Changes in the Composition of the Soil Bacterial Community in Heavy Metal-Contaminated Farmland.

The structural changes of microorganisms in soil are the focus of soil indicators research. The purpose of this study was to investigate the changes in the composition of the soil bacterial community in heavy metal-contaminated soil. A total of six soil samples (two sampling times) were collected from contaminated farmland at three different depths (surface, middle, and deep layer). The pH value was measured. The concentrations of heavy metals (Cr, Ni, Cu, Zn, Cd, and Pb) and the soil bacterial community were analyzed using ICP-OES and 16S rRNA gene sequencing. The results of the two samplings showed that the pH value in the deep layer decreased from 6.88 to 6.23, and the concentrations of Cu, Zn, Cd, and Pb, with a smaller ion radius, increased by 16-28%, and Shannon, Chao1 increased by ~13%. The bacteria community composition at the three depths changed, but Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla. In the copper and zinc tolerance test, the isolated bacterium that was able to tolerate copper and zinc was Bacillus sp. We found that, the longer the heavy metal pollution was of concern, the higher the tolerance. These results can be used as references for the microbial remediation of heavy metal-contaminated farmland.

Effectiveness of engineering interventions in decreasing worker exposure to metalworking fluid aerosols.

Machine industry accounts for the highest proportion of primary industry in Taiwan. Long-term exposure to metalworking fluid aerosols may pose significant threats to the health of workers. The aims of this study were to verify the efficacy of intervention methods in reducing airborne bacterial concentrations as well as effects on aerosol particle size distribution. This study evaluated airborne bacterial concentrations in a large precision machinery factory in Taichung, Taiwan, before and after the implementation of intervention methods. The installation of local exhaust systems and improvement of the operation mode were used as intervention methods. Concentration and size distribution of bacteria in the metal working environment were assessed using Andersen one-stage and six-stage viable impactors, respectively. The analytical results indicate that most bacterial concentrations were less than the recommended concentrations in the indoor air quality standards (500 CFU/m3) proposed by the United States Association of Advancing Occupational and Environmental Health (US ACGIH) before the installation of local exhaust systems. There was no significant difference in bacterial concentrations before and after the installation of the local exhaust and the intervention effect on reducing bacterial exposure was not as expected. The bacterial concentrations were significantly lowered in the Z region after improving the operation mode. The particle size of bioaerosols <7.0 µm was also substantially reduced. Improper operation modes are likely to expose employees to higher concentrations of bacteria within a short time period. It recommends adjusting the metal processing methods and proper use of air spray guns for decreasing high airborne bacterial concentrations exposure.

Effects of Reductants on Phytoextraction of Chromium (VI) by Ipomoea aquatica.

Reductants are often used to reduce Cr(VI) in chemical treatments, yet the effects of the reductants on Cr(VI) phytoremediation are not fully understood. This study investigates the effects of different reductants on Cr(VI) phytoremediation by Ipomoea aquatica in simulated solution with 3 mg L(-1) of Cr(VI), pH0 of 6, and an incubation time of 5 days. Results indicate that the applications of S2O3(2-), Fe0, and Fe2+ at low doses notably increased root Cr concentrations, which were obviously higher than that those in the control (Cr6+ alone). However, high reductant concentrations decreased bioaccumulation of Cr in the roots and shoots of the plant. Statistical results indicate that Cr concentrations were significantly and negatively correlated with Fe concentrations in the roots and shoots of the plant (p<0.05). This suggest that Fe accumulation inhibited Cr accumulation in the plant. A Cr(VI) concentration of 3 mg L(-1) caused short, brown lateral roots with tip necrosis, leaf chlorosis, and noticeable shoot wilting. The leaf necrosis and shoot wilting is caused by oxidative damage of lateral roots by Cr(VI) rather than by the reactive oxygen species generated by the oxidative stress. Addition of the reductants effectively reduced these plant injuries.

Evaluation of acute toxicity and teratogenic effects of disinfectants by Daphnia magna embryo assay.

Three common disinfectants were selected in this study to investigate their toxicity to Daphnia magna. The methods used in this study included the traditional acute toxicity test, new embryo toxicity test, and teratogenic test. The study concluded that the acute toxicity of the three disinfectants to young daphnids and embryos were hypochlorite > formaldehyde > m-cresol. The effects on growth mostly occurred in the late stages of organogenesis. Of the organs, the Malpighian tube was the most sensitive to disinfectants during embryonic organogenesis. After exposure of the disinfectants to sunlight for 4 h, acute toxicity and teratogenic effects of hypochlorite on young daphnids decreased by 30% and 71%, respectively, while those of formaldehyde decreased by 35% and 49%, respectively. In addition, comparing toxic endpoints of the three disinfectants with and without sunlight exposure, the embryo tests were equally sensitive to the three-week reproduction test in this study.