Seroprevalence of antibodies to Chlamydia abortus and risk factors in cattle from Villavicencio, Colombia.

Chlamydia abortus is a Gram-negative obligate intracellular bacterium, responsible for abortions and reproductive problems. The disease has a high zoonotic potential and causes great economic losses in ruminant farmers. A cross-sectional study was carried out with 514 cattle from 24 farms of Villavicencio, Colombia. The blood samples were collected from each individual animal and analyzed by Indirect Elisa for immunoglobulin G (IgG) in blood serum (Idexx Chlamydiosis total Ab test). A serum was considered positive when the optical density (OD) of the sample was ≥30% of that of the positive control serum. Data on potential risk factors associated with the disease were collected through a questionnaire in each farm and analyzed. The individual and herd prevalence was estimated. A risk factors analysis was performed through univariate and multivariable using the software SPSS version 20. The animal level seroprevalence was found to be 47.1% and the herd 91.6%. The prevalence in cattle aged 0-1, 1-3 and >4 years was 23.8%; 31.4% and 51.4% respectively. The risk factors associated with the prevalence of disease were female sex (OR = 2.102 CI: 1.066-4.144), age older than 4 years (OR = 2.707 CI: 1.667-4.394), presence of canines on the farm (OR = 2.556 CI: 1.560-4.189) and retention of placenta (OR = 2.678 CI: 1.670-4.295). A high prevalence was identified, suggesting natural infection where the pathogen could be transmitted to humans at the animal-human interface.

Current status of prevalence and risk factors associated with liver fluke Fasciola hepatica in cattle raised in different altitudinal regions of Colombia.

Fasciola hepatica is a trematode parasite with a worldwide distribution that affects mammals, including humans, and is considered a major food safety and public health problem. A research was conducted in three hundred eighty-five livestock farms located in different altitudinal regions of Colombia between February 2017 and March 2018 with the aim to determine the coprological prevalence, seroprevalence (SP) and risk factors associated with liver fluke F. hepatica. A total of 5620 fecal sample were collected from cattle and processed using sedimentation technique. Additionally, 597 blood samples were collected and processed by an ELISA test. The coprological prevalence of F. hepatica in animals and herds was 6.4% and 38.4%, respectively. Regarding the prevalence by municipalities, there was statistical association (P < .05), indicating that the prevalence was higher in those municipalities above 2000 masl (meters above sea level). Regarding risk factors, cattle raised in municipalities at 2000 to 3000 masl showed about 4 (OR = 3.96 [CI95% 3.1-5]; P < .05) times higher probability for infection than those raised at lower altitude. Cattle from 2 to 3 year of age, showed almost 4 times (OR = 3.7[CI95% 2.5-5.4]; P < .05), increased infection risk. Females and dairy breeds showed almost 2 (OR = 1.9 [CI95% 1.3-2.6]; P < .05) and 10 times (OR = 10 [CI95% 7.8-12.8]; P < .05), respectively, increased infection risk. The presence of antibodies anti- F. hepatica (SP = 37.8%; [CI95% 35.8-39.7]; P < .05) in cattle of the region under study is confirmed, which suggests an endemic behavior of this parasitosis. The present study provides current information on the prevalence of F. hepatica in different altitudinal regions of Colombia, and these findings could be useful for designing new holistic control measures for the liver fluke F. hepatica infection.

Prevalence and molecular epidemiology of bovine leukemia virus in Colombian cattle.

Bovine leukemia virus (BLV) is one of the five agents considered most significant for cattle. It is important to determine the prevalence and molecular epidemiology of BLV throughout the country in order to gain a more thorough understanding of the current situation of BLV and to reveal the possibility of masked genotypes that the primers used by OIE are unable to identify. Blood samples were collected at random from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. A total of 62% of the cows present at 92% of the farms were BLV-positive for gag fragment. Genotype 1 was exclusively detected by env gene segment when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. This report is one of the first molecular and epidemiological studies of BLV conducted in Colombia, which contributes to the global epidemiology of the virus; it also highlights the substantial impact of BLV on the country's livestock and thus is a useful resource for farmers and government entities.

