1.
Gene
; 168(1): 67-72, 1996 Feb 02.
Article
in English
| MEDLINE
| ID: mdl-8626067
ABSTRACT
We explored transposition in Streptomyces fradiae (Sf) as a means to insert a second copy of the tylF gene to improve tylosin (Ty) production. Transposons Tn5096 and Tn5099 transposed relatively randomly in Sf, and many of the insertions caused no deleterious effects on Ty production yields. Tn5098, a derivative of Tn5096 containing tylF and tylJ genes, recombined into the chromosome into the tyl gene cluster and transposition was not observed. However, following the tagging of a neutral site (NS) by Tn5099 transposition, tylF was effectively inserted into the NS by homologous recombination (transposon exchange). Recombinants obtained by transposon exchange produced higher yields of Ty.
Subject(s)
DNA Transposable Elements/genetics , Mutagenesis, Insertional/genetics , Streptomyces/metabolism , Tylosin/biosynthesis , Anti-Bacterial Agents/biosynthesis , Blotting, Southern , DNA Probes , Gene Dosage , Leucomycins/biosynthesis , Plasmids/genetics , Recombination, Genetic/genetics , Streptomyces/genetics
2.
J Org Chem
; 39(15): 2291-2, 1974 Jul 26.
Article
in English
| MEDLINE
| ID: mdl-4854129