ABSTRACT
Understanding of the transporters involved in the uptake and translocation of agrochemicals in plants could provide an opportunity to guide pesticide to the site of insect feeding. The product of Arabidopsis thaliana gene AtLHT1 makes a major contribution to the uptake into the roots of free amino acids and some of their derivatives. Here, a chlorantraniliprole-glycine conjugate (CAP-Gly-1) was tested for its affinity to AtLHT1 both in planta and in vitro. Seedlings deficient in AtLHT1 exhibited a reduction with respect to both the uptake and root-to-shoot transfer of CAP-Gly-1; plants in which AtLHT1 was constitutively expressed were more effective than wild type in term of their root uptake of CAP-Gly-1. Protoplast patch clamping showed that the presence in the external medium of CAP-Gly-1 was able to induce AtLHT1 genotype-dependent inward currents. An electrophysiology-based experiment carried out in Xenopus laevis oocytes expressing AtLHT1 showed that AtLHT1 had a high in vitro affinity for CAP-Gly-1. The observations supported the possibility of exploiting AtLHT1 as a critical component of a novel delivery system for amino acid-based pesticide conjugates.
Subject(s)
Amino Acid Transport Systems, Basic/metabolism , Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Glycine/metabolism , Insecticides/metabolism , ortho-Aminobenzoates/metabolism , Amino Acid Transport Systems, Basic/genetics , Animals , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Biological Transport , Glycine/chemistry , Insecticides/chemistry , Oocytes/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plant Shoots/genetics , Plant Shoots/metabolism , Xenopus laevis , ortho-Aminobenzoates/chemistryABSTRACT
A chemical investigation of the whole plant of Pronephrium megacuspe (Bak.) Holtt. led to the isolation of four flavonoids, pronephrones A-D (1-4), which were firstly reported. The chemical structures of four compounds were established using spectroscopic methods. These isolates were further evaluated for cytotoxicity to ovarian cells of Spodoptera litura Fabricius.
Subject(s)
Cytotoxins/isolation & purification , Drugs, Chinese Herbal/isolation & purification , Flavonoids/isolation & purification , Plants, Medicinal/chemistry , Animals , Cytotoxins/chemistry , Cytotoxins/pharmacology , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/pharmacology , Female , Flavonoids/chemistry , Flavonoids/pharmacology , Houseflies , Insecticides/chemistry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Ovary/cytology , Ovary/drug effects , Spodoptera/cytology , Spodoptera/drug effectsABSTRACT
A series of tetraethynylsilanes (TETS) have been synthesized by reaction of silicon tetrachloride (SiCl(4)) with Ar-C triple bond CLi, which was prepared in situ by treatment of Ar-C triple bond CH with n-BuLi. For these TETS thus prepared, their photoactivated insecticidal activities against the 4th-instar larvae of Aedes albopictus (Skuse) were evaluated to enrich the structure-activity relationship. In particular, compound 8 exhibited excellent photoactivated insecticidal activity, the LC(50) value was 0.1346 mg L(-1) under UV light treatment and the irradiation-generated enhancement in the activity was more than 69.58-fold, thus could be exploitable as ideal analog candidates in the search for new photoactivated insecticide leads.
