Comparative data of childhood and adolescence molestation in heterosexual and homosexual persons.

In research with 942 nonclinical adult participants, gay men and lesbian women reported a significantly higher rate of childhood molestation than did heterosexual men and women. Forty-six percent of the homosexual men in contrast to 7% of the heterosexual men reported homosexual molestation. Twenty-two percent of lesbian women in contrast to 1% of heterosexual women reported homosexual molestation. This research is apparently the first survey that has reported substantial homosexual molestation of girls. Suggestions for future research were offered.

Psychology of the scientist: LXXXI. Professional school and traditional program graduates: comparison on measures of achievement in clinical psychology.

Clinical psychologists who graduated from traditional programs and those who graduated from professional schools were compared on both scientifically and professionally oriented criteria of achievement and recognition. Upon controlling for year of graduation from a doctoral program, the professional school graduates were less likely to be APA fellows, less likely to be on the editorial board of specified research oriented journals in clinical psychology, less likely to have diplomate status in the American Board of Professional Psychology (ABPP), less likely to have been president of state psychological associations, and less likely to have been APPIC internship directors.

Examination for professional practice in psychology subtest scores of professional and traditional clinical psychology program graduates.

Professional school clinical psychology graduates not only exhibit inferior performance on the research subtest of the Examination for Professional Practice in Psychology (EPPP; D. I. Templer & M. E. Tomeo, 1998), but they also perform less well on the four more professionally oriented subtests: diagnosis, intervention, professional/ethical/legal issues, and application to social systems. Graduates of free-standing professional schools exhibited performances inferior to those of graduates of non-free-standing professional schools on the diagnosis, intervention, and professional/ethical/legal subtests.

Mean score stability over time of programs on the clinical psychology examination for professional practice in psychology.

A correlation of .80 was obtained between mean national licensing examination score in 1988-1995 of graduates of clinical programs and mean score of these programs in 1997. It was maintained that this stability over time strengthens the inference that this mean score is a good index of program quality.

A conditional sterol esterification defect in yeast having either a sec1 or sec5 mutation in the secretory pathway.

Two temperature-conditional secretory mutations, sec1 and sec5, cause the accumulation of post-Golgi vesicles when strains containing these mutations are grown at 37 degrees C. In addition to accumulating vesicles, the mutants do not esterify free sterol on rich media at the restrictive temperature. It is the high level of inositol in the media that causes this condition in the yeast Saccharomyces cerevisiae, not a defective steryl ester synthase or lack of substrates. When strains containing the sec1 or sec5 mutation were transformed separately with a plasmid carrying SEC1 and SEC5, the esterification and secretory defects were alleviated. Double mutants containing sec6, sec14 or sec18 with either a sec1 or sec5 mutation have normal esterification levels. Strains with suppressor mutations were isolated that grew at 37 degrees C, esterified sterols and had diminished accumulation of vesicles, when grown at the restrictive temperature on defined media with additional inositol. Electron microscopy was used to examine vesicle accumulation, the number of lipid droplets, and to further characterize the esterification defect. When grown at 37 degrees C on defined medium, the strains with sec5 or sec1 accumulated the usual secretory vesicles, but when grown under similar conditions with elevated levels of inositol, accumulated an additional vesicular-like body.

Effects of unsaturated fatty acid supplementation on phospholipid and triacylglycerol biosynthesis in Saccharomyces cerevisiae.

A fatty acid desaturase mutant was used to study the regulatory effects of unsaturated fatty acids on glycerolipid biosynthesis in yeast. Cells grown on palmitoleic acid (16:1) contain approximately twice the amount of phospholipids and triacylglycerols (per mg. dry weight) compared to those grown on oleic acid (18:1). The in vitro specific activity of glycerol-3-phosphate acyl transferase was two fold higher when palmitoyl-CoA was used as a substrate relative to oleoyl-CoA. In vivo methylation studies revealed that cells grown on palmitoleic acid produce 2.6 fold more phosphatidylcholine via the CDP-DAG (methylation) pathway than cells grown on oleic acid, although oleic acid facilitated the direct phosphorylation of exogenously supplied choline. These data indicate that unsaturated fatty acids may act as key regulatory molecules which influence the glycerolipid biosynthetic matrix in yeast.

Effect of sterol alterations on conjugation in Saccharomyces cerevisiae.

Sterol auxotrophic strains of Saccharomyces cerevisiae were grown and allowed to conjugate on media supplemented with various sterols. The mating efficiency of the auxotrophs is perturbed by the replacement of the normal yeast sterol, ergosterol, with other sterols. After 4 h of mating, cells grown on ergosterol exhibited a 30-fold higher productive mating efficiency than those cells grown in stigmasterol. Aberrant budding by the conjugants was enhanced following incubation on stigmasterol and other non-ergosterol sterols. Using light and electron microscopy, we demonstrated that there is a reduced ability for stigmasterol-grown cells to undergo cytoplasmic fusion during conjugation. Many of the mated pairs remained adherent but prezygotic even after 12 h of incubation. The addition of ergosterol to cells previously grown on stigmasterol rescued the organisms, allowing for zygote formation and normal budding.