Hidrocoloides como estabilizantes en bebidas de maíz dulce (Zea mays var. saccharata) y gel de aloe vera ( Aloe barbadensis Miller) / Hydrocolloids as stabilizers in beverages from sweet corn (Zea mays var. Saccharata) and aloe vera gel (Aloe barbadensis Miller)

RESUMEN La sedimentación de partículas es un fenómeno común en dispersiones alimentarias, que está asociado con una calidad deficiente y se puede controlar usando diferentes aditivos, dentro de los cuales, se destacan el uso de hidrocoloides. Se evaluó el efecto de la concentración de goma xantano (0,025-0,075%), carboximetilcelulosa sódica (0,025-0,075%) y gel de aloe vera (0,5-1,5%), en las propiedades fisicoquímicas, sensoriales y estabilidad a la sedimentación, en bebidas elaboradas a partir de maíz dulce. No se observó variación significativa en el contenido de sólidos solubles totales, pH, densidad, acidez titulable, potencial ζ, tamaño de partículas, velocidad de sedimentación y parámetros de color con las concentraciones empleadas de hidrocoloides y de aloe vera. El potencial ζ indicó estabilidad ligera en las bebidas (>-30mV) y la floculación de la emulsión, lo que se evidencia en la distribución del tamaño de partícula (1-600μm). La viscosidad y la estabilidad a la sedimentación aumentaron con concentraciones >0,05% de GX y CMC, reflejándose en una mayor valoración sensorial de la apariencia. El aumento en la concentración de aloe vera no mostró efecto en la estabilidad de las bebidas.

ABSTRACT Particle sedimentation is a common phenomenon in food dispersions, which is associated with poor quality and can be controlled using different additives within which the use of hydrocolloids stands out. The effect of the concentration of xanthan gum (0.025-0.075%), sodium carboxymethylcellulose (0.025-0.075%) and aloe vera gel (0.5-1.5%), on physicochemical, sensory properties and sedimentation in beverages made from sweet corn, were evaluated. No significant variation was observed in the content of total soluble solids, pH, density, titratable acidity, ζ potential, particle size, sedimentation index and color parameters with the concentrations of hydrocolloids and aloe vera. The potential ζ indicated slight stability in beverages (> -30mV) and emulsion flocculation, which is evidenced in the particle size distribution (1-600μm). The viscosity and sedimentation stability increased with concentrations >0.05% of GX and CMC, reflected in a higher sensory assessment of appearance. The increase in aloe vera concentration showed no effect on the stability of the beverages.

Seroprevalence and risk factor associated with respiratory viral pathogens in dual-purpose cattle of Aguachica, Rio de Oro, and La Gloria municipalities in Cesar department, Colombia.

AIM: The research was conducted to determine the seroprevalence and risk factor associated with respiratory viral pathogens in dual-purpose cattle of Aguachica, Rio de Oro and La Gloria municipalities in Cesar department, Colombia. MATERIALS AND METHODS: The seroprevalence study was done from the random sampling (n=1000) of blood collected from 29 dual-purpose herds, located in three municipalities (Aguachica, Rio de Oro, and La Gloria) of Cesar department. The presence of antibodies against bovine herpesvirus type 1 (BHV-1), bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), and bovine parainfluenza-3 virus (BPI-3V) in the samples was detected by indirect enzyme-linked immunosorbent assay. Epidemiological data were obtained using a questionnaire administered to the owner or manager of each herd. RESULTS: The overall highest seroprevalence was observed for BHV-1 (94.7%), followed by BRSV (98.6%), BVDV (35.2%), and BPI-3V (47.1%). Regarding the seroprevalence by municipalities, there was a statistical association (p<0.05) for BVDV; however, for BRSV, BHV-1, and BPI-3V, no statistical association was found (p>0.05) between seropositive values and the municipalities, indicating that animal was seropositive in similar proportions in the three municipalities. Female sex and older animals (>24 months) were a significant risk factor for BHV-1 and BPI-3V infection. Regarding the clinical signs, there was a statistical association (p<0.05) between the seropositive values of BVDV and most of clinical signs observed, except for abortion. CONCLUSION: This research confirms the high seroprevalence of the respiratory viral pathogens in nonvaccinated cattle within the study areas. Therefore, appropriate sanitary management practices and routine vaccination programs should be adopted to reduce the seroprevalence of these infectious agents.

