ABSTRACT
According to the status of the 2015 edition of Chinese Pharmacopoeia and following the idea of establishing the most rigorous standard, to provide constructive suggestions for the revision of the 2020 edition of Chinese Pharmacopoeia, three suggestions are put forward to the current compilation work as follows: the name about multi-sourced traditional Chinese medicine; Non-standard traditional Chinese medicine entered the prescription of Chinese medicine in Chinese Pharmacopoeia; The font and order of words standardization of Latin scientific name of traditional Chinese medicine.
ABSTRACT
The gene encoding Trichosanthes kirilowii defensin (TDEF1) was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). The newly discovered TDEF1 cDNA contains 231 bp (Genbank accession number DQ526373) and encodes a 76-amino acid protein, which consists of a 29-amino acid signal peptide and a 47-amino acid mature peptide. The partial cDNA, corresponding to the mature peptide coding region of TDEF1, was inserted into bacterial expression vector pET32a(+). Subsequent expression showed that TDEF1 was produced as a 26-kDa fusion protein in the form of inclusion body in Escherichia coli BL21 (DE3). After protein refolding and purification, the fusion TDEF1 displayed an inhibitive activity against the fungal pathogen, Fusarium oxysporum, with EC(50) of 247 microg/ml by means of fungal growth inhibition method.
Subject(s)
Antifungal Agents/pharmacology , Defensins/genetics , Defensins/metabolism , Escherichia coli/metabolism , Plant Leaves/genetics , Recombinant Fusion Proteins/metabolism , Trichosanthes/genetics , Base Sequence , Defensins/chemistry , Escherichia coli/genetics , Fusarium/drug effects , Microbial Sensitivity Tests , Molecular Sequence Data , Plant Leaves/chemistry , Plant Leaves/metabolism , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Sequence Analysis, DNA , Trichosanthes/chemistry , Trichosanthes/metabolismABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of ethanolic extract from herbs of Dicliptera chinensis.</p><p><b>METHOD</b>The compounds were separated by silica gel column chromatography, preparation TLC and reverse phase HPLC, their structures were identified by the spectroscopic methods of UV, NMR and MS.</p><p><b>RESULT</b>Seven compounds were isolated from ethyl acetate extract. Their structure were identified as octasulphur (1), secoisolariciresinol dimethyl ether diacetate (2), 5-methoxy-4, 4'-di-O-methyl secolariciresinol (3), chinensinaphthol methyl ester (4), loliolide (5), beta-sitosterol 3-O-beta-D-glucopyranoside (6) and stigmasterol 3-O-beta-D-glucopyranoside (7).</p><p><b>CONCLUSION</b>All the compounds except 6 were obtained from the plants of Dicliptera for the first time.</p>
Subject(s)
Acanthaceae , Chemistry , Benzofurans , Chemistry , Glucosides , Chemistry , Plants, Medicinal , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for determination of protopine and isocorydine in root of Dactylicapnos scandens.</p><p><b>METHOD</b>The separation was performed in a PHENOMENEX-C18 column with a mobile phase of methanol-0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine)(50:50), The detection wavelength was at 254 nm and the flow rate was 0.8 mL x min(-1).</p><p><b>RESULT</b>The average recovery of Protopine and Isocorydine was 97.9%, 98.6% respectively, and RSD 1.3%, 1.4%.</p><p><b>CONCLUSION</b>This method is accurate, simple and reliable. It can be used for quality control of D. scandens.</p>
Subject(s)
