ABSTRACT
A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000-1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.
Subject(s)
DNA, Mitochondrial/genetics , Evolution, Molecular , Genetic Variation , Sheep/genetics , Animals , Archaeology , Breeding , DNA, Ancient/analysis , Haplotypes , Phylogeny , Sequence Analysis, DNA/veterinary , SiberiaABSTRACT
From genetic point of view, differences between ancient and modern horses can be reconstructed by using the phylogeographic analysis of mitochondrial genomes and by studying phenotypically important nuclear loci. The variety of modern horse coat colors resulted from artificial selection indicates a high degree of domestication. We have conducted the phylogenetic analysis of mitochondrial DNA extracted from bone samples of six ancient horses from Tsaramburial in the Republic of Buryatia, and established that they belong to a haplogroup E by Achilli's classification. This haplogroup is found among modern horses of the Maremmano breed from Italy. Gray coat color different from wild type have been detected in two ancient horses, which demonstrates a sufficiently high domestication level of Buryat horses during the period I century BC to I century AD. The analysis of the mitochondrial genome hypervariable region fragments revealed that ancient Buryat horses belong to a haplotype X3 by Cieslak's classification, which is ancestral to the haplogroup X3 of modern horses in Mongolia, Tuva, and Buryatia.
