ABSTRACT
Periodontal ligament (PDL) cells are fibroblasts that play key roles in tissue integrity, periodontal inflammation and tissue regeneration in the periodontium. The periodontal tissue destruction in periodontitis is mediated by host tissue-produced inflammatory cytokines, including interleukin-1ß (IL-1ß). Here, we report the expression of G protein-coupled receptor 30 (GPR30, also known as G protein-coupled estrogen receptor 1 GPER) in human PDL cells and its regulation by IL-1ß. IL-1ß-induced GPR30 expression in human PDL cells leads to the activation of multiple signaling pathways, including MAPK, NF-κB and PI3K. In contrast, genistein, an estrogen receptor ligand, postpones the activation of MAPKs induced by IL-1ß. Moreover, the inhibition of GPR30 by G15, a GPR30-specific antagonist, eliminates this delay. Thus, genistein plays a role in the regulation of MAPK activation via GPR30, and GPR30 represents a novel target regulated by steroid hormones in PDL cells.
Subject(s)
Genistein/pharmacology , Interleukin-1beta/physiology , Mitogen-Activated Protein Kinases/metabolism , Periodontal Ligament/drug effects , Receptors, Estrogen/physiology , Receptors, G-Protein-Coupled/physiology , Adolescent , Base Sequence , Cells, Cultured , DNA Primers , Enzyme Activation , Female , Humans , Male , Periodontal Ligament/cytology , Periodontal Ligament/enzymology , Real-Time Polymerase Chain Reaction , Receptors, Estrogen/antagonists & inhibitors , Receptors, G-Protein-Coupled/antagonists & inhibitorsABSTRACT
PURPOSE: The aim of this prospective randomized controlled trial is to evaluate the clinical effect of glass fiber-reinforced post core applied in the restoration of premolar or molar residual crown and root. METHODS: 195 residual crown and root of posterior teeth from 100 patients, aged from 18 to 60 years, were included in the study. Among 195 teeth, 95 teeth in the experimental group were restored with glass fiber-reinforced post core, while the other 100 teeth in the control group were restored with metal cast post core. All the teeth were then restored with porcelain fused metal crown. The esthetical property, function, and survival rate of the prostheses were observed. The data was analyzed with SPSS13.0 software package for X(2) test. RESULTS: The prostheses were followed up in this clinical study for a period of up to 36 months. In the experimental group, there were a total of 90 teeth with excellent chewing function, no complaint of discomfort, no debounding, no gingivitis or periapical periodontitis. The success rate of the experimental group was 94.74%, while that of the control group was 85.00%. The difference of the success rate between two groups was statistically significant(P<0.05). CONCLUSION: Glass fiber-reinforced post core can be used as an ideal restoration material for the premolar or molar residual crown and root.
Subject(s)
Crowns , Post and Core Technique , Adolescent , Adult , Bicuspid , Dental Porcelain , Glass , Humans , Middle Aged , Prospective Studies , Tooth Crown , Young AdultABSTRACT
PURPOSE: The purpose of this study is to evaluate immediate autologous bone graft combined with secondary dental implant to repair traumatogenic defect and lost teeth. METHODS: 16 patients with labial anterior maxillary alveolar bone defect, caused by trauma for various reasons were selected as the experimental group. The suitable size autologous skull plates or chin bone plates were harvested and transplanted to the bone defect site and fixed with titanium screw immediately after the wound was completely cleaned, and then the gingiva and mucosa were sutured adequately. 42 ITI SLA implants were implanted 12 weeks after bone graft surgery. Porcelain fused metal crowns were made 12 weeks after implantation. Meanwhile, a total of 22 patients with 50 ITI SLA implants which were routinely implanted in anterior maxillary alveolar bone without bone graft surgery were selected as the control group. Resonance frequency analysis(RFA) was taken at 0,4,12,52 weeks after implant surgery in both groups to get implant stability quotient(ISQ) values. Student's t test was used to calculate P values for ISQ with SPSS13.0 software package. RESULTS: The soft tissue wound of 16 patients healed 7-9 days after trauma. The alveolar arch returned normal, the alveolar ridge was with adequate height and width. The contour and function of dentures restored 12 weeks after implantation. No significant difference in ISQ values was found between the two groups at 0,4,12,52 weeks after implant surgery(P>0.05). CONCLUSIONS: The course of treatment is shortened, the times of surgery are reduced, and the repair effect is satisfied by immediate autologous bone graft and secondary dental implantation for upper anterior labial alveolar bone defect and teeth loss caused by trauma.
Subject(s)
Bone Transplantation , Dental Implantation, Endosseous , Dental Prosthesis, Implant-Supported , Alveolar Bone Loss , Dental Implantation , Dental Implants , Dental Restoration Failure , Humans , MaxillaABSTRACT
Two minipeptides (BmP02 and BmP03) with M.W. 2.950 kD and 2.935 kD determined respectively by electrospray ionization mass spectrum, were purified from the venom of Buthus martensi Karsch by an opportune procedure on cation-exchange column chromatography and repeated HPLC. The amino acid sequence of BmP02 had been determined. Using whole-cell patch clamp, BmP02 was studied on rat ventricular myocyte. The results showed that BmP02 inhibited the transient outward current (Ito) of rat ventricular myocytes with partial recovery after washout (n=5) and its inhibition had concentration dependence and voltage independence. Further study showed that BmP02 affected both the inactivation and recovery kinetics, but not activation kinetics of Ito channel.
