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1.
Tsitologiia ; 46(6): 525-30, 2004.
Article in Russian | MEDLINE | ID: mdl-15341128

ABSTRACT

For the first time, it has been shown that population of proteasomes is heterogeneous in their RNAse activity. EGF exerts selective effect on different subpopulations of proteasomes. The RNAse activity of cytoplasmic proteasomes is induced under the influence of EGF on epidermoid carcinoma cell line A431. However, the activity of proteasomes isolated from culture medium and of nuclear proteasomes is inhibited by EGF. The above enzymatic activity has been shown to be specifically and selectively dependent on phosphorylation of proteasomal subunits in different subpopulations of proteasomes. Proteasome involvement in the coordinated control of specific messenger RNA molecules stability is suggested, and one of the mechanisms of this control might be an export of specific subpopulation of proteasomes from the cell.


Subject(s)
Cell Nucleus/enzymology , Cytoplasm/enzymology , Endoribonucleases/metabolism , Epidermal Growth Factor/pharmacology , Peptide Hydrolases/metabolism , Proteasome Endopeptidase Complex , Cell Line, Tumor , Cell Nucleus/drug effects , Cytoplasm/drug effects , Humans , Phosphorylation/drug effects , Protease Inhibitors/pharmacology , RNA, Messenger/metabolism
2.
Tsitologiia ; 46(3): 283-90, 2004.
Article in Russian | MEDLINE | ID: mdl-15214174

ABSTRACT

It has been shown that endoribonuclease activity of alpha-RNP particles and 26S proteasomes are changed under the action of inductors of programmed cell death. Treatment of K562 cells with inductors of apoptosis--doxorubicin (adriamycin) and diethylmaleate--lead to a significant stimulation of RNAse activity of alpha-RNP and to reduction of proteasome RNase activity. The enzymatic activity under study has been shown to be specifically and selectively dependent on phosphorylation of subunits of alpha-RNP particles and 26S proteasomes. The characteristics of RNAse activity of different subpopulations of proteasomes differ. The specificity of a subpopulation of proteasomes exported from the cell has been demonstrated. Proteasome and alpha-RNP involvement in the coordinated control of stability of various specific messenger RNA molecules is suggested, and one of the mechanisms of this control might be the export of specific subpopulation of proteasomes from the cell.


Subject(s)
Apoptosis/physiology , Endoribonucleases/metabolism , Peptide Hydrolases/metabolism , Proteasome Endopeptidase Complex , RNA Stability , Ribonucleoproteins, Small Cytoplasmic/metabolism , Cell Line, Tumor , Doxorubicin/pharmacology , Humans , Maleates/pharmacology , Phosphorylation , RNA, Messenger/metabolism , Species Specificity
3.
Tsitologiia ; 45(5): 488-92, 2003.
Article in Russian | MEDLINE | ID: mdl-14521056

ABSTRACT

For the first time it has been shown that RNase activity is induced under the influence of EGF on epidermoid carcinoma cell line A431. Proteasomes from EGF-treated A431 cells destabilize the 3'-untranslated regions of non-muscle beta actin mRNA, creating a specific cleavage pattern. In addition, these particles have been shown to specifically cleave Alu-containing informational RNA. The enzymatic activity under study has been shown to be dependent on phosphorylation of proteasomal subunits and specifically and selectively regulated by Ca and Mg ions. Proteasome involvement in the coordinated control of stability of specific messenger RNA molecules is suggested. The endoribonuclease activity of 26S proteasomes can constitute a link between EGF signaling pathways and RNA stability.


Subject(s)
Endoribonucleases/metabolism , Epidermal Growth Factor/pharmacology , Peptide Hydrolases/drug effects , Proteasome Endopeptidase Complex , RNA, Messenger/metabolism , 3' Untranslated Regions/metabolism , Actins/genetics , Aluminum , Calcium Chloride , Cell Line, Tumor , Humans , Magnesium Chloride , Peptide Hydrolases/metabolism , Phosphorylation , RNA, Messenger/chemistry , Signal Transduction
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