Influencia de un recubrimiento comestible adicionado con calcio sobre la calidad de la mora de Castilla / Influence of edible coating added with calcium on andean blackberry quality

RESUMEN La creciente necesidad de los consumidores por alimentos saludables ha suscitado, en la industria, la búsqueda de tecnologías efectivas, que ofrezcan alimentos seguros y aceptables, sensorialmente, por el consumidor moderno. Los recubrimientos comestibles adicionados con componentes activos aplicados en estructuras vegetales, además de proteger la estructura y conferir mayor conservación, pueden aportar valor nutricional. El objetivo de la investigación fue evaluar la influencia de un recubrimiento comestible a base de alginato de sodio y calcio sobre los atributos de calidad de la mora de Castilla. Se aplicó la metodología de superficie de respuesta, con un diseño central compuesto cara centrado (21 experimentos), considerando las variables independientes: alginato de sodio (2,0-3,0%), cera de abeja (0,5-1,0%), sucroéster (0,15-0,25%), alginato/glicerol (2,0-3,0). La mejor formulación fue: alginato de sodio (2,85%), cera de abeja (1,00%), alginato de sodio/glicerol (2,0) y sucroéste (0,18%), siendo los atributos de calidad de la mora con el recubrimiento comestible: humedad (83,7±1,7%), actividad de agua (0,964±0,010), pH (2,7±0,0), acidez (2,6±0,0%), °Brix (8,1±0,2%), firmeza (12,3±0,7 N), fenoles totales (105,3±4,5mg AG/100g), actividad antioxidante (ABTS: 962,7±15,1mg Trolox/100g y DPPH: 319,3±9,4mg Trolox/100g), luminosidad (22,5±0,7); cromaticidad a* (8,47±0,88); cromaticidad b* (2,12±0,37) y calcio = 144,6±2,7 mg /200g. Los recubrimientos comestibles adicionados con calcio aplicados en la mora de Castilla representan una alternativa efectiva contra los daños mecánicos del fruto y para conservar su calidad, lo cual, podrá mejorar la vida útil durante el almacenamiento.

ABSTRACT The growing need of consumers for healthy food has led to the search for effective technologies in food industry that offer safe and sensorially acceptable options to the modern consumer. Edible coatings added with active components applied to vegetable structures, besides protecting the structure and conferring greater conservation, can provide nutritional value. The objective of this investigation was to evaluate the influence of an edible coating based on sodium alginate and calcium on the attributes of quality of Andean blackberry of Castilla. The response surface methodology was applied with a composite central design (21 experiments), considering the independent variables: sodium alginate (2.0-3.0%), beeswax (0.5-1.0%), sucroester (0.15-0.25%), sodium alginate/glycerol (2.0-3.0). The best formulation was: sodium alginate (2.85%), beeswax (1.00%), sodium alginate/glycerol (2.0%) and sucroester (0.162%), being the quality attributes of the of the blackberry with the edible coating: moisture (83.7±1.7%), water activity (0.964±0.010), pH (2.7±0.0), acidity (2.6±0.0%), °Brix (8.1±0.2%), firmness (12.3±0.7 N); total phenols (105.3±4.5 mg GA/100 g), antioxidant activity (ABTS: 962.7±15.1 mg Trolox/100 g) and DPPH: 319.3±9.4 mg Trolox/100 g), lightness (22,5±0,7); chromaticity a* (8,47±0,88); chromaticity b* (2,12±0,37) and calcium=144,6±2,7 mg/200g. Edible coatings added with calcium applied to Andean blackberry of Castilla represent an effective alternative against mechanical damages of the fruit and to conserve its quality, which will improve shelf life.

High prevalence of persistently infected animals from bovine viral diarrhea in Colombian cattle.

BACKGROUND: Bovine Viral Diarrhea Virus (BVDV) is associated with gastrointestinal, respiratory and reproductive diseases of livestock across the world that causes continuous economic losses in the cattle industry. This virus can establish a persistent infection (PI) in calves after the fetal infection, making BVDV positive catle carriers and primary reservoirs which will constantly transmit the virus to healthy and new-born animals. For this reason, the detection of the PI animals in herds is the first line of prevention of the viral infection. RESULTS: In this study, PI animals were detected in five different regions of Colombia through RT-PCR techniques and confirmed by sequencing. BVDV genotypes were determined using one fragment of the 5'UTR. It was found a 7% BVDV prevalence in animals and 22% in farms; and genotype 1 was identified as a single genotype for all of the samples. All samples were BVDV 1a. CONCLUSION: This is the first report in Colombia with higher prevalence rates compared with other places in the world, turned out to be of great importance for the ranchers, the vaccine producers and animal health control parties.

