Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Type of study
Language
Publication year range
1.
Mol Cell Biol Res Commun ; 3(5): 306-11, 2000 May.
Article in English | MEDLINE | ID: mdl-10964755

ABSTRACT

Paracentrotus lividus embryos, at post-blastular stage, when subjected to a rise in temperature from physiologic (20 degrees C) to 31 degrees C, synthesize a large group of heat shock proteins (hsps), and show a severe inhibition of bulk protein synthesis. We show, by mono- and two-dimensional electrophoresis, that also EGTA (ethylene glycol-bis[beta-aminoethyl ether] tetraacetic acid) treatment induces in sea urchin embryos both marked inhibition of bulk protein synthesis and the synthesis of the entire set of hsps. Furthermore, EGTA-treated sea urchin embryos are able to survive at a temperature otherwise lethal (35 degrees C) becoming thermotolerant. Because incubation with a different calcium-chelator, EDTA (ethylenediaminetetraacetic acid), or in calcium-free medium did not induce hsps synthesis we conclude that the stress response caused by EGTA is not related to its calcium chelator function.


Subject(s)
Chelating Agents/pharmacology , Egtazic Acid/pharmacology , Embryo, Nonmammalian/drug effects , Heat-Shock Proteins/biosynthesis , Sea Urchins/embryology , Animals , Edetic Acid/metabolism , Electrophoresis, Gel, Two-Dimensional , Embryo, Nonmammalian/metabolism , Heat-Shock Proteins/metabolism , Sea Urchins/physiology
2.
Biochem Biophys Res Commun ; 240(2): 359-66, 1997 Nov 17.
Article in English | MEDLINE | ID: mdl-9388482

ABSTRACT

It is demonstrated by DNA electrophoresis analysis, morphological observations and TdT in situ reaction, that Paracentrotus embryos if treated with TPA plus heat undergo an apoptotic reaction. Indication is also obtained that non treated embryos undergo spontaneous apoptosis at the early pluteus stage, especially in the districts of arms and intestine. The possible meaning of this latter observation is discussed.


Subject(s)
Apoptosis , Embryo, Nonmammalian/physiology , Gastrula/physiology , Sea Urchins/embryology , Animals , DNA Fragmentation , Embryo, Nonmammalian/cytology , Embryonic Induction , Gastrula/cytology , Gastrula/drug effects , Tetradecanoylphorbol Acetate/pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL
...