ABSTRACT
ABSTRACT Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromIDTM VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95.52% but a low specificity of 30%.
Subject(s)
Humans , Bacteriological Techniques/methods , Gram-Positive Bacterial Infections/microbiology , Culture Media/chemistry , Vancomycin-Resistant Enterococci/growth & development , Vancomycin-Resistant Enterococci/drug effects , Microbial Sensitivity Tests , Bacteriological Techniques/instrumentation , Culture Media/metabolism , Feces/microbiology , Vancomycin-Resistant Enterococci/metabolismABSTRACT
Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromID™ VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95.52% but a low specificity of 30%.
Subject(s)
Bacteriological Techniques/methods , Culture Media/chemistry , Gram-Positive Bacterial Infections/microbiology , Vancomycin-Resistant Enterococci/growth & development , Vancomycin-Resistant Enterococci/isolation & purification , Bacteriological Techniques/instrumentation , Culture Media/metabolism , Feces/microbiology , Humans , Microbial Sensitivity Tests , Vancomycin-Resistant Enterococci/drug effects , Vancomycin-Resistant Enterococci/metabolismABSTRACT
The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of Brazil. Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome.
