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1.
Org Biomol Chem ; 9(5): 1547-54, 2011 Mar 07.
Article in English | MEDLINE | ID: mdl-21234463

ABSTRACT

The triflic acid-mediated endocyclic N-acyliminium ion cyclisation provides a facile synthesis of (di)-benzazocinones. On reduction of the 10-phenyl derivative, an unusually non-polar tertiary alkylamine was obtained.


Subject(s)
Benzene/chemistry , Cyclazocine/chemical synthesis , Imines/chemistry , Acylation , Crystallography, X-Ray , Cyclization , Ions/chemistry , Models, Molecular , Molecular Structure
2.
Dalton Trans ; (23): 4458-67, 2009 Jun 21.
Article in English | MEDLINE | ID: mdl-19488443

ABSTRACT

Reactions of M(2)(CO)(10) (M = Re, Mn) with pyrimidine-2-thiol (pymSH) in the presence of Me(3)NO afford the tetranuclear square-type complexes [M(4)(CO)(12)(micro-kappa(3)-pymS)(4)] (, M = Re; , M = Mn). Both consist of four M(CO)(3) (M = Re, Mn) units, pairs of which are joined by tridentate pyrimidine-2-thiolate ligands. Treatment of with a variety of donor ligands results in cleavage of the square to afford mononuclear species with either a mono- or bidentate pyrimidine-2-thiolate ligand. Triphenylphosphine reacts with to give [Mn(CO)(3)(PPh(3))(kappa(2)-pymS)] () in which the pyrimidine-2-thiolate coordinates in a bidentate fashion. With diamines [M(CO)(3)(kappa(2)-L)(kappa(1)-pymS)] () (M = Re, Mn; L = 2,2'- bipy, 1,10-phen, en) result in which the pyrimidine-2-thiolate binds in a monodentate fashion through sulfur. With diphosphines, complexes with different stoichiometries and pyrimidine-2-thiolate binding modes are obtained depending on the nature of the metal and diphosphine. With dppm and dppe, gives [Re(CO)(2)(kappa(1)-pymS)(kappa(2)-dppm)] () and [Re(CO)(2)(kappa(2)-pymS)(kappa(1)-dppe)(2)] (), respectively, whereas affords [Mn(CO)(2)(kappa(2)-pymS)(kappa(1)-dppm)(2)] () and [Mn(CO)(2)(kappa(2)-pyS)(kappa(2)-dppe)] () under similar conditions. Reactions of with [Os(3)(CO)(10)(NCMe)(2)] affords mixed-metal butterfly clusters [MOs(3)(CO)(13)(micro(3)-kappa(2)-pymS)] () in which the group 7 metal occupies a wing-tip position and the pyrimidine-2-thiolate ligand caps a triangular Os(2)M face. With Ru(3)(CO)(12), carbon-sulfur bond cleavage occurs to give the tetranuclear clusters [MRu(3)(CO)(14)(micro(4)-S)(micro-kappa(1):eta(1)-pym)] () bearing both the extruded sulfur and the heterocyclic ring. The molecular structures of , and have been established by X-ray diffraction allowing the binding mode of the pyrimidine-2-thiolate ligands to be probed.

