[Changes in rat liver lysosomes following stimulation of recovery processes in the damaged organ by triton WR-1339]. / Osobennosti izmenenii lizosom pecheni krys pri stimuliatsii vosstanovitel'nykh protsessov tritonom WR-1339 v povrezhdennom organe.

The effect of a single injection of lysosomotropic agent--Triton WR-1339 on the properties of the rat liver lysosomes and the course of pathological process during chronic toxic hepatitis were studied. Triton WR-1339 administration was followed by a more rapid restoration of the liver structure and function. A possible mechanism of the favourable effect of Triton WR-1339 is discussed.

[Effect of triton WR 1339 on the ultrastructure of liver parenchymal and stromal cells in rats with chronic toxic hepatitis]. / Vliianie tritona WR 1339 na ul'trastrukturu kletok parenkhimy i stromy pecheni krys pri khronicheskom toksicheskom gepatite.

A preliminary injection of triton WR 1339 protectively influenced the chronic toxic CCl4-hepatitis. The ultrastructural peculiarities were close to the normal ones in hepatocytes, in addition to the increased triglyceride rejection from cells by means of autophagy. The revealed stimulation signs of pinocytosis in hepatocytes and the Kupfer cells are accompanied by the activation of the Golgi complex and, probably, by the increased protein catabolism in lysosomes. The signs of phagocytosis increase in the Kupfer cells were determined. It is supposed that the marked ultrastructural peculiarities of liver cells reflect a more effective restoration or a lesser injury under the influence of triton WR 1339.

[Effect of a lysosome triton WR 1339 load on distribution of C14-albumin in the livers of rats with chronic hepatitis]. / Vliianie nagruzki lizosom tritonom WR 1339 na raspredelenie al'bumina-C14 v pecheni krys s khronicheskom gepatitom

A possible mechanism of the protective effect of Triton WR1339 during chronic CC14-hepatitis was considered. Supposing that the improvement of the process was due to the intensification of the lysosome heterophage function the authors studied the intensity of the C14-albumin uptake by the liver and its subcellular distribution in the liver of rats in the administration of the detergent to the animals with chronic CCI4-hepatitis, Preliminary administration of the detergent failed to influence the intensity of C14-albumin uptake; subsequent administration of triton WR1339 to rats with toxic hepatitis decrease the protein uptake which reached the values in intact rats. In chronic hepatitis C14-albumin was concentrated in the lysosome fraction. Administration of trition WR1339 to CCI4-treated animals was not accompanied by any coincidence in the peaks of labeled protein and lysosome enzymes. The selective participation of lysosomes of the Kupffer cells providing a more rapid restoration of the liver in chronic hepatitis is discussed.

[Intracellular distribution of hyaluronidase in rats with acute and chronic hepatitis and reparative liver regeneration]. / Vnutrikletochnoe raspredelenie gialuronidazy pri ostrom i khronicheskom gepatite i reparativnoi regeneratsii pecheni krys

Subcellular distribution of hyaluronidase of rat liver was studied during spontaneous restoration during the 1st, 2nd, 3rd days, and 1, 2, 3, 4 weeks of acute and chronic CCl4-hepatitis. Redistribution of relative specific activity of hyaluronidase was seen in the fractions of heavy and light mitochondria, microsomes and supernatant fraction, after an acute hepatic injury. Four weeks after the injury subcellular distribution was not yet normal. In chronic hepatitis the relative specific hyaluronidase activity was almost identical in the five subcellular fractions. In the course of restoration the relative specific activity was increased in the fractions of heavy and light mitochondria and microsomes, but decreased in the nuclear and supernatant fractions.

[Changes in the liver lysosomes of the rat in chronic toxic hepatitis]. / Izmeneniia lizosom pecheni krys pri khronicheskom toksicheskom gepatite

A study was made of permeability of the lysosome membranes and subcellular distribution of acid hydrolases in chronic hepatitis caused by CCl4 inhalation and during the restoration of the liver after injury. No normalization of the indices under study occurred during the period of up to 14 days after the last CCl4 inhalation: changes in the stability of the lysosome membran persisted and redistribution of acid hydrolases was noted. This redistribution was associated with both the processes of injury and restoration of the liver.

[Subcellular distribution of acid hydrolases in rat liver during toxic hepatitis]. / Subkletochnoe raspredelenie kislykh gidrolaz pecheni krys pri toksicheskom gepatite

Changes occurring in the lysosome population were assessed by the results of studies of intracellular distribution of the marker lysosome enzymes--acid phosphatase and acid RNAase. An acute (pure CCl4-0.15 ml per 100 g of weight into the stomach) and chronic (inhalation poisoning after Rabinovici and Wiener) toxic hepatitis was accompanied by an increase in the specific activity of the enzymes in the fraction of heavy mitochondria, this pointing to the change in the sedimentation properties of the lysosomes. An increase in "nonprecipitable" activity of the acid RNA-ase in chronic toxic hepatitis served as the sign of injury of the lysosome membranes.

[The effect of loading liver lysosomes with triton WR 1339 on the development of chronic toxic hepatitis]. / Vliianie nagruzki lizosom pecheni tritonom WR 1339 na razvitie khronicheskogo toksicheskogo gepatita

Preliminary administration of triton WR 1339 produced a favourable effect on the course of chronic toxic hepatitis. This was expressed in a reduction of necrotic zones, a delay in development of connective tissue and in improvement of the functional capacity of the liver. Lysosomes of the liver of animals subjected to the action of CCl-4 under conditions of preliminary administration of a detergent were more stable to the injurious actions in vitro.