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1.
Steroids ; 140: 131-143, 2018 12.
Article in English | MEDLINE | ID: mdl-30315840

ABSTRACT

Synthetic transformations of steroids for drug discovery and improvement of drug effectiveness have been an important part of modern medicinal chemistry and pharmaceutical sciences. Pentacyclic triterpenoids, being represented in the nature by various structures and biogenetically related to steroids, can largely expand the spectrum of biologically active steroidal agents via synthesis of the so-called "triterpenoid-steroid" hybrids. In the presented work, the nitrile anion cyclizations of 3,4-secolupane and 3,4-seco-oleanane nitriles and follow-up synthetic transformations of the cyclized products with formation of the gemm-dimethyl-free A ring "triterpenoid-steroid" hybrids were studied. Furthermore, the resulting cyclic compounds were modified at C3, C4, and/or C5 positions of ring A, as well as at C20, C28, and C30 positions of the isopropylidene moiety in the case of lupane triterpenoids. The cytotoxic effect of the synthesized compounds against seven cancer cell lines HEp-2, HCT 116, MS, RD TE32, A549, MCF7, and PC3 was evaluated. The in silico identification of potential anticancer protein targets with regard to the compounds, which were active at micromolar concentrations against tested cell lines, was carried out. The molecular docking studies showed that compound 19, which demonstrated most pronounced cytotoxicity (IC50 0.64-3.17 µM) against all tested cell lines, fits well the active sites of CDK6 and HER2/neu.


Subject(s)
Molecular Docking Simulation , Steroids/chemistry , Triterpenes/chemistry , Triterpenes/pharmacology , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Antineoplastic Agents/metabolism , Antineoplastic Agents/pharmacology , Catalytic Domain , Cell Line, Tumor , Cyclin-Dependent Kinase 6/chemistry , Cyclin-Dependent Kinase 6/metabolism , Humans , Receptor, ErbB-2/chemistry , Receptor, ErbB-2/metabolism , Structure-Activity Relationship , Triterpenes/chemical synthesis , Triterpenes/metabolism
2.
Eur J Med Chem ; 140: 74-83, 2017 Nov 10.
Article in English | MEDLINE | ID: mdl-28923388

ABSTRACT

Extremely low content of biologically active triterpenoids with the fragmented or contracted ring A extractable from plants is the main disadvantage of their use in drug discovery and practical pharmacology. Development of new methods for synthesis of these compounds and their structural analogs from bioavailable triterpene precursors gives an opportunity to obtain promising agents for pharmacology with excellent yields. A new approach to synthesis of alkylated A-seco-triterpenoids, including the Beckmann fragmentation of 3-methyl-substituted allobetulin or betulinic acid methyl ester with 2-hydroxyimino group in the ring A was proposed. These compounds were used to prepare a series of 2,3-seco- and five-membered ring A lupane and oleanane derivatives, cytotoxicity of which was screened in vitro against the cancer (HEp-2, HCT 116, A549, RD TE32, MS) and non-cancerous (HEK 293) cell lines. Methyl 3-bromomethyl-1-cyano-3-oxo-2,3-seco-2-norlup-20(29)-en-30-al-28-oate was selected as the most active compound (IC50 3.4-10.4 µM for HEp-2, HCT 116, RD TE32, MS cells) capable of triggering caspase-8-mediated apoptosis in HCT 116 cells accompanied by typical apoptotic chromatin condensation, without any loss of mitochondrial membrane permeability.


Subject(s)
Antineoplastic Agents/pharmacology , Secosteroids/pharmacology , Alkylation , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Cell Proliferation/drug effects , Cell Survival/drug effects , Cells, Cultured , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Molecular Structure , Secosteroids/chemical synthesis , Secosteroids/chemistry , Structure-Activity Relationship
3.
Eur J Med Chem ; 125: 629-639, 2017 Jan 05.
Article in English | MEDLINE | ID: mdl-27721148

