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Carbohydr Polym ; 275: 118681, 2022 Jan 01.
Article in English | MEDLINE | ID: mdl-34742411

ABSTRACT

Circular dichroism (CD) and small-angle X-ray scattering (SAXS) measurements were made for three xanthan samples, a double helical polysaccharide, in 5 or 10 mM aqueous NaCl after rapid temperature change to investigate the kinetics of the conformational change between the ordered and disordered states. After the rapid heating, the CD signal mainly reflecting the carbonyl groups on the side chains quickly changed (<150 s) while the scattering intensity from SAXS around q (magnitude of the scattering vector) = 1 nm-1 changed more gradually, reflecting the main-chain conformation. The difference between CD and SAXS implies us the intermediate conformation which can be regarded as a loose double helix. The SAXS profile in the rapid cooling process showed that the loose double helical structure was constructed within 150 s, but the CD signal slowly changed with around 2 days to recover the native tight double helix.


Subject(s)
Polysaccharides, Bacterial/chemistry , Sodium Chloride/chemistry , Water/chemistry , Carbohydrate Conformation , Circular Dichroism/methods , Kinetics , Molecular Conformation , Scattering, Small Angle , Temperature , X-Ray Diffraction/methods
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