ABSTRACT
Aging and neurodegeneration entail diverse cellular and molecular hallmarks. Here, we studied the effects of aging on the transcriptome, translatome, and multiple layers of the proteome in the brain of a short-lived killifish. We reveal that aging causes widespread reduction of proteins enriched in basic amino acids that is independent of mRNA regulation, and it is not due to impaired proteasome activity. Instead, we identify a cascade of events where aberrant translation pausing leads to reduced ribosome availability resulting in proteome remodeling independently of transcriptional regulation. Our research uncovers a vulnerable point in the aging brain's biology - the biogenesis of basic DNA/RNA binding proteins. This vulnerability may represent a unifying principle that connects various aging hallmarks, encompassing genome integrity and the biosynthesis of macromolecules.
ABSTRACT
Robots start to play a role in our social landscape, and they are progressively becoming responsive, both physically and socially. It begs the question of how humans react to and interact with robots in a coordinated manner and what the neural underpinnings of such behavior are. This exploratory study aims to understand the differences in human-human and human-robot interactions at a behavioral level and from a neurophysiological perspective. For this purpose, we adapted a collaborative dynamical paradigm from the literature. We asked 12 participants to hold two corners of a tablet while collaboratively guiding a ball around a circular track either with another participant or a robot. In irregular intervals, the ball was perturbed outward creating an artificial error in the behavior, which required corrective measures to return to the circular track again. Concurrently, we recorded electroencephalography (EEG). In the behavioral data, we found an increased velocity and positional error of the ball from the track in the human-human condition vs. human-robot condition. For the EEG data, we computed event-related potentials. We found a significant difference between human and robot partners driven by significant clusters at fronto-central electrodes. The amplitudes were stronger with a robot partner, suggesting a different neural processing. All in all, our exploratory study suggests that coordinating with robots affects action monitoring related processing. In the investigated paradigm, human participants treat errors during human-robot interaction differently from those made during interactions with other humans. These results can improve communication between humans and robot with the use of neural activity in real-time.
