ABSTRACT
OBJECTIVE: To establish a mutual mode fingerprint of Curcuma wenyujin for quality control of C. wenyujin. METHODS: Waters Symmetry C18 column was used; the mobile phase was methanol-water in a linear gradient elution with the flow rate of 1.0 mL/ min, the temperature of column was 30 degrees C; the detection wavelength was set at 215 nm. 10 batches of C. wenyujin from different places and different time were determined, 3 batches of C. phaeocaulis and 3 batches of C. kwangsiensis were determined in the same chromatographic conditions. RESULTS: 11 mutual peaks in the fingerprint of the 10 groups of C. wenyujin, and the S peak among them represented germacrone. CONCLUSION: Curcuma wenyujin's fingerprints have strong feature and specificity, which can be combined with assaying in the quality control of C. wenyujin.
Subject(s)
Chromatography, High Pressure Liquid/methods , Curcuma/chemistry , Oils, Volatile/chemistry , Plants, Medicinal/chemistry , Curcuma/classification , Curcuma/growth & development , Oils, Volatile/analysis , Plants, Medicinal/classification , Plants, Medicinal/growth & development , Quality Control , Reproducibility of Results , Rhizome/chemistry , Sesquiterpenes/analysisABSTRACT
Bioassay-guided fractionation, combined with screening based on EGF-responsive neural stem cells (NSCs) differentiation assay, has been used to search for active molecules from Panax notoginseng. Ginsenosides Rg3 (1), Rk1 (2), and Rg5 (3) were identified as potential neurogenic molecules. The degrees of their neurogenic effects were found to be 3 > 2 > 1. The neurogenic effect of 3 represents a biphasic dose- and time-dependent regulation. Transient exposure of NSCs to 8 microM 3 for 24 h followed by 1 microM and 72 h incubation was the optimal procedure for the induction of neurons in NSCs, and compound 3 resulted in an approximately 3-fold increase in neurogenesis at the expense of astrogliogenesis. The neurogenic effect of 3 was completely eliminated by the Ca2+ channel antagonist nifedipine. These findings imply that 3 may be utilized as a pharmacological agent in studying the molecular regulation of neurogenesis of brain stem cells and, subsequently, for treatment of neurodegenerative diseases.
Subject(s)
Drugs, Chinese Herbal/isolation & purification , Drugs, Chinese Herbal/pharmacology , Ginsenosides/isolation & purification , Ginsenosides/pharmacology , Glycosides/isolation & purification , Glycosides/pharmacology , Neurons/drug effects , Panax notoginseng/chemistry , Plants, Medicinal/chemistry , Stem Cells/drug effects , Triterpenes/isolation & purification , Triterpenes/pharmacology , Brain Stem/cytology , Calcium Channel Blockers/pharmacology , Dose-Response Relationship, Drug , Drugs, Chinese Herbal/chemistry , Epidermal Growth Factor/metabolism , Ginsenosides/chemistry , Glycosides/chemistry , Molecular Structure , Neurodegenerative Diseases/etiology , Neurodegenerative Diseases/therapy , Nifedipine/pharmacology , Time Factors , Triterpenes/chemistryABSTRACT
OBJECTIVE: To establish a IE-HPLC method for the determination of trigonelline in the fruit and seed of Quisqualis indica. METHOD: The method was carried out by using Sperisorb SCX column with a mobile phase consisting of 20 mmol x L(-1) phorsphate (pH3.2, titrate a 20 mmol x L(-1) monsodium salt with a 20 mmol x L(-1) solution of phosphoric acid), flow rate of 1.0 mL x min(-1), and the detection at 265 nm. RESULT: By using SCX-modified silica column, trigonelline in Fructus Quisqualis can be baseline separated and determinated. The retention mechanisms proved to be contributed mainly by ion-exchange with the SCX moieties. The validity of methods was demonstrated with respect to linearity, precision, reproducibility and recovery. It was found that there was no trigonelline occurring in the peel of Fructus Quisqualis. CONCLUSION: This assay method can be used for the quality control of Fructus Quisqualis.
