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Biochem Biophys Res Commun ; 121(1): 47-54, 1984 May 31.
Article in English | MEDLINE | ID: mdl-6375669

ABSTRACT

Reaction centers from Rhodopseudomonas sphaeroides R 26 have been isolated from a crude extract obtained by lauryldimethylamine oxide extraction of chromatophore membranes, by HPLC using a combination of surface-mediated and size exclusion chromatography. The eluted RCs exhibit a normal activity (t 1/2 of the back-reaction is 70 ms) and are recovered in good yield (over 50% based on the activity) and purity (based on the A 280 nm/A 800 nm = 1.30 +/- 0.05 ratio and the characteristic 3 polypeptides SDS-PAGE pattern). The elution time (5-10 mn) is about two orders of magnitude faster than for the classical purification techniques.


Subject(s)
Bacterial Proteins/isolation & purification , Membrane Proteins/isolation & purification , Rhodobacter sphaeroides/analysis , Chromatography, High Pressure Liquid , Detergents , Dimethylamines , Hydrogen-Ion Concentration , Osmolar Concentration , Photochemistry , Photosynthetic Reaction Center Complex Proteins
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