3.
J Med Chem ; 41(4): 451-67, 1998 Feb 12.
Article in English | MEDLINE | ID: mdl-9484496

ABSTRACT

Tetrahydrocyclopent[b]indoles, tetrahydrocarbazoles, and hexahydrocyclohept[b]indoles have been prepared as melatonin analogues to investigate the nature of the binding site of the melatonin receptor. The affinity of analogues was compared in a radioligand binding assay using chicken brain membranes and agonist and antagonist potency measured in clonal Xenopus laevis melanophore cells. Comparison of the N-acyl-3-amino-6-methoxytetrahydrocarbazoles (2) with N-acyl-4-(aminomethyl)-6-methoxy-9-methyltetrahydrocarbazoles (9) showed that the latter have much higher binding affinities for the chicken brain receptor. Comparison of N-acyl-1-(aminomethyl)-7-methoxy-4-methyltetrahydrocyclopent[b]ind oles (10), 6-methoxytetrahydrocarbazoles (9), and N-acyl-10-(aminomethyl)-2-methoxy-5-methylhexahydrocyclohept[b]ind oles (11) showed that the tetrahydrocarbazoles had the highest binding affinity with the cyclohept[b]indoles and the cyclopent[b]indoles having rather lower affinities. All of these observations are in agreement with our postulated model of melatonin orientation at the binding pocket in which the 3-amidoethane side chain is in a conformation close to the 5-methoxyl group, as is shown in the X-ray crystallographic structure of 9m and in the energy-minimized computed structures. Separation of the enantiomers of members from each of these three systems was accomplished by chiral HPLC. It was found that in all cases the (-)-enantiomer had a higher binding affinity than the (+)-enantiomer. An X-ray crystallographic analysis of the two enantiomers of 9a showed that the (+)-enantiomer had the (R) absolute stereochemistry. Since the sign of the Cotton curves, determined from circular dichroism studies, was the same for all (+)-enantiomers, it is assumed that the absolute stereochemistry at these centers is identical. In the Xenopus melanophore assay, the tetrahydrocarbazoles 2 (R = H) were mainly weak antagonists, while those with R = OMe were agonists. The biological behavior of the tetrahydrocarbazoles 9 (R = H) depended on R1, some being agonists and some antagonists, whereas those with R = OMe were generally agonists. Variation of the R and R1 groups in compounds of type 9 produced both agonists and antagonists. The tetrahydrocylopentaindoles 10 had similar biological properties to the corresponding analogues of 9, but the hexahydrocycloheptaindoles 11 showed a much greater propensity to be antagonists. In all cases the (S)-enantiomers were found to be more potent agonists than the (R)-enantiomers.


Subject(s)
Carbazoles/chemical synthesis , Indoles/chemical synthesis , Receptors, Cell Surface/agonists , Receptors, Cell Surface/antagonists & inhibitors , Receptors, Cytoplasmic and Nuclear/agonists , Receptors, Cytoplasmic and Nuclear/antagonists & inhibitors , Animals , Binding, Competitive , Brain/metabolism , Carbazoles/chemistry , Carbazoles/pharmacology , Cell Membrane/metabolism , Chickens , Crystallography, X-Ray , Indicators and Reagents , Indoles/chemistry , Indoles/pharmacology , Melanophores/cytology , Melanophores/drug effects , Melanophores/physiology , Melatonin/metabolism , Models, Molecular , Molecular Conformation , Receptors, Melatonin , Structure-Activity Relationship , Xenopus laevis
4.
J Med Chem ; 38(7): 1132-9, 1995 Mar 31.
Article in English | MEDLINE | ID: mdl-7707316

ABSTRACT

Three series of 2-phenyltryptamides were prepared as melatonin analogues to investigate the nature of the binding site of the melatonin receptor in chicken brain and in Xenopus laevis melanophore cells. The 5-methoxy-2-phenyltryptamides (6a-j) have high binding affinities for the chicken brain receptor, in some cases (6a-d) greater than that for melatonin, confirming and extending the work of Spadoni et al., and act as agonists in the Xenopus melanophore assay. Analogues lacking the 5-methoxyl group (2a-n) had a considerably lower affinity for the chicken brain receptor. In the Xenopus melanophore assay the compounds acylated on nitrogen by an alkyl group (2a-d) were agonists whereas the compounds acylated on nitrogen by an alicyclic group (2f-i) were antagonists. Introducing a methyl group at N1 (7) led to an increase in binding affinity in the chicken brain assay, whereas introducing an ethyl group (13) led to a decrease in binding affinity. A methyl substituent at the beta-position of the 3-amidoethane side chain (8, 11) also led to an increase in the binding affinity. The only analogue acylated on nitrogen with an alkyl group (acetyl) which showed antagonist activity was 9, which has a beta-methoxymethyl side chain. In the absence of the 5-methoxyl group the methoxymethyl function may cause the molecule to bind in a different configuration so that it is no longer able to activate the receptor. All of these observations are in agreement with a model of melatonin at the receptor site in which the 3-amidoethane side chain is in a conformation close to the 5-methoxyl group.


