Influence of dietary replacement of fish meal with fish soluble meal on growth and TOR signaling pathway in juvenile black sea bream (Acanthopagrus schlegelii).

An 8-week feeding trial was conducted to evaluate the effect of replacement of fish meal (FM) with fish soluble meal (FSM) on growth performance, feed utilization and expression of genes involved in TOR signaling pathway for juvenile black sea bream (Acanthopagrus schlegelii). Six isonitrogenous (41%) and isolipidic diets were prepared to contain graded levels of FSM which replaced 0% (control diet), 10%, 20%, 30%, 40% and 60% protein from FM. Triplicate groups of 20 fish with initial weight 0.51 ± 0.01 g were fed with experimental diets twice daily to apparent satiation. The results showed significant differences in growth performance and feed utilization among all treatments, final body weight (FBW), percent weight gain (PWG), specific growth rate (SGR) and protein efficiency ratio (PER) significantly increased with dietary replacement levels of FM with FSM increasing from 0% to 40% (P < 0.05), PWG, SGR and PER were significantly reduced when replacement of FM with FSM further increased from 40% to 60%. Based on PWG against replacement levels of FM with FSM, A two-slope broken-line model analysis indicated that the optimal replacement of FM with FSM is to be 42.59%. Moreover, the lowest feed conversion ratio (FCR) was observed in fish fed the 40% FSM replacement diet. Muscle amino acid profile in muscle revealed that total essential amino acids, arginine and threonine were significantly influenced by replacement levels of FSM, while there was no significant difference in NEAA among all treatments. The hematological indices were not affected by the replacement levels of FM with FSM. The relative expression levels of irs-1, pi3k, akt, igf-1, s6k1 and tor were up-regulated when replacement levels of FM with FSM increased from 0% to 40%, and higher values were observed in fish fed with 40% FSM replacement diet compared to those fed the other diets. However, relative expression of 4e-bp2 was down-regulated when replacement levels of FM with FSM increased from 0% to 40% (P < 0.05). In summary, the results of present study indicated that FSM could be a viable alternative protein source for black sea bream, dietary FSM supplementation could improve growth and up-regulate the relative expression of irs-1, pi3k, akt, igf-1, s6k1 genes related to TOR signaling pathway in liver of juvenile black sea bream.

The effects of dietary yeast hydrolysate on growth, hematology, antioxidant enzyme activities and non-specific immunity of juvenile Nile tilapia, Oreochromis niloticus.

The present study was aimed to compare and evaluate the impacts of supplemented diets with different yeast hydrolysate (YH) levels on growth performance, body composition, hematological characteristics, antioxidant enzyme activities, and non-specific immunity (intestinal cytokines) of juvenile Nile tilapia (Oreochromis niloticus). Three isonitrogenous (protein, 33%) and isolipidic (lipid, 6%) experimental diets supplemented graded levels of YH (0% for control; 1% and 3% as tested diets) were fed to juvenile Nile tilapia. A total of 240 fish with initial body weight averaging 3.5 ± 0.02 g were randomly divided into three groups with four replicates per group and 20 fish for each replicate. For apparent satiation, the fish were fed twice daily during eight weeks. The results showed no significant difference in survival among all treatments. The fish fed the diet containing 1% yeast hydrolysate had significantly elevated weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) compared to the control group and lower feed conversion ratio (FCR). The fish fed 1% and 3% YH showed higher glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) activity and a significantly lower malondialdehyde (MDA) level in the liver than the control group, indicating enhancement of the anti-oxidant status. Serum lysozyme activity was significantly increased in the diet having 1% and 3% yeast hydrolysate supplementation groups, suggesting an improvement influence on the non-specific immune response. The expression of IL-1ß, IL-10, TNF-α, TGF-ß2, ALP and TLR2 was significantly elevated in fish fed the diet containing 1% YH. In conclusion, dietary supplementation with 1% yeast hydrolysate improves growth performance, and feed utilization enhances the antioxidant status and exerts an adequate stimulus on the non-specific immunity (intestinal cytokines) of Nile tilapia.