EFECTO DEL SECADO Y PRESECADO MECÁNICO PREVIO AL ALMACENAMIENTO EN LA CALIDAD DEL GRANO DE CAFÉ (Coffea arabica L.) / EFFECT OF DRYING AND MECHANICAL PRE-DRYING PREVIOUS TO STORAGE IN THE QUALITY OF COFFEE GRAIN (Coffea arabica L.)

RESUMEN El objetivo de este estudio fue evaluar el efecto de las condiciones de secado y presecado en muestras de café pergamino, almacenadas durante 6 meses, en condiciones controladas de humedad relativa, 70% y temperatura, 25°C, determinando la Conductividad Eléctrica (CE), Lixiviación de Potasio (LK) y Acidez Grasa (AG), de granos secos de café. El proceso de secado consistió en procesos combinados a temperaturas 50 y 60°C, dos periodos de 4 y 8 horas en el presecado mecánico y, posteriormente, llevado a humedad comercial en patio solar y dos tratamientos testigos, conformados por el secado mecánico completo y el secado solar completo. Los resultados mostraron los siguientes valores: 6,35 - 16,34µSm cm-1/ g MS, para CE; 2,0 - 4,45ppm/gMS, para LK y 0,76 - 1,57mL KOH/ 100g MS, para AG, donde los análisis estadísticos mostraron que no hubo efecto del tipo de secado sobre las variables estudiadas; sin embargo, los mayores promedios acumulados en el tiempo de almacenamiento fueron observados para el secado mecánico completo. Los valores encontrados no indicaron daños de la estructura celular atribuidos según, el marco referencial, a secados con alta temperatura o prolongado almacenamiento. Las pruebas de Conductividad Eléctrica (CE) y Acidez Grasa (AG) mostraron sensibilidad al tiempo de almacenamiento, mientras que la prueba de Lixiviación de Potasio (LK), no se constituyó en un indicador del deterioro del grano, atribuible al estrés termomecánico o al tiempo de almacenamiento.

ABSTRACT The aim of this study was to evaluate the effect of drying and pre-drying conditions of samples of parchment coffee stored during 6 months under controlled conditions of relative humidity of 70% and temperature of 25°C quantifying the Electrical Conductivity (EC), Potassium Leaching (KL) and Fatty Acidity (FA) of the grain. The coffee drying consisted on combined processes using air temperatures of 50°C and 60°C and two periods of 4 and 8 hours in the mechanical pre-drying and subsequently taken to commercial moisture content under patio drying, and two control treatments consisting of complete mechanical drying and complete solar drying. The results of the tests showed the following values: 6.35 - 16.34 µSm cm-1 / g DM (EC), 2.0 - 4.45ppm / g DM (KL) and 0.76 - 1.57mL KOH / 100g MS (FA), where the statistical analysis shows that there is no effect of the drying type on the studied variables. However, the highest accumulated averages in the storage time were observed for the complete mechanical drying. The values did not show deterioration of the cellular structure attributed to the frame of reference of drying with high temperature or prolonged storage. The tests of Electrical Conductivity (EC) and Fatty Acidity (FA) shows sensitivity to the storage time while the Potassium Leaching (KL) test did not constitute an indicator of grain deterioration attributable to thermomechanical stress or storage time.

PREVALENCIA DE Babesia caballi, Theileria equi Y TRIPANOSOMIASIS Y ANÁLISIS DE FACTORES DE RIESGO EN EQUINOS DE ANTIOQUIA, COLOMBIA / PREVALENCE OF Babesia caballi, Theileria equi AND TRYPANOSOMIASIS AND ANALYSIS OF RISK FACTORS IN HORSES OF ANTIOQUIA, COLOMBIA

