Subject(s)
Bone and Bones/diagnostic imaging , Diphosphonates , Organotechnetium Compounds , Blood Proteins/metabolism , Chemical Phenomena , Chemistry , Diphosphonates/metabolism , Diphosphonates/pharmacokinetics , Humans , Organotechnetium Compounds/metabolism , Organotechnetium Compounds/pharmacokinetics , Protein Binding , Radionuclide ImagingABSTRACT
The binding of 99mTc(Sn)-MDP to human serum albumin and other blood proteins was investigated by gel chromatography and ultrafiltration. During gel chromatography dissociation of the 99mTc(Sn)-MDP-protein complex occurs: thus, it is not a suitable technique for the determination of protein binding. The values found with ultrafiltration have to be corrected for non-ultrafiltrable TcO2.nH2O. From the corrected values it can be concluded that binding of 99mTc(Sn)-MDP to blood proteins does not play a role in the biodistribution.
Subject(s)
Blood Proteins/metabolism , Serum Albumin/metabolism , Technetium Tc 99m Medronate/metabolism , Chromatography, Gel , Humans , UltrafiltrationABSTRACT
99mTc(Sn)-MDP complexes have been prepared by reduction of 99mTcO4- by Sn(II) in the presence of MDP. These complexes were separated on an anion exchange column. The necessity of the addition of the ligand and the reducing agent to the eluent to avoid decomposition during chromatography is demonstrated. For the main component that is found at pH 5 the ionic charge was calculated according to the method of Wilson and Pinkerton [Anal. Chem. 57,246 (1985)] and the method of Russell and Bishoff [Int. J. Appl. Radiat. Isot. 35,859 (1985)]. With the first method a charge of -4.2 +/- 0.3 was obtained, with the second a charge of -5.1 +/- 0.6. An accurate method to determine the void volume of the ion exchange column is described.
Subject(s)
Technetium Tc 99m Medronate , Chromatography, Ion Exchange , Hydrogen-Ion Concentration , Indicators and Reagents , Molecular Conformation , Technetium Tc 99m Medronate/chemical synthesisABSTRACT
The effect of addition of Sn(IV), Mg(II) and Ca(II) on the mean size of the 99mTc-containing constituents of a 99mTc(Sn)MDP reaction mixture was determined by a gel chromatographic batch procedure that does not disturb the equilibria in the mixture.
