ABSTRACT
Cholesterol-lowering activity of probiotic strains of lactic acid bacteria genera Lactobacillus and Bifidobacterium in the in vivo experiments on the model of experimental hypercholesterolemia in mice was studied. It is established that the prophylactic scheme of introduction of probiotic cultures is more effective than therapeutic one for the manifestation of cholesteraze activity of probiotic cultures. The most effective were the cultures: L. acidophilus and B. bifidum, as well as the composition B. bifidum + B. longum. Cholesterol-lowering activity of the studied strains and their compositions in this experiment ranged between 40-78%. It is noted that cholesteraze activity of other studied strains was not lower and in some cases, higher than that of most of the drugs currently used in cholesterinozis.
Subject(s)
Bifidobacterium/enzymology , Cholesterol/blood , Hypercholesterolemia , Lactic Acid/metabolism , Lactobacillus/enzymology , Probiotics/metabolism , Animals , Cholesterol/administration & dosage , Female , Food, Formulated , Hypercholesterolemia/blood , Hypercholesterolemia/diet therapy , Hypercholesterolemia/prevention & control , Mice , Mice, Inbred BALB C , Probiotics/administration & dosageABSTRACT
The investigation of cytotoxicity and antibacterial activity of the novel thrombin inhibitors containing retro-D-sequences -D-Arg-D-Phe--modified by D-arginine amino group by the residues of lauric acid or chromone-contained substituent, in comparison with known cationic preservative Nalpha-lauroyl-L-arginine ethyl ester (LAE) have been carried out. It has been shown that compound Laur-D-Arg-D-Phe-OMe has a similar cytotoxicity with LAE, and Chrom-D-Arg-D-Phe-OMe has almost twice higher toxicity than it fatty moiety contained analogues. Antibacterial activity of Laur-D-Arg-D-Phe-OMe against Staphylococcus aureus and Bacillus subtilis is close in action to LAE. It is assumed that ability of thrombin inhibitors to suppress the growth of some microorganisms can be explained by their ability to suppress activity of trypsin-like serine proteinases, which participate in the infection process of Staphylococcus aureus and influence on Bacillus subtilis sporulation. These findings open new prospects for exploring efficient antimicrobial agents among synthetic low-molecular trypsin-like serine proteinase inhibitors.
Subject(s)
Anti-Bacterial Agents/pharmacology , Arginine/analogs & derivatives , Oligopeptides/pharmacology , Thrombin/antagonists & inhibitors , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/toxicity , Arginine/chemistry , Arginine/pharmacology , Arginine/toxicity , Bacillus/drug effects , Bacillus/enzymology , Cells, Cultured , Male , Mice , Microbial Sensitivity Tests , Molecular Structure , Oligopeptides/chemistry , Oligopeptides/toxicity , Staphylococcus aureus/drug effects , Staphylococcus aureus/enzymology , SwineABSTRACT
Experimental data have been presented which prove antibacterial efficiency of interferon preparations and inducers under infection processes evoked by Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae. It is shown that the system of phagocytizing cells and natural cells-killers plays the basic protective role in the organism under the persistence of these microbes. The basic regularities of activating effect of interferon preparations and its inducers on the functional activity of the above mentioned cells have been found. The methods of treatment with interferon drugs based on experimental data have been developed for the first time. They were used to cure patients with pyo-septic diseases.