RESUMEN Esta investigación, se llevó a cabo, debido a la poca información nacional existente en hemoparásitos equinos y su objetivo fue determinar la prevalencia de babesiosis y tripanosomiasis, en el Valle de Aburrá y Rionegro, municipios de Antioquia (Colombia), así como algunos factores de riesgo asociados a la presentación de seropositividad, a estas enfermedades. En 223 predios, con una población de 1.008 equinos, se tomó muestra de sangre venosa, para realizar el diagnóstico serológico y molecular. La información epidemiológica fue obtenida, a través de la aplicación de una encuesta dirigida a las personas a cargo de los animales. Para el análisis estadístico, se aplicó Chi cuadrado de independencia y la Prueba Exacta de Fisher, cuando fue necesario. Para las asociaciones bivariadas, se calcularon estimaciones del riesgo (OR) por variable explicativa, con sus respectivos intervalos de confianza, del 95% (I.C. 95%). Se encontró una prevalencia del 11,9%, para babesiosis y de 1,9%, para tripanosomiasis; como factor de protección, se encontró el hecho de salir a una feria. Para la piroplasmosis, el lugar geográfico, el sexo, estar castrado, ser positivos a Anemia Infecciosa Equina (AIE), estrongílidos u oxiurus fueron factores de riesgo. Para tripanosomiasis, el factor de riesgo fue estar infestado con Dermacentor nitens o ser mular. Se deben reforzar métodos de vigilancia epidemiológica activa, sobre todo, en casos de movilización continua de equinos o desarrollo de eventos, que involucren presencia masiva de ejemplares.

ABSTRACT This research was carried out due to the little existing national information on equine hemoparasites; the objective was to determine babesiosis and trypanosomiasis prevalence in the Aburrá Valley and Rionegro Antioquia (Colombia) as well as some risk factors associated with the seropositivitie of these diseases. In 223 lots with a population of 1.008 equine venous blood sample was taken for serological diagnosis and molecular. Epidemiological information was obtained through the application of a survey addressed to persons in charge of the animals. For the statistical analysis, Chi square of independence or Fisher's Exact Test was applied when necessary. For the bivariate associations, risk estimates (OR) were calculated by explanatory variable, with their respective 95% confidence intervals (95% CI).It was found a prevalence of 11.9% for babesiosis and 1.9% for trypanosomiasis and as protection factor found the fact of going to trade fairs. Geographic location, sex and being neutered, be positive for Equine Infectious Anemia (AIE), strongyles or oxiurus was a risk factor for piroplasmosis. For Trypanosomiasis the risk factor was to be infested with ticks Dermacentor nitens or be mule. Methods of active epidemiological surveillance should be reinforced, especially in cases of continuous mobilization of equines or development of events that involve massive presence of horses.

Prevalencia de parásitos gastrointestinales en equinos y factores de riesgo asociados en varias zonas de Antioquia, Colombia / Prevalence of gastrointestinal parasites in equines and associated risk factors in several areas of Antioquia, Colombia / Prevalência de parasitos gastrointestinais em equinos e fatores de risco associados em várias zonas de Antioquia, Colômbia

Resumen Existen pocos reportes de la prevalencia de parásitos gastrointestinales en equinos en Colombia. En este estudio se muestrearon 946 caballos de un total de 204 predios en los municipios de Rionegro y los que comprenden el Valle de Aburrá departamento de Antioquia. La materia fecal fue tomada directamente del recto y procesada por las técnicas de McMaster׳ Baermann y Graham. La prevalencia de parásitos a nivel de predios fue del 563%׳ para Strongylidae׳ 7%׳10 para Strongyloides spp8%׳2 ׳ para Oxyuridae y 03%׳ para Dictyocaulus spp. Desde el punto de vista práctico׳ los más patogénicos y de mayor problema de resistencia son los Cyathostomidos׳ que se incluyen dentro de los Strongylidae y cuya prevalen-cia fue superior al 50%. El OR de presentar Strongylidae es 49% menor en equinos que participan en ferias en comparación con los que no lo hacen. El OR de presentar Strongylidae es 56% menor en equinos que usan pesebrera en comparación con los que no la usan. Por su parte׳ el OR de ser positivo a Oxyuris equi es 88% menor en animales que salen a ferias en comparación con los que no salen a feria y el OR de ser positivo a Oxyuris equi es 366׳ veces mayor en equinos con condición corporal <2 en comparación con equinos de condición corporal >3. Con base en esta información es importante iniciar estudios de resistencia a antihelmínticos y evaluar la relación entre la carga parasitaria y las posibles afecciones clínicas para así determinar el impacto real de estas parasitosis en los equinos de estas regiones.

