ABSTRACT
The amount of lipid peroxidation products (conjugated dienes and trienes and Schiff bases) in cortical neurons of the cerebral hemispheres decreased by 30% at the peak of convulsions observed 10-15 min after intraperitoneal injection of picrotoxin. In neuroglial cells of control animals the intensity of lipid peroxidation in was 1.7-2.0 times lower. Picrotoxin had no effect on this parameter.
Subject(s)
Cerebral Cortex/metabolism , Lipid Peroxidation , Seizures/metabolism , Animals , Convulsants/toxicity , Free Radicals/metabolism , Lipid Peroxidation/drug effects , Male , Neuroglia/metabolism , Neurons/metabolism , Picrotoxin/toxicity , Rats , Seizures/chemically inducedABSTRACT
The effects of the intranasal administration of preparations made from the cerebrospinal fluid of male and female opiate users on the open-field rat behavior were studied. Behavioral differences were demonstrated in the effects of preparations from female and male cerebrospinal fluid. The administration of the "male" preparation produced a significant decrease in the locomotor activity and increase in the immobilization time and grooming duration, while the "female" preparation had the opposite effects. These differences may result from different content of endogenous and exogenous opiates and dopamine (and its metabolites) in the cerebrospinal fluid of male and female opiate users.
Subject(s)
Grooming/physiology , Motor Activity/physiology , Opioid-Related Disorders/cerebrospinal fluid , Animals , Female , Humans , Male , Opioid-Related Disorders/physiopathology , Rats , Rats, Wistar , Sex FactorsABSTRACT
Cortisol administration for 3 days prior to a 6-day rest decreased formation of lipid peroxides in the rat hippocampus under stress. Exogenous cortisol limits the response of oxygen-reactive species to the lipids and exerts a regulating influence on development of a response to stress.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Hippocampus/drug effects , Hydrocortisone/administration & dosage , Lipid Peroxidation/drug effects , Stress, Physiological/metabolism , Animals , Brain Chemistry/physiology , Electric Stimulation , Hippocampus/chemistry , Hippocampus/metabolism , Injections, Intraperitoneal , Lipid Peroxidation/physiology , Lipid Peroxides/analysis , Male , Rats , Rats, Wistar , Schiff Bases/analysis , Time FactorsABSTRACT
Na+,K(+)-ATPase activity was studied in neurones and neuroglia under conditions of convulsions caused by picrotoxin administration. Picrotoxin is a stimulant which causes convulsions by suppression of presynaptic inhibition. Na+,K(+)-ATPase activity in neuroglia was increased in convulsion, bat was not altered in neurones. It is possible, that glial Na+, K(+)-ATPase activity plays the role of neuronal ion's flows regulator and neuronal protector from convulsions. In recovery period ATPase activities in neuronal and neuroglial cells run up to control.
Subject(s)
Neuroglia/enzymology , Neurons/enzymology , Seizures/enzymology , Sodium-Potassium-Exchanging ATPase/metabolism , Animals , Convulsants/toxicity , Male , Picrotoxin/toxicity , Rats , Seizures/chemically inducedABSTRACT
The study has shown seasonal changes in the responses of the frog bladder isolated preparation to exogenous adrenalin. The finding is explained by heterogeneity of the smooth muscle adrenoreceptors and alteration of functionally domineering populations during the winter-spring cycle. The changes corresponded to modifications of the fraction composition of phospholipids, the modifications being, probably, one of the ways of regulation of the smooth muscle adrenoreceptor apparatus' activity.
Subject(s)
Phospholipids/analysis , Receptors, Adrenergic/physiology , Seasons , Urinary Bladder/physiology , Adrenergic Antagonists/pharmacology , Animals , Chromatography, Thin Layer , Dose-Response Relationship, Drug , Epinephrine/pharmacology , In Vitro Techniques , Male , Rana temporaria , Receptors, Adrenergic/drug effects , Spectrophotometry, Infrared , Urinary Bladder/chemistry , Urinary Bladder/drug effectsABSTRACT
Metabolism of individual phospholipids was studied in neurones and neuroglia under conditions of convulsions caused by picrotoxin administration. Metabolic activity of all the phospholipids studied was increased in neuronal membranes under extreme conditions of convulsive attacks. The rate of metabolism of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin was increased 2-2.5-fold approximately. The most distinct alterations of metabolic activity were observed in the fraction of minor phospholipids: specific radioactivity of phosphatidylserine was increased 10-fold and phosphatidylinositol--16-fold. Metabolism of phosphatidic acid and lysophosphatidylcholine was elevated more than 15-fold. Convulsions exhibited the most distinct effect on metabolism of phosphatidylinositol and phosphatidylserine in neuroglia cells. As distinct from neurones, metabolism of phosphatidyl choline and sphingomyelin was not markedly altered in neuroglia. Similar metabolism and universal cell response, carried out via phospholipids, appear to occur in the system neurone-neuroglia.