ABSTRACT
Immunohistochemical examination of the proliferative activity of cells was made investigating the expression of Ki-67 protein and the location of proteins associated with epithelial cell papillomavirus infection involving P16(INK4a) and HPV16 proteins in different cell areas of the intact mucosa, in leukoplakia with the signs of hyperplasia and dysplasia, and in squamous cell carcinoma. There was a positive correlation between the proliferative activity of cells in the parabasal cell areas and the expression of P16(INK4a) protein in oral leukoplakia with the signs of hyperplasia (r(s)=0.397; p=0.018). In oral leukoplakia with dysplastic changes, there was a positive correlation between the proliferation of cells in the parabasal and prickle cell layers and the location of HPV type 16 antigens (r(s)=0.515; p=0.041 and r(s)=0.651; p=0.006). Detection of papillomavirus infection in leukoplakia can solve not only the problems with its genesis, but this is also a morphological basis for the effective prevention and treatment of this common oral mucosal disease.
Subject(s)
Carcinoma, Squamous Cell/genetics , Leukoplakia, Oral/pathology , Leukoplakia, Oral/virology , Mouth Neoplasms/genetics , Papillomaviridae/pathogenicity , Adult , Aged , Antigens, Viral/isolation & purification , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/virology , Cyclin-Dependent Kinase Inhibitor p16/biosynthesis , DNA, Viral/isolation & purification , Female , Human papillomavirus 16/immunology , Human papillomavirus 16/isolation & purification , Humans , Ki-67 Antigen/biosynthesis , Leukoplakia, Oral/etiology , Male , Middle Aged , Mouth Mucosa/pathology , Mouth Neoplasms/pathology , Mouth Neoplasms/virology , Papillomaviridae/isolation & purificationABSTRACT
The correlation was investigated between the level of cell proliferation and the intensity of claudin-1 expression in oral hyperplasia, squamous intraepithelial neoplasia (SIN) and squamous cell carcinoma. Claudin-1 expression and cell proliferation were assessed using immunohistochemistry in different epithelial layers of oral mucosa. The inverse correlation was revealed between the level of cell proliferation and the intensity of claudin-1 expression in oral hyperplasia, SIN and squamous cell carcinoma.
Subject(s)
Carcinoma in Situ/pathology , Carcinoma, Squamous Cell/pathology , Claudin-1/metabolism , Ki-67 Antigen/metabolism , Mouth Mucosa/pathology , Mouth Neoplasms/pathology , Adult , Carcinoma in Situ/metabolism , Carcinoma, Squamous Cell/metabolism , Cell Proliferation , Female , Humans , Hyperplasia/metabolism , Hyperplasia/pathology , Male , Mouth Mucosa/metabolism , Mouth Neoplasms/metabolismSubject(s)
Leukoplakia/pathology , Leukoplakia/therapy , Female , Humans , Leukoplakia/classification , MaleABSTRACT
The problem of diagnostic of various forms of leukoplakia of the oral mucosa is reviewed IT is introduced a set of methods for diagnosis of the disease, including a clinical check up, evaluation of the data of optical coherence tomography, classical histological and immunohistochemical study to determine the neoplastic cell transformation of oral mucosa in the early stages of its development.
Subject(s)
Leukoplakia, Oral/classification , Leukoplakia, Oral/pathology , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Tomography, Optical CoherenceABSTRACT
The study included 32 patients (among them 20 patients with simple and 12 with verrucous leukoplakia) and presents both classic and advanced diagnostic methods for this disease: clinical examination, histological and immunohistochemical studies. The latter allows revealing neoplastic transformation of the oral mucosa.
