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1.
Pain ; 137(1): 182-201, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18160218

ABSTRACT

Neuroinflammatory and neuroimmune mechanisms, as exemplified by infiltrating immune cells and activation of resident endothelial/glial cells, respectively, are known to be involved in the establishment and maintenance of chronic pain. An immune system pathway that may be involved in the activation of both immune and glial cells is complement. The complement pathway is made up of a large number of distinct plasma proteins which react with one another to opsonize pathogens and induce a series of inflammatory responses to help fight infection. Cleaved products and complexes produced by complement activation are responsible for a range of effects including mediation of immune infiltration, activation of phagocytes, opsonization/lysis of pathogens and injured cells, and production of vasoactive amines such as histamine and serotonin. Gene-expression microarray-analysis performed on the rat spinal nerve ligation (SNL) model of neuropathic pain revealed that multiple complement components including the C1 inhibitor, C1q alpha, beta, and gamma, C1r, C1s, C2, C3, C4, C7, and factors B, D, H, and P, were up-regulated while DAF was down-regulated. Regulation of C3 and DAF was confirmed by real-time RT-PCR and in situ hybridization. To test the hypothesis that complement plays a role in neuropathic pain, SNL rats were treated with cobra venom factor (CVF) to deplete plasma of complement component C3. Pain behavior was significantly attenuated in SNL rats treated with CVF as was complement activity at the ipsilateral dorsal root ganglia. Our results suggest the complement pathway might be a novel target for the development of neuropathic pain therapeutics.


Subject(s)
Complement Activation/physiology , Disease Models, Animal , Neuralgia/immunology , Neuralgia/physiopathology , Spinal Nerves/physiology , Animals , Complement Activation/genetics , Ligation , Neuralgia/genetics , Oligonucleotide Array Sequence Analysis/methods , Peripheral Nervous System/immunology , Peripheral Nervous System/pathology , Peripheral Nervous System/physiology , Rats , Spinal Nerves/immunology , Spinal Nerves/pathology
2.
Dev Biol ; 251(2): 348-66, 2002 Nov 15.
Article in English | MEDLINE | ID: mdl-12435363

ABSTRACT

Members of the UNC-5 protein family are transmembrane receptors for UNC-6/netrin guidance cues. To analyze the functional roles of different UNC-5 domains, we sequenced mutations in seven severe and three weak alleles of unc-5 in Caenorhabditis elegans. Four severe alleles contain nonsense mutations. Two weak alleles are truncations of the cytodomain, but one is a missense mutation in an extracellular immunoglobulin domain. To survey the function of different regions of UNC-5, wild-type and mutant unc-5::HA transgenes were tested for their ability to rescue the unc-5(e53) null mutant. Our data reveal partial functional requirements for the extracellular domains and identify a portion of the cytoplasmic juxtamembrane (JM) region as essential for rescue of migrations. When nine cytodomain tyrosines, including seven in the JM region, are mutated to phenylalanine, UNC-5 function and tyrosine phosphorylation are largely compromised. When F482 in the JM region of the mutant protein is reverted to tyrosine, UNC-5 tyrosine phosphorylation and in vivo function are largely recovered, suggesting that Y482 phosphorylation is critical to UNC-5 function in vivo. Our data also show that part of the ZU-5 motif is required for UNC-40-independent signaling of UNC-5.


Subject(s)
Caenorhabditis elegans Proteins/physiology , Cell Adhesion Molecules/physiology , Receptors, Cell Surface/physiology , Animals , Caenorhabditis elegans Proteins/analysis , Caenorhabditis elegans Proteins/chemistry , Cell Movement , Cytoplasm/chemistry , Phosphorylation , Receptors, Cell Surface/analysis , Receptors, Cell Surface/chemistry , Structure-Activity Relationship , Tyrosine/metabolism
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