ABSTRACT
AIM: To evaluate the presence of the aggregated proteins in malignant and benign neoplasms for clarifying the role of impaired protein metabolism in the formation of the altered tissues. OBJECT AND METHODS: The histological specimens prepared from the operative materials of 196 patients with different forms of malignant and benign neoplasms were stained with Congo red and Thioflavin T and studied under the light and polarization microscope. RESULTS: The various forms of ß-stacked protein aggregates (ß-SPA) inclusions were detected in amyloids, keloid tissue, benign polyps, and several malignant tumors. CONCLUSION: The formation of non-functional protein aggregates proves the complex character of the impairment of protein metabolism resulting in local or systemic accumulation of secondary protein toxins results in ß-SPA formation as the self-sustaining complex of parametabolic processes. The ß-SPA formation is of considerable interest since their properties lead to the impairment of the normal physiological processes in adjacent tissues ensuring the chronic course of the pathology.
Subject(s)
Neoplasms/diagnosis , Neoplasms/metabolism , Protein Aggregates , Protein Aggregation, Pathological , Amyloid/metabolism , Biomarkers , Humans , Immunohistochemistry/methods , Neoplasm Grading , Neoplasms/surgeryABSTRACT
In experiments with normal and thymectomized rats it has been revealed that exposure of normal animals to 0.35 Gy of gamma-radiation induces changes in blood cells subsets, depression of NK functional activity, decrease in polymorphonuclear leukocyte basal chemiluminescence level, suppression of the delayed type of hypersensitivity reaction and stimulation of local IgE-synthesis in respiratory organs. At the same time irradiation of adult thymectomized animals did not resulted in significant fluctuations in cellular and humoral immunity indices as well as blood cells functional activity level. Some mechanisms of radiation-induced immune system disturbances mediated via thymus are discussed.
Subject(s)
Immune System/radiation effects , Radiation Injuries, Experimental/immunology , Thymus Gland/immunology , Animals , Antibody Formation/radiation effects , Hypersensitivity, Delayed/immunology , Hypersensitivity, Immediate/immunology , Immunity, Cellular/radiation effects , Immunoglobulin E/biosynthesis , Killer Cells, Natural/immunology , Killer Cells, Natural/radiation effects , Lung/immunology , Male , Radiation Dosage , Rats , Thymus Gland/radiation effects , Trachea/immunologyABSTRACT
The content of serum corticosterone so as immunological and nonspecific reactivity in rats exposed to single or fractionated gamma-radiation (0.35 Gy) were studied during 1 month. It has been revealed that corticosterone level, dynamics of absolute amount of leucocytes, lymphocytes, Rc-receptor-bearing cells in blood, relative content of FcR(+)-cells in bone marrow, spleen and peripheral lymph nodes of irradiated animals depend on the scheme of irradiation. Single irradiation didn't significantly influence natural and antibody-dependent killer cells activity and basal chemiluminescence level of polymorphonuclear cells, while the fractionated irradiation in equal dose induced considerable fluctuations of these parameters. The direction and intensity of the disturbances depend on the time after irradiation.