ABSTRACT
The aim of this investigation is to study the number of circulating tumor cells (CTCs) in the blood of cancer patients after systemic photodynamic therapy (PDT) at different times and to assess apoptosis of these cells. The study group consisted of 19 patients with malignant tumors of epithelial origin at various stages (II-IV). CTC identification was performed with flow cytometry by immunophenotype Ep-CAM (CD326)+ CD45-. CTC apoptosis was identified by criteria of plasma membrane integrity and phosphatidylserine translocation on the outer surface of the membrane. Negative correlation between the CTC frequency and apoptotic death rate of these cells was found in patients before the treatment (R = -0.51, p = 0.03). CTC frequency gradually reduced during the first three days after PDT, and then it was maintained at the same level until the end of the follow-up (7 days). At the individual level, the effect of PDT depended on the frequency of CTCs before the treatment: the decrease in these cell frequency occurred significantly more often in the patients with an initially high frequency of CTCs than in other patients (p = 0.05). With the decrease in the CTC frequency, apoptotic death increased within 6 hours after the treatment and remained at the same level until the end of the follow-up period. The results demonstrate the efficacy of systemic PDT for elimination of tumor cells circulating in the peripheral blood of cancer patients with different localization of primary tumor and stage of disease.
Subject(s)
Breast Neoplasms/therapy , Lung Neoplasms/therapy , Neoplastic Cells, Circulating/radiation effects , Photochemotherapy , Adult , Aged , Apoptosis/radiation effects , Breast Neoplasms/pathology , Cell Count , Female , Flow Cytometry , Humans , Lung Neoplasms/pathology , Male , Middle AgedSubject(s)
Arteriosclerosis/radiotherapy , Cardiovascular Diseases/radiotherapy , Low-Level Light Therapy , Angina Pectoris/diagnosis , Angina Pectoris/radiotherapy , Animals , Arteriosclerosis/diagnostic imaging , Arteriosclerosis Obliterans/diagnosis , Arteriosclerosis Obliterans/diagnostic imaging , Arteriosclerosis Obliterans/radiotherapy , Cardiovascular Diseases/diagnosis , Cardiovascular Diseases/diagnostic imaging , Coronary Artery Disease/diagnosis , Coronary Artery Disease/radiotherapy , Diabetic Angiopathies/diagnosis , Diabetic Angiopathies/radiotherapy , Disease Models, Animal , Humans , Infrared Rays , Intracranial Arteriosclerosis/diagnosis , Intracranial Arteriosclerosis/radiotherapy , Leg/blood supply , Male , Photochemotherapy , Rats , Time Factors , Tomography, Emission-Computed , Treatment Outcome , Ultrasonography, DopplerABSTRACT
We present predictions on the total cross sections and on the rho parameter for present and future pp and (-)pp colliders, and on total cross sections for gammap-->hadrons at cosmic-ray energies and for gammagamma-->hadrons up to sqrt[s]=1 TeV. These predictions are based on an extensive study of possible analytic parametrizations invoking the largest hadronic dataset available at t=0. The uncertainties on total cross sections reach 1.9% at the Relativistic Heavy Ion Collider, 3.1% at the Tevatron, and 4.8% at the Large Hadron Collider, whereas those on the rho parameter are, respectively, 5.4%, 5.2%, and 5.4%.
ABSTRACT
30 patients with chronic ischemic heart disease allocated to controlled treatment and close monitoring, and 30 patients with ischemic heart disease placed under routine dispensary observation, living in rural areas, were examined. Effectiveness of dispensary follow-up of the IHD patients was found to be dependent upon three factors: use of differentiated approach to prescription of drugs, continuity of work in organization of treatment and feasibility of giving therapy under carefully health monitored conditions.
