforaphane (SFN) is reported to reduce the accumulation of lipids. However, the underling mechanism remains unclear. In this study, the potential of SFN to improve lipid metabolism is investigated through altering mitochondrial function and biogenesis-related mechanisms. METHODS AND RESULTS: The abnormal lipid metabolism model was established both in HHL-5 cells and in rats by feeding a high-fat diet (HFD) for 10 weeks. The current findings suggest that SFN alleviates the swelling of mitochondria and stimulates mitochondrial biogenesis. The reduced expression of NRF1 and TFAM, were reversed by SFN. SFN increases the levels of antioxidant compounds via nuclear factor erythroid-2-related factor (Nrf2) activation. Furthermore, SFN improves multiple mitochondrial bioactivities, such as mitochondrial membrane potential, ATP, and the electron transfer chain based on PGC-1α pathway. SFN also activates lipolysis by transcriptionally upregulating adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL). CONCLUSIONS: SFN enhances utilization of lipids via both the PGC- 1α-dependent promotion of mitochondrial biogenesis and Nrf2 dependent improvement of mitochondrial function.

Inherent potential of steelmaking to contribute to decarbonisation targets via industrial carbon capture and storage.

Accounting for ~8% of annual global CO2 emissions, the iron and steel industry is expected to undertake the largest contribution to industrial decarbonisation. Despite the launch of several national and regional programmes for low-carbon steelmaking, the techno-economically feasible options are still lacking. Here, based on the carbon capture and storage (CCS) strategy, we propose a new decarbonisation concept which exploits the inherent potential of the iron and steel industry through calcium-looping lime production. We find that this concept allows steel mills to reach the 2050 decarbonisation target by 2030. Moreover, only this concept is revealed to exhibit a CO2 avoidance cost (12.5-15.8 €2010/t) lower than the projected CO2 trading price in 2020, whilst the other considered options are not expected to be economically feasible until 2030. We conclude that the proposed concept is the best available option for decarbonisation of this industrial sector in the mid- to long-term.

High-brightness diode lasers obtained via off-axis spectral beam combining with selective feedback.

We proposed a modified off-axis spectral beam combining method, based on the concept of selective feedback. A high reflectivity mirror with a fixed width was used to select and couple back the optical modes in the external cavity. The emission power exceeding 20 W with M2 factors of 2.7 × 4.4 in the fast and slow axes was demonstrated. The beam quality of the system was improved by a factor of three to four compared with that of a single emitter, and a high brightness of 190 MW cm-2 str-1 was achieved.

Going beyond the beam quality limit of spectral beam combining of diode lasers in a V-shaped external cavity.

A novel spectral beam combining (SBC) approach based on off-axis feedback in a V-shaped external cavity (VSBC) was proposed and demonstrated. A highly reflecting mirror was used to supply the optical feedback by partial overlapping the beam. The advantages of simple setup, output coupler free, tunable beam quality and emission power over traditional SBC were presented. The beam quality exceeds single emitter with the similar energy conversion efficiency to the traditional SBC. The M2 factors of 2.31 × 3.76 in fast and slow axes and a brightness of 122 MWcm-2sr-1 were realized at 30 A based on a commercial broad-area diode laser array.