[Synthesis of multiple forms of brain cholinesterases]. / Sintez mnozhestvennykh form kholinésterazy mozga.

Highly purified multiple forms of brain cholinesterase were isolated by a new method. Eight forms were identified as acetyl cholinesterases, seven forms--as butyryl cholinesterases, four forms--as mixed acetyl- and butyryl cholinesterases and four forms--as aliesterases. Biosynthesis of multiple forms of brain cholinesterase was monitored by following incorporation of 14C-glycine into the purified fractions. Cats were killed at different periods (3 hrs, 3,6 and 9 days) after intoxication with isopropyl hydroxymethyl fluorophosphate. The restoration of the enzymatic activity after the inhibition did not correspond to the incorporation of the labelled amino acid into the enzyme protein. There was a transcient increase in the incorporation of the label after an increase in acetylcholine concentration.

[Multiple forms of brain cholinesterase in phylogenesis and ontogenesis of vertebrates]. / Issledovanie mnozhestvennykh form kholinesterazy mozga v filogeneze i ontogeneze pozvonochnykh

Multiple forms of the brain cholinesterase have been investigated by means of the original technique based on the extraction, ammonium sulphate salting out and gel filtration on Sephadex G-200. It was shown that the number of multiple forms increases in phylogenesis (8 in triton, 13 in rat, 18 in cat, 16 in dog and 23 in man), although their relative enzymic activity decreases. Isolated multiple forms with high molecular weight enzymatically are classified as acetylcholinesterases. In higher brain structures, multiple forms are more numerous. Butyrylcholinesterases and aliesterases are more abundant in lower brain structures. In the neural plate of the developing triton, it is possible to detect the forms with high specific activity, which exceeds that in adult animals.