ABSTRACT
Hospitals in Shinagawa-ku and Ota-ku, the secondary medical sphere of Tokyo, have been working on various medical care tasks in order to provide a seamless transition from hospital to home medical care. In the area of nutritional medical therapy, the Shinagawa and Ota nutrition workshop was founded in 2006 to share the information, which contains thoroughly from a special nutrition therapy to a general therapy. In September 2007, a nutrition questionnaire survey was conducted for the members of Ebara-, Shinagawa-, Omori-, Kamata- and Denen-Chofu-medical association to make themes of nutrition related cooperation pass candidates. Then, we debated on the theme of priorities based on the result of the survey. In the scientific meeting held in May 2010, we examined the candidates, and decided to enrich the present diabetes passes and to a new establishment of PEG management.
Subject(s)
Community Health Services , Nutritional Support , Societies, Medical , Patient Discharge , Surveys and QuestionnairesABSTRACT
Topsoil samples were collected from eight golf courses in Yamaguchi Prefecture, Japan, and enrichment cultures were carried out with a basal-salt medium containing 0.2% 4-tert-octylphenol polyethoxylate (OPPEO) as sole carbon source. OPPEO-degrading activity was detected in one of the samples, from which a strain of OPPEO-degrading bacterium was isolated. The isolated bacterium grew on a nutritionally enriched medium (NE medium) containing 0.2% OPPEO as sole carbon source, and accumulated 4-tert-octylphenol diethoxylate (OP2EO) (63%), 4-tert-octylphenol triethoxylate (OP3EO) (14%), and 4-tert-octylphenol monoethoxylate (OP1EO) (2%) after 7 d cultivation under aerobic conditions. The addition of clay mineral (vermiculite) to the medium accelerated the degradation of OP2EO (40%) and OP3EO (4%) to OP1EO (23%). This is the first report about bacteria that can degrade OPPEO to OP1EO under aerobic conditions. The strain was identified as Sphingomonas macrogoltabidus, based on the homology of a 16S rDNA sequence.
Subject(s)
Ethyl Ethers/metabolism , Phenols/metabolism , Sphingomonas/isolation & purification , Aerobiosis , Aluminum Silicates/pharmacology , Biodegradation, Environmental/drug effects , Chromatography, High Pressure Liquid , DNA, Bacterial/genetics , Ethyl Ethers/chemistry , Gas Chromatography-Mass Spectrometry , Molecular Structure , Phenols/chemistry , Phylogeny , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , RNA, Ribosomal, 16S/genetics , Soil Microbiology , Soil Pollutants/analysis , Soil Pollutants/metabolism , Sphingomonas/genetics , Sphingomonas/metabolismABSTRACT
BACKGROUND: The whole blood rapid troponin T test, used to determine the early diagnosis of acute myocardial infarction (AMI), is effective only for 3-4 h after onset. METHODS AND RESULTS: The present office cardiologists cooperative study compared the diagnostic efficacy of a newly developed whole blood rapid panel test for heart-type fatty acid-binding protein (H-FABP) with that of the rapid troponin T test in 129 consecutive patients with suspicious AMI according to certain time-frames from onset to presentation. Thirty-one patients (24.0%) had a final diagnosis of AMI. The respective sensitivities of the rapid H-FABP and troponin T tests were 100% vs 50% (p<0.05) for patients presenting within 3 h of onset; 75% vs 0% for those between 3 and 6 h; 100% vs 60% for those between 6 and 12 h; and 100% vs 100% for those presenting later than 12 h. The respective specificities were 63% vs 96.3% (p<0.05); 93.8% vs 93.8%; 72.7% vs 100%; and 75.0% vs 87.5%. Negative predictive value was 100% vs 86.7%; 93.8% vs 78.9%; 100% vs 84.6%; and 100% vs 100%, respectively. Patients with non-AMI myocardial damage associated with unstable angina or severe heart failure showed positive H-FABP test results and blunted the specificity. CONCLUSIONS: When using the novel rapid H-FABP test, cardiac emergency triage to exclude non-AMI patients should be effectively organized within 3 h of onset.
Subject(s)
Carrier Proteins/blood , Myocardial Infarction/diagnosis , Troponin T/blood , Adult , Aged , Aged, 80 and over , Blood Chemical Analysis , Cardiology , Diagnostic Errors , Fatty Acid-Binding Proteins , Female , Humans , Male , Middle Aged , Myocardial Infarction/blood , Office Visits , Sensitivity and Specificity , Time Factors , Triage/methodsABSTRACT
The intravenous administration of beta-cryptoxanthin (320 microg/mouse) into 4-wk-old ddY mice caused a new peak in addition to the peak of beta-cryptoxanthin during high-performance liquid chromatography (HPLC) of the lipid fraction of lung homogenate. Different HPLC conditions revealed that the new peak might be attributed to a beta-carotene-like compound. The average retention times for the new peak and authentic all-trans-beta-carotene were 14.97 and 15.00 min, respectively, in a HPLC system using a YMC-Pack ODS-A column and methanol-based mobile phase, and 27.05 and 26.93 min, respectively, in a HPLC system using a Waters Nova Pack C18 column and methanol-based mobile phase. In a HPLC system using a Waters Nova Pack C18 column and acetonitrile-based mobile phase, the retention times were 10.73, 10.48 and 10.70 min for the new peak, authentic all-trans-beta-carotene, and 9-cis-beta-carotene, respectively. Spectrophotometry with a photodiode array detector showed maximum absorption of 447 and 475 nm for the new peak, and 450 and 475 nm for authentic all-trans-beta-carotene. This new peak was not observed in the lung tissue of control mice. These findings indicate the possible conversion of beta-cryptoxanthin to a beta-carotene like-compound in ddY mice.