Abstract There are few reports on the prevalence of gastrointestinal parasites in equines in Colombia. In this study946 ׳ horses were sampled from a total of 204 farms in the municipalities of Rionegro and those that comprise the Valle de Aburrá׳ in the department of Antioquia. Fecal samples were taken directly from the rectum and processed by the McMaster׳ Baermann and Graham techniques. The prevalence of parasites at the farm level was 56.3% for Strongyüdae10.7% ׳ for Strongyloides spp2.8% ׳ for Oxyuridae and 0.3% for Dictyocaulus spp. From a practical point of view׳ the most pathogenic parasites and of greatest resistance concern were the Cyathostomids׳ which are included within the Strongylidae and whose prevalence was greater than 50%. The OR of presenting Strongylidae was 49% lower in equines that participated in fairs compared to those that did not. The OR of presenting Strongylidae was 56% lower in equines that use stables than those not being stalled. A1so׳ the OR of being positive to Oxyuris equi was 88% lower in animals that participate in fairs compared to those that did not׳ and was 3.66 times higher in equines with body condition < 2 in comparison with those of body condition >3. Based on this information it is important to initiate studies that evaluate the state of antihelminthic resistance and the relationship between parasitic load and clinical condition in order to determine the real impact of these parasites on the equines of these regions.

Resumo Existem poucos reportes da prevalência de parasitos gastrointestinais em equinos na Colômbia. Nessa pesquisa foram amostrados 946 cavalos de um total de 204 fazendas nos municípios de Rionegro e dos que formam o Vale de Aburrá׳ no departamento de Antióquia. A matéria fecal foi tirada diretamente do reto e processada pelas técnicas de McMaster׳ Baermann e Graham. A prevalência de parasitos das fazendas foi de 56.3% para Strongylidae7%׳10 ׳ para Strongyloides spp8%׳2 ׳ para Oxyuridae y 0.3% para Dictyocaulus spp. Desde o ponto de vista prático׳ os mais patogênicos e de maior problema de resistência são os Cyathostomidos׳ que estão incluídos dentro dos Stronglylidae e cuja prevalência foi superior ao 50%. O OR de apresentar Strongylidae é 49% menor nos equinos que participam em férias comparado com os que não. O OR de apresentar Strongylidae é 56% menor nos equinos que usam manjedoura comparado com os que não a usam. Por outro lado׳ o OR de ser positivo a Oxyuris equi é 88% menor em animais que vão a férias comparado com os que não vão e o OR de ser positivo a Oxyuris equi é 3.66 vezes maior nos equinos com condição corporal <2 comparado com os equinos de condição corporal >3. Com base nesta informação׳ se conclui que é importante iniciar estudos de resistência a anti-helmínticos e avaliar a relação entre a carga parasitária e as possíveis afecções clínicas para assim determinar o impacto real destas parasitoses nos equinos destas regiões.

Point prevalence of gastrointestinal parasites in double purpose cattle of Rio de Oro and Aguachica municipalities, Cesar state, Colombia.

Gastrointestinal parasites are one of the most important health problems in cattle worldwide, as they cause economic losses in the herds. Twenty - seven double purpose herds were visited to determine the prevalence of gastrointestinal parasites in cattle at the Rio de Oro and Aguachica municipalities. Overall, 862 fecal samples were collected in three age groups: <12months, 12-24months and >24months. Stool samples were taken directly from the rectum and refrigerated until processing. For parasite determination, fecal samples were processed using coprological techniques. The parasitic genera were identified by egg or infective larval morphology. The global prevalence of gastrointestinal parasites was 83.2%, being the highest values for Eimeria sp. (77.9%), Strongyloides sp. (10.8%) and Haemonchus sp. (8.5%). Regarding the prevalence by municipalities, there was no statistical association (P>0.05) indicating that the prevalence was similar in the two municipalities. Regarding the results for Eimeria sp., different degrees of positivity were observed, reflecting that there was statistical association (P<0.05) with respect to the age group, suggesting that adult animals may act as infection source for calves. Likewise, there was statistical association (P<0.05) between the prevalence for Strongyloides sp., Haemonchus sp. and Trichostrongylus sp. with respect to the age group. It is concluded that there is a high prevalence of gastrointestinal parasites in dual-purpose cattle in the municipalities under study, and that it could be controlled by improving the hygienic conditions of the herds, and informing the farmer about parasite control programs.