ABSTRACT
The activities of lactate dehydrogenase (LDH), glucose 6-phosphate dehydrogenase (G6PD) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) in two zones of the epithelium of the lens of 6- and 27-month-old rats were measured using quantitative cytochemical methods. In both age groups, the epithelium in the equatorial zone showed the highest enzymatic activity. LDH activity was similar in young and old rats in both areas. G6PD and GAPD activities in the central area were similar in both age groups, but their activity was markedly lower in the equatorial zone in old rats compared to young ones. This decrease in G6PD and GAPD activities may play a role in cataract formation.
Subject(s)
Aging/metabolism , Lens, Crystalline/enzymology , Animals , Epithelium/metabolism , Glucosephosphate Dehydrogenase/metabolism , Glycerolphosphate Dehydrogenase/metabolism , L-Lactate Dehydrogenase/metabolism , Rats , Rats, Inbred StrainsABSTRACT
An experimental study was performed to assess the possible tumour-promoting action of urothelial trauma caused by repeated intravesical diathermy, catheterisation, and the instillation of water, glycine and saline in the rat bladder which had previously been exposed to an initiating dose of the carcinogen N-methyl-N-nitrosourea. An increase in tumours and inflammatory changes was observed, especially in the group treated with water, and the significance of the findings is discussed.
Subject(s)
Cystoscopy/adverse effects , Urinary Bladder Neoplasms/pathology , Animals , Catheterization/adverse effects , Diathermy/adverse effects , Female , Glycine/adverse effects , Inflammation/etiology , Methylnitrosourea , Organ Size , Rats , Rats, Inbred Strains , Sodium Chloride/adverse effects , Urinary Bladder Neoplasms/chemically induced , Water/pharmacologyABSTRACT
The staining method uses alizarin red S and has been adapted for a 4-5 day cycle on a standard tissue processor. It gives consistently well stained bones and clear soft tissues in both rats and mice.