A review on efforts for improvement in medicinally important chemical constituents inAconitum through biotechnological interventions.

The genus Aconitum belongs to the family Ranunculaceae, is endowed with more than 350 species on the earth. Medicinally important aconitine type of diterpenoid alkaloids are the characteristic compounds in most of the Aconitum species. The present review endeavored the major research carried out in the field of genetic resource characterization, pharmacological properties, phytochemistry, major factors influencing quantity, biosynthetic pathways and processing methods for recovery of active ingredients, variety improvement, propagation methods, and important metabolite production through cell/organ culture of various Aconitum species. More than 450 derivatives of aconitine-type C19 and C20-diterpenoid alkaloids along with a few other non-alkaloidal compounds, such as phenylpropanoids, flavonoids, terpenoids, and fatty acids, have been identified in the genus. A few Aconitum species and their common diterpenoid alkaloid compounds are also well characterized for analgesic, inflammatory and cytotoxic properties. However, the different isolated compound needs to be validated for supporting other traditional therapeutical uses of the plant species. Aconitine alkaloids shared common biosynthesis pathway, but their diversification mechanism remains unexplored in the genus. Furthermore, the process needs to be developed on secondary metabolite recovery, mass-scale propagation methods, and agro-technologies for maintaining the quality of products. Many species are losing their existence in nature due to over-exploitation or anthropogenic factors; thus, temporal monitoring of the population status in its habitat, and suitable management programs for ascertaining conservation needs to be developed.

An overview of the phytosynthesis of various metal nanoparticles.

Nanotechnology is an emerging branch of science wherein various valuable molecules with altered properties can be synthesized and utilized for numerous technological applications. Nowadays, nanotechnology is the preferred tool for the agriculture, food, and medicine industries. However, consistent accumulation of toxic by-products during the synthesis of nanoparticles from the established physical and chemical methods imposes an unprecedented danger to the environment and human well-being. The biological route for the synthesis of nanoparticles offers a potential option over the conventional chemical synthesis process due to the involvement of non-toxic and environmentally friendly materials, such as plants, fungi, bacteria, etc. Phytosynthesis, a type of biological synthesis, utilizes various combinations of secondary metabolites from different plant parts (whole plant, leaves, fruit peel, root, bark, seeds, and stem) for non-toxic and environmentally friendly nanoparticles fabrication. Non-toxic and environmentally friendly secondary metabolites derived from plants are the sources of reducing and capping agents during the biosynthesis of nanoparticles which proceeds in a controlled manner with desired characteristics. Phytosynthesis of nanoparticles is also a simple, economic, durable, and reproducible process. The present article is a comprehensive depiction of the synthesis of different metal nanoparticles from diverse plant species.