Enhancement of the Mechanical Properties of Hydrogels with Continuous Fibrous Reinforcement.

Reinforcing mechanically weak hydrogels with fibers is a promising route to obtain strong and tough materials for biomedical applications while retaining a favorable cell environment. The resulting hierarchical structure recreates structural elements of natural tissues such as articular cartilage, with fiber diameters ranging from the nano- to microscale. Through control of properties such as the fiber diameter, orientation, and porosity, it is possible to design materials which display the nonlinear, synergistic mechanical behavior observed in natural tissues. In order to fully exploit these advantages, it is necessary to understand the structure-property relationships in fiber-reinforced hydrogels. However, there are currently limited models which capture their complex mechanical properties. The majority of reported fiber-reinforced hydrogels contain fibers obtained by electrospinning, which allows for limited spatial control over the fiber scaffold and limits the scope for systematic mechanical testing studies. Nevertheless, new manufacturing techniques such as melt electrowriting and bioprinting have emerged, which allow for increased control over fiber deposition and the potential for future investigations on the effect of specific structural features on mechanical properties. In this review, we therefore explore the mechanics of fiber-reinforced hydrogels, and the evolution of their design and manufacture from replicating specific features of biological tissues to more complex structures, by taking advantage of design principles from both tough hydrogels and fiber-reinforced composites. By highlighting the overlap between these fields, it is possible to identify the remaining challenges and opportunities for the development of effective biomedical devices.

Micromechanical Modeling Study of Mechanical Inhibition of Enzymatic Degradation of Collagen Tissues.

This study investigates how the collagen fiber structure influences the enzymatic degradation of collagen tissues. We developed a micromechanical model of a fibrous collagen tissue undergoing enzymatic degradation based on two central hypotheses. The collagen fibers are crimped in the undeformed configuration. Enzymatic degradation is an energy activated process and the activation energy is increased by the axial strain energy density of the fiber. We determined the intrinsic degradation rate and characteristic energy for mechanical inhibition from fibril-level degradation experiments and applied the parameters to predict the effect of the crimped fiber structure and fiber properties on the degradation of bovine cornea and pericardium tissues under controlled tension. We then applied the model to examine the effect of the tissue stress state on the rate of tissue degradation and the anisotropic fiber structures that developed from enzymatic degradation.

Minimal preconditioning effects observed for inflation tests of planar tissues.

The purpose of this study is to investigate the effects of preconditioning on the deformation response of planar tissues measured by inflation tests. The inflation response of test specimens, including the bovine cornea, bovine and porcine sclera, and human skin, exhibited a negligible evolving deformation response when subjected to repeated pressure loading with recovery periods between cycles. Tissues obtained complete recovery to the reference state, and strain contours across the entire specimen were nearly identical at the maximum pressure of each load cycle. This repeatability was obtained regardless of strain history. These results suggest that negligible permanent change was induced in the microstructure by inflation testing. Additionally, we present data illustrating that a lack of a recovery period can result in an evolving deformation response to repeated loading that is commonly attributed to preconditioning. These results suggest that the commonly observed effects of preconditioning may be avoided by experimental design for planar tissues characterized by long collagen fibers arranged in the plane of the tissue. Specifically, if the test is designed to fully fix the specimen boundary during loading, adequate recovery periods are allowed after each load cycle, and loads are limited to avoid damage, preconditioning effects may be avoided for planar tissues.

Full-field bulge test for planar anisotropic tissues: part I--experimental methods applied to human skin tissue.

The nonlinear anisotropic properties of human skin tissue were investigated using bulge testing. Full-field displacement data were obtained during testing of human skin tissues procured from the lower back of post-mortem human subjects using 3-D digital image correlation. To measure anisotropy, the dominant fiber direction of the tissue was determined from the deformed geometry of the specimen. Local strains and stress resultants were calculated along both the dominant fiber direction and the perpendicular direction. Variation in anisotropy and stiffness was observed between specimens. The use of stress resultants rather than the membrane stress approximation accounted for bending effects, which are significant for a thick nonlinear tissue. Of the six specimens tested, it was observed that specimens from older donors exhibited a stiffer and more isotropic response than those from younger donors. It was seen that the mechanical response of the tissue was negligibly impacted by preconditioning or the ambient humidity. The methods presented in this work for skin tissue are sufficiently general to be applied to other planar tissues, such as pericardium, gastrointestinal tissue, and fetal membranes. The stress resultant-stretch relations will be used in a companion paper to obtain material parameters for a nonlinear anisotropic hyperelastic model.

Full-field bulge test for planar anisotropic tissues: part II--a thin shell method for determining material parameters and comparison of two distributed fiber modeling approaches.

A thin shell method is presented to analyze the results of the bulge test presented in Part I of this paper. The method accounts for the effects of bending, which can be significant for thick tissues inflated from a planar state. We fit two commonly used hyperelastic distributed fiber constitutive models to the stretch-stress resultant data for human skin tissue calculated in Part I from the measured inflation pressure and deformed geometry of the tissue. To validate the method, the resulting parameters were implemented in a specimen-specific finite-element analysis. The method was capable of reproducing the experimentally measured pressure-stretch response of the tissue for a fully integrated distributed fiber model, but not for the pre-integrated distributed fiber models. The parameters obtained for the pre-integrated models significantly underestimated the anisotropic properties of the tissue. The thin shell method presented in this work has been applied to human skin tissues but is sufficiently general to be applied to analyze the inflation response of other planar tissues.