ABSTRACT
OBJECTIVE: The present study compared three models of induction of osteoarthritis (OA) and rheumatoid arthritis (RA) in the temporomandibular joint (TMJ) of rats. DESIGN: The induction method was by injection of complete Freund's adjuvant (CFA) + type II bovine collagen (CII). Twenty-four adult male rats were divided into four groups (n = 6): G1: Sham, 50 µL of 0.9% sodium chloride at the base of the tail and in each TMJ; G2: OA, 50 µL CFA+CII in each TMJ; G3: RA+OA, 100 µL of CFA+CII at the base of the tail and 50 µL CFA+CII in each TMJ; G4: RA, 100 µL of CFA+CII at the base of the tail. All injections were repeated 5 days later. Twenty-three days after the first injection, the animals were sacrificed and the TMJs were submitted to histomorphometric analysis and measurement of cytokines. The Kruskal-Wallis and Dunn tests were used (alpha=0.05). RESULTS: The total thickness of the condylar cartilage increased in G2 in relation to the other groups, G3 and G4 reduced in relation to G1; and G2 and G4 reduced in relation to G2 and G3. The levels of IL-1ß, IL-6 and TNF-α increased in the three induction models compared to G1. The level of IL-10 increased in G2 compared to the other groups and reduced in G3 and G4 compared to G1. CONCLUSION: CFA+CII induced inflammation and degeneration compatible with RA (advanced chronic stage) when injected in the tail, and compatible with OA (acute stage or early disease) when injected only in the TMJ.
Subject(s)
Arthritis, Rheumatoid , Osteoarthritis , Temporomandibular Joint Disorders , Rats , Male , Animals , Cattle , Temporomandibular Joint Disorders/chemically induced , Temporomandibular Joint , Osteoarthritis/chemically induced , Freund's AdjuvantABSTRACT
We report the synthesis and characterization of two novel tetra-cationic porphyrins, containing Pt(II) or Pd(II) polypyridyl complexes attached at the peripheral position of N4-macrocycle. Compounds were characterized through elemental analysis, molar conductivity, cyclic voltammetry, and spectroscopy analysis. Photophysical and photobiological parameters were also evaluated. Also, the binding capacity of each porphyrin with human serum albumin (HSA) was determined by UV-Vis, steady-state, and time-resolved fluorescence spectroscopy, combined with molecular docking calculations. The results suggest that the interaction of these compounds is spontaneous, weak to moderate, and probably occurs at site III (subdomain IB) by non-covalent forces, including van der Waals and H-bonding. Moreover, porphyrins containing peripheral complexes improve their interactions with biomolecules, show good photostability, generate reactive oxygen species under white light studied by electron paramagnetic resonance (EPR) analysis, and promote photo-damage of HSA.
Subject(s)
Palladium/pharmacology , Platinum Compounds/pharmacology , Porphyrins/pharmacology , Electron Spin Resonance Spectroscopy , Humans , Models, Molecular , Molecular Docking Simulation , Molecular Structure , Palladium/chemistry , Photochemotherapy , Photosensitizing Agents , Platinum Compounds/chemistry , Porphyrins/chemistry , Protein Conformation , Serum Albumin/chemistryABSTRACT
O presente relato de caso clínico tem o objetivo de descrever a resolução cirúrgica de um dente clinicamente assintomático, portador de fístula muco-gengival, com imagem radiográfica sugestiva de cisto radicular com provável presença de cálculo aderido à região apical. O tratamento empregado foi intervenção endodôntica convencional com medicação intracanal prolongada de pasta de hidróxido de cálcio/soro fisiológico, obturação definitiva seguida de intervenção cirúrgica paraendodôntica (apicectomia). O material curetado foi enviado para exame histopatológico, confirmando a hipótese diagnóstica de cisto radicular. Já o fragmento do ápice radicular envolto por massa consistente foi analisado em microscopia eletrônica de varredura, demonstrando se tratar de cálculo dental aderido ao cemento e à dentina radicular. Após período de 9 meses e 21 dias o elemento dental se apresentou clínica e radiograficamente em processo de reparo apical.
The present report of clinical case has the objective of describe the surgical resolution of a tooth clinically asymptomatic that has muco-gingíval fistula with suggestive radiographic image of root cist and a probable presence of calculus adhered to the apical region. The treatment applied was conventional endodontic intervention with a prolonged intracanal medicine with hydroxide calcium/normal saline paste, definite filling followed by a paraendodontic surgical intervention. The curetted material was sent to a histopatologic exam, confirming the diagnosis hypothesis of radicular cist. The fragment of the root apice involved by a solid substance was analized by an eletronic microscopy of scanning, probably demonstrating to be about dental calculus adhered to the cementum and to the root dentin. After a period of 9 months and 21 days, the dental element showed to be clinical and radiographically in an apical repair processo.
Subject(s)
Humans , Male , Female , Radicular Cyst/diagnosis , Endodontics/methods , Periapical Periodontitis/diagnosis , Dental Plaque/diagnosisABSTRACT
O autor relata a sua experiência com o acetato de guanabenzo no tratamento de pacientes hipertensos leves e moderados. Os pacientes receberam, durante 60 dias, uma dose de acetato de guanabenzo que variou de 8 a 32 mg/dia, aumentada progressivamente e de acordo com a necessidade de cada caso, sempre em duas tomadas (manhä e noite), sendo que a maioria atingiu níveis pressóricos satisfatórios com 8 mg/dia. Uma avaliaçäo clínica final apresenta os seguintes resultados: 73,4% de casos considerados satisfatórios; 6,6% de casos considerados intermediários e 20,0% de casos onde o resultado deve ser considerado näo satisfatório. O medicamento mostrou-se eficaz e seguro, de posologia cômoda e tolerância muito boa, além de reduzir os níveis séricos de colesterol e glicose
Subject(s)
Humans , Guanabenz/therapeutic use , Hypertension/drug therapy , Clinical Trials as TopicABSTRACT
O autor relata a sua experiência com o acetato de guanabenzo no tratamento de pacientes hipertensos leves e moderados. Os pacientes receberam, durante 60 dias, uma dose de acetato de guanabenzo que variou de 8 a 32 mg/dia, aumentada progressivamente e de acordo com a necessidade de cada caso, sempre em duas tomadas (manhä e noite), sendo que a maioria atingiu níveis pressóricos satisfatórios com 8 mg/dia. Uma avaliaçäo clínica final apresenta os seguintes resultados: 73,4% de casos considerados satisfatórios; 6,6% de casos considerados intermediários e 20,0% de casos onde o resultado deve ser considerado näo satisfatório. O medicamento mostrou-se eficaz e seguro, de posologia cômoda e tolerância muito boa, além de reduzir os níveis séricos de colesterol e glicose