Aporphines , Benzophenanthridines , Berberine Alkaloids , Chromatography, High Pressure Liquid , Papaveraceae , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Quality ControlABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Kangning granules on inflammatory and immunology.</p><p><b>METHOD</b>Pedal swelling induced by 1% agar in mice, dermal capillary hyper-permeability induced by histamine in rats were used to evaluate the anti-inflammatory of Kangning granules, and the clearance rate of intravenous charcoal particles was used to study its effect on non-specific immunologic functions. Serum haemolysin level and the number of antibody secretory cells in spleen were determined to assess its effects of specific immunologic functions. MTT colored method was used to determine the effect of Kangning granules on T- or B-Lymphocytes proliferation.</p><p><b>RESULT</b>5, 10, 20 g x kg(-1) of Kangning Granules significantly inhibited pedal swelling induced by agar in mice (P < 0.05, P < 0.01). 2.4, 4.8, 9.6 g x kg(-1) of Kangning Granules evidently decreased dermal capillary hyper-permeability induced by histamine in rats (P < 0.05, P < 0.01). 10, 20 g x kg(-1) of Kangning Granules enhanced the phagocytic function and phagocytosing velocity in mice (P < 0.05). 5, 10, 20 g x kg(-1) of Kangning Granules obviously increased serum haemolysin level and the number of antibody secretory cells in spleen (P < 0.05, P < 0.01). 20 g x kg(-1) of Kangning Granules promoted B-Lymphocytes proliferation (P < 0.05).</p><p><b>CONCLUSION</b>Kangning granules significantly inhibited inflammatory reaction and increase immune functions in animals.</p>
Subject(s)
Animals , Female , Male , Mice , Rats , Anti-Inflammatory Agents, Non-Steroidal , Pharmacokinetics , Pharmacology , Capillary Permeability , Drug Combinations , Drugs, Chinese Herbal , Pharmacokinetics , Pharmacology , Echinacea , Chemistry , Edema , Pathology , Foot Diseases , Pathology , Immunologic Factors , Pharmacokinetics , Pharmacology , Lymphocyte Activation , Mice, Inbred BALB C , Phagocytosis , Plants, Medicinal , Chemistry , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To offer evidences for quality control of medicinal plant of Dactylicapnos scandens.</p><p><b>METHOD</b>Pharmacognostic studies were carried out through field collection, morphological and microscopic characteristics, and TLC.</p><p><b>RESULT</b>Observation and description of the experimentation were made.</p><p><b>CONCLUSION</b>Dactylicapnos scandens can be identified from Genus of Dactylicapnos which has the similar morphological characteristics. The morphological and microscopic characteristics and the results of TLC can be used as evidences for quality control of this medicinal plant.</p>
Subject(s)
Chromatography, Thin Layer , Papaveraceae , Chemistry , Pharmacognosy , Plant Leaves , Plant Roots , Plant Stems , Plants, Medicinal , Chemistry , Quality ControlABSTRACT
<p><b>OBJECTIVE</b>To study the lipid constituents from Echinacea purpurea.</p><p><b>METHOD</b>The compounds were isolated by chromatography method and the structures were identified on the basis of spectral analyses.</p><p><b>RESULT</b>Five compounds were isolated and identified as, 1 beta, 6 alpha-dihydroxy-4(14)-eudesmene(1), (2E, 4E, 8Z, 10E)-N-isobutyl-2,4,8,10-dodecatetraenamide(2), (2E, 4E, 8Z, 10Z)-N-isobutyl-2,4,8,10-dodecatetraenamide(3), cerotic acid(4), hyxacosyl alcohol(5).</p><p><b>CONCLUSION</b>Compounds 1,4 and 5 were obtained from the plant for the first time.</p>
Subject(s)
Echinacea , Chemistry , Fatty Acids , Chemistry , Fatty Alcohols , Chemistry , Plants, Medicinal , Chemistry , Sesquiterpenes , ChemistryABSTRACT
<p><b>AIM</b>To study the water-soluble constituents of Echinacea purpurea.</p><p><b>METHODS</b>The compounds were isolated by chromatography method and their structures were identified on the basis of spectral analyses.</p><p><b>RESULTS</b>Five compounds were identified as 2-(4-hydroxylphenyl)-ethyl-O-alpha-L-rhamnopy-ranoasyl-(1-->6)-beta-D- glucopyranoside, named echipuroside A 1); (6S, 9R)-6-hydroxy-3-one-alpha-inonol-9-O-beta-D-glucopyranoside 2), ampelopsisionoside 3); phenylmethyl-6-O-beta-D-xylopyranosyl(1-->6)-beta-D-glucopyranoside 4) and phyenylmethyl-beta-D-glucopyranoside 5).</p><p><b>CONCLUSION</b>Compound 1 is a new compound, the others were isolated from this plant for the first time.</p>