ABSTRACT

An efficient scheme to synthesize novel ring-A fused heterocyclic derivatives of betulin was developed. The starting reaction of this synthesis was one-pot selective bacterial oxidation of betulin to betulone used as the key compound to synthesize the substituted azoles such as C(2)-C(3)-fused 1,2,3-triazoles, oxazoles and 1,2,4-triazine, as well as C(1)-C(2)-fused isoxazoles. The semi-synthetic compounds were screened for their cytotoxic activity against human cancer cell lines A549, HCT 116, HEp-2, MS and RD TE32 with use of the photometric MTT assays. Among the tested compounds, N-acetyltriazole of betulin (10) displayed impressive cytotoxic activity with IC50 2.3-7.5 µM against HCT 116, HEp-2, MS and RD TE32 cell lines as well as 3-methyl-4-oxido-1,2,4-triazine-derivative of betulonic acid (12) that was active against HCT 116 and HEp-2 cell lines with IC50 1.4 and 1.5 µM, respectively. Comparative experiments showed triazole (10) to have a lower cytotoxicity to normal epithelial cells, in comparison with compound (12). In accord with the in vivo acute toxicity test, the LD50 of triazole (10) exceeded 600 mg/kg. The ability of the most potent active triazole (10) to trigger apoptotic cell death was explored in the Annexin V-FITC test and by analyzing of caspase activity and morphological alterations in mitochondria and nuclei of HCT 116 cells.


Subject(s)
Azoles/chemical synthesis , Azoles/toxicity , Triterpenes/chemistry , Azoles/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Humans , Lethal Dose 50 , Molecular Structure , Triazines/chemistry , Triazines/toxicity , Triterpenes/toxicity
4.
Eur J Med Chem ; 83: 601-8, 2014 Aug 18.
Article in English | MEDLINE | ID: mdl-24997292

ABSTRACT

Triterpene derivatives with an α,ß-alkenenitrile moiety in the five-membered ring A have been synthesized by nitrile anion cyclizations of 1-cyano-2,3-secotriterpenoids. Oxime-containing precursors, 2,3-secointermediates and five-membered ring A products of cyclizations were screened for in vitro antiviral activity against enveloped viruses - influenza A virus and human immunodeficiency virus type I (HIV-1). Lupane ketoxime and the 2,3-secolupane C-3 aldoxime which possess antiviral activities against both influenza A virus (EC50 12.9-18.2 µM) and HIV-1 (EC50 0.06 µM) were the most promising compounds.


Subject(s)
Antiviral Agents/chemistry , Antiviral Agents/pharmacology , Terpenes/chemistry , Terpenes/pharmacology , Cyclization , HIV-1/drug effects , Influenza A Virus, H7N1 Subtype/drug effects , Models, Molecular , Molecular Conformation , Oximes/chemistry
5.
Eur J Med Chem ; 68: 203-11, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23974020

ABSTRACT

It has been previously shown that semi-synthetic A-secotriterpene acetylhydrazones of 1-cyano-28-methoxy-28-oxo-2,3-seco-2-norlup-20(29)-en-3-al and 1-cyano-2,3-seco-2-nor-19ß,28-epoxy-18αH-olean-3-al (1, 2) inhibit the vesicular stomatitis virus (VSV) replication. To improve the antiviral activity against VSV, structural modifications of compounds 1 and 2 were performed, and new A-secoderivatives containing the acetylhydrazone fragment were obtained from betulonic acid and its methyl ester, allobetulone, and 3-oxo-18ßH-glycyrrhetinic acid methyl ester. The inhibitory effects of these compounds on VSV replication in porcine embryo kidney (PEK) cells were determined after infection. It was shown that introduction of the 3'-acetyl-5'-methyl-1',3',4'-oxadiazoline fragment into lupane triterpene structures lead to an increase in the antiviral activity of A-secotriterpene derivatives. However, the presence of a heterocyclic moiety enhanced toxic activity and reduced the therapeutic indices of these agents. Investigation in the anti-proliferative activity of the heterocyclic derivatives has shown high sensitivity of A-549, MS and RD tumor cell lines to lupane (R)-oxadiazoline 11a. The pro-apoptotic effect of 11a was confirmed by the AnnexinV/PI analysis.


Subject(s)
Antiviral Agents/chemical synthesis , Antiviral Agents/pharmacology , Hydrazones/chemical synthesis , Hydrazones/pharmacology , Vesiculovirus/drug effects , Animals , Antiviral Agents/chemistry , Cell Line, Tumor , Cells, Cultured , Crystallography, X-Ray , Flow Cytometry , Humans , Hydrazones/chemistry , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Molecular Structure , Swine , Triterpenes/chemical synthesis , Triterpenes/chemistry , Triterpenes/pharmacology , Virus Replication/drug effects
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