ABSTRACT
OBJECTIVE: To analyze and optimize extraction technics of Polygonum orientale flowers by response surface methodology. METHODS: With the index for the content of taxifolin in flowers of Polygonum orientale, the effect of three factors such as concentration of alcohol, extraction time and solvent-solid ratio was designed by Box-Behnken central composite. Extraction technic parameters of Polygonum orientale flowers was optimized by response surface methodology. RESULTS: The optimizing extraction conditions of Polygonum orientale flowers were as follows: ethanol concentration was 65%, extracting time was 129 min and solvent-solid ratio was 18. Under the conditions, the average yield of taxifolin in 3 validation experiments was 2.79 mg/g. CONCLUSION: Optimizing extraction technics by response surface methodology is reasonable, simple, and has good predictability.
Subject(s)
Drugs, Chinese Herbal/isolation & purification , Flowers/chemistry , Plants, Medicinal/chemistry , Polygonum/chemistry , Technology, Pharmaceutical/methods , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/chemistry , Ethanol/chemistry , Linear Models , Quercetin/analogs & derivatives , Quercetin/analysis , Time FactorsABSTRACT
OBJECTIVE: To establish a HPLC-DAD method for the determination of axifolin, naringenin, quercetin and kaempferol in Cudrania tricuspidata and C. cochinchinensis in order to provide a scientific reference for species identification and quality evaluation, by establishing. METHOD: The determination was performed by HPLC-DAD on an Agilent C18 column (4.6 mm x 150 mm, 5 microm) by gradient elution (0-15 min, 35%-50% A; 15-30 min, 50% - 65% A) using methanol (A) and 0.1% phosphoric acid (B) as the mobile phase. The flow rate was 1 mL x min(-1). The detection wavelength was 290 nm for taxifolin and naringenin, 365 nm for quercetin and kaempferol with column temperature at 30 degrees C. RESULT: The content of axifolin and quercetin in the root of C. tricuspidata were remarkably higher than that in the root of C. cochinchinensis, and the content in stem of C. tricuspidata was also higher than that in the stem of C. cochinchinensis, the content of axifolin and quercetin was variable in different species. The content of naringenin and kaempferol in the root of C. cochinchinensis was visibly higher than that in the root of C. tricuspidata, and the content in the stems of the two herbs was similar, the content of naringenin and kaempferol was visibly variable in different medicinal parts of the herb, but similar between the two herbs. CONCLUSION: There's some difference of the content of the four ingredients in different medicinal parts and different herbs, so clinical use should not be confused.
Subject(s)
Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/isolation & purification , Flavones/isolation & purification , Moraceae/chemistry , Drugs, Chinese Herbal/chemistry , Flavanones/chemistry , Flavanones/isolation & purification , Flavones/chemistry , Kaempferols/chemistry , Kaempferols/isolation & purification , Methanol , Organ Specificity , Phosphoric Acids , Plant Roots/chemistry , Plant Stems/chemistry , Plants, Medicinal , Quercetin/analogs & derivatives , Quercetin/chemistry , Quercetin/isolation & purification , Reproducibility of Results , Species SpecificityABSTRACT
OBJECTIVE: To find out the correlation between the content of taxifolin in Polygonum orientale and the storage time. METHOD: HPLC was used to determine taxifolin. The chromatographic condition was as following: Diamonsil C18 column (4.6 mm x 200 mm, 5 microm), mobile phase acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength 290 nm and flow rate 1.0 mL x min(-1), the column temperature 30 degrees C. RESULT: The injection volume of taxifolin was in good linearity within 0.07 and 0.35 microg, the average recovery was 99.7% with RSD 0.2%. Taxifolin content was 0.84, 1.36, 1.75, 1.99 mg x g(-1) corresponding to storage time of 10, 7, 6, 5 years, respectively. CONCLUSION: The content of taxifolin decreased with the storage time. When the storage period is more than six years, the content is lower than that required by Chinese Pharmacopoeia (2010 version). This method has a good repeatability and accuracy, it provides a scientific reference for clinical use and quality evaluation of P. orientale.
Subject(s)
Drug Storage/methods , Drugs, Chinese Herbal/analysis , Polygonum/chemistry , Quercetin/analogs & derivatives , Chromatography, High Pressure Liquid , Drug Stability , Quercetin/analysisABSTRACT
OBJECTIVE: To establish a characteristic HPLC fingerprint of Polygonum orientale inflorescence, and to provide reference for its quality evaluation. METHODS: Taxifolin was used as reference. HPLC analysis was carried out with Diamonsil C18 column (200 mm x 4.6 mm, 5 microm) using acetonitrile -0.1% phosphoric acid(gradient elution)as mobile phase at flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm and the column temperature was 30 degrees C. RESULTS: Eighteen common peaks were pointed out from the HPLC fingerprint of Polygonum orientale inflorescence from 12 different habitats. Among of them,four common peaks were identified as taxifolin, catechin, gallic acid and 3,3'-dimethyl ellagic acid-4-O-beta-D-glucoside. Analyzed by "Similarity Evaluation for Chromatographic Fingerprint of Traditional Chinese Medicine" software, the HPLC fingerprint similarities of 12 samples were more than 0.9. CONCLUSION: This method is repeatable and exclusive. It can be used for identification and quality control of Polygonum orientale inflorescence.