Subject(s)
Receptors, Cell Surface/agonists , Receptors, Cell Surface/antagonists & inhibitors , Tryptamines/metabolism , Animals , Binding Sites , Chickens , Drug Design , Ligands , Melanophores , Melatonin/chemistry , Molecular Structure , Radioligand Assay , Receptors, Melatonin , Structure-Activity Relationship , Tryptamines/chemical synthesis , Xenopus laevis
5.
Anticancer Drug Des ; 7(1): 3-14, 1992 Feb.
Article in English | MEDLINE | ID: mdl-1543526

ABSTRACT

A new transition metal complex of the 5-nitroimidazole, metronidazole (1-beta-hydroxyethyl-2-methyl-5-nitroimidazole), has been prepared and its potential use as a hypoxic cell cytotoxic agent examined. The preparation of the complex [(eta6-C6H6)RuCl2(metronidazole)] is described together with its characterization using standard spectroscopic techniques. Electrochemical investigations showed that coordination to the metal centre had not altered the electron affinity of the metronidazole, but kinetic studies using the cyclic voltametric mode demonstrated that the one-electron addition product, the nitro radical anion, had a decreased lifetime, with a half-life of 7.75 and 11.9 s for the coordinated and free metronidazole ligand respectively. Biological studies employed viscosity measurements, DNA SOS repair capacity and a transfection assay to examine the effect on DNA. Conductance studies were also employed to determine the influence on intact Escherichia coli growth rates. The ruthenium-metronidazole complex showed greater activity than metronidazole aerobically, but a higher differential activity under hypoxic reduction conditions, due to activation of the NO2 group. Results with intact cells suggested a greater selective cytotoxicity with metronidazole coordinated to ruthenium than attained with the free ligand.


Subject(s)
Antineoplastic Agents/chemical synthesis , DNA Damage , Metronidazole/analogs & derivatives , Organometallic Compounds/chemical synthesis , SOS Response, Genetics/drug effects , Antineoplastic Agents/toxicity , Drug Design , Electrochemistry , Escherichia coli/drug effects , Escherichia coli/genetics , Metronidazole/chemical synthesis , Metronidazole/toxicity , Molecular Structure , Organometallic Compounds/toxicity , Transfection
6.
Free Radic Res Commun ; 14(1): 33-40, 1991.
Article in English | MEDLINE | ID: mdl-2022344

ABSTRACT

The electrochemical behaviour of three nitrofuran compounds, nitrofurazone, nitrofurantoin and furazolidone, has been studied in three solvent types; aprotic, aqueous and mixed, and at four working electrodes. Particular attention has focused on the 1-electron RNO2/RNO2.- couple as measured by the cyclic voltammetric mode. Using Hg in aqueous buffer, reduction of the NO2 group proceeds directly to the hydroxylamine with no intermediate stages being identified. Addition of an aprotic solvent gave a 2-stage reduction, initially forming the RNO2.- species. At all solid electrodes, however, the RNO2/RNO2.- couple was identified under simple aqueous conditions. The switch to a mixed aqueous/aprotic solvent medium produced only minor changes in the response compared with the situation on Hg. This presents the opportunity of using nitrofuran complexes as model systems for the redox behaviour of nitro aromatic compounds in general at solid electrode surfaces where the latters' more negative reduction potentials makes direct study difficult. The conditions have been defined whereby we can examine pH effects and RNO2.- biological target interactions in simple aqueous media to allow the further refinement of the electrolytic model system for studying bio-reducible drug action.


Subject(s)
Furazolidone/chemistry , Nitrofurantoin/chemistry , Nitrofurazone/chemistry , Carbon , Electrochemistry , Electrodes , Free Radicals , Gold , Mercury , Oxidation-Reduction , Platinum , Solvents
7.
Anticancer Drug Des ; 4(4): 295-302, 1989 Dec.
Article in English | MEDLINE | ID: mdl-2619867

ABSTRACT

A novel bifunctional hypoxia-selective compound [Rh2(O2CCH3)4.2C6N3O3H9] has been synthesized and its genotoxic and potential mutagenic effects studied with reference to those of dirhodium tetraacetate (RAc) and metronidazole. The properties of the two functional components have been examined by comparing its oxic genotoxicity, a measure of the DNA damage induced by RAc, with its anoxic genotoxicity by electrochemical reduction, a measure of DNA damage resulting from the combined activity of reduced nitro group intermediates and RAc. The induction of DNA SOS repair has also been studied as well as the strand-breaking ability of the compound using viscometry. The genotoxic effects observed are proportional to the drug concentration over the range tested and the compound exhibits a high selective toxicity differential to hypoxic bacteria. The strand-breaking and mutagenic properties are governed by the metronidazole component and other effects, such as inhibition of DNA synthesis, are governed by the RAc component.


Subject(s)
DNA Damage , DNA Repair , DNA/drug effects , Metronidazole/pharmacology , Rhodium/pharmacology , SOS Response, Genetics , Oxidation-Reduction , Viscosity
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