Subject(s)
Lymphoma/microbiology , Animals , Disease Models, Animal , Herpesviridae , Lymphoma/pathology , Primates , Rabbits , RetroviridaeABSTRACT
The possibility of complementation between herpesvirus papio (HVP) and Epstein-Barr virus (EBV) was investigated. Strain 594S-F9 of HVP, unlike strain P3HR-1 of EBV, is not capable of inducing virus antigen synthesis in the EBV genome-carrying, nonproducer lymphoma cell line Raji. The effects of dual infection with these viruses were studied. With untreated viruses, the percentage of cells positive for viral antigens was equal to or slightly less than that in cultures infected with P3HR-1 virus only. However, if UV-irradiated P3HR-1 virus was employed in the dual infection, the relative number of virus antigen-positive cells was enhanced over cultures infected with P3HR-1 virus alone. These results suggest functional complementation between EBV and HVP.
Subject(s)
Genes, Viral , Herpesviridae/genetics , Herpesvirus 4, Human/genetics , Animals , Antigens, Viral , Burkitt Lymphoma , Cell Line , Genetic Complementation Test , Herpesviridae/physiology , Herpesvirus 4, Human/physiology , Humans , PapioABSTRACT
Ways of lymphotropic baboon herpesvirus (HVP) secretion and its excretion into the environment were investigated. Oral swabs and feces from the Sukhumi main stock hamadryas baboons characterized by a high risk for malignant lymphoma and the baboon stock living in isolation in the forest were used as materials for the investigations. Macaque groups of the Sukhumi stock were used as controls. It could be shown that the HVP was resistent in the oral cavity of the main stock baboons and was isolated from oral swabs of these animals both from those with malignant lymphoma and clinically healthy individuals. No virus was isolated from feces of these animals. The virus could not be isolated from oral swabs of the isolated baboon stock and macaques.
Subject(s)
Herpesviridae/isolation & purification , Lymphoma/veterinary , Monkey Diseases/microbiology , Mouth/microbiology , Papio/microbiology , Animals , Cell Line , Feces/microbiology , Herpesviridae/growth & development , Lymphoma/microbiology , Macaca/microbiologyABSTRACT
Isolation of the Epstein-Barr virus (EBV) in suspension lymphoblastoid cell lines from human patients with tumor diseases, mainly malignant lymphoma, has been described. It has been shown that the EBV was isolated from human patients with myeloid type of leukemia in 75% of cases. A similar virus was also isolated from patients with Hodgkin's disease and leukemoid reaction of the myeloid type for lung cancer. Morphological, cytochemical, immunological, and cytogenetic characteristics of the cell lines in which the EBV is replicated have been investigated.
Subject(s)
Herpesvirus 4, Human/isolation & purification , Leukemia/microbiology , Virus Cultivation , Cell Line , Culture Techniques , Herpesvirus 4, Human/ultrastructure , Histocytochemistry , Hodgkin Disease/blood , Hodgkin Disease/microbiology , Humans , Karyotyping , Leukemia/blood , Leukocytes/ultrastructure , Microscopy, ElectronABSTRACT
A new technique (indirect double immunodiffusion) for detection of EBV-associated soluble antigen and corresponding antibodies has been developed. This technique includes three steps: 1) simple double immunodiffusion with extracts of Raji cells (or other EBV-genome positive cells) and human sera containing antibodies against EBV-associated soluble antigen; 2) extensive washing and treatment with anti-human globulin; 3) extensive washing and treatment with tannic acid. Using this test it was shown that the soluble antigen indistinguishable from EBV-associated soluble antigen was present in KMPG-1 cells producing HVP.
Subject(s)
Antigens, Viral , Herpesviridae/immunology , Animals , Cell Line , Haplorhini , Herpesvirus 4, Human/immunology , PapioABSTRACT
Two continuous suspension lymphoblastoid cell cultures designated as SPG-1 and KMPG-1 have been established from the bone marrow and spleen cells of the hamadryas baboon no. 9386 with malignant lymphoma. Virus particles morphologically characterized as herpes-like have been revealed in KMPH-1 and SPG-1 cultures in 2-10% of cells. The paper describes the establishment of these suspension cultures.
