Gamma-linolenic acid production of Mucor rouxii by solid-state fermentation using agricultural by-products.

AIMS: This study aims to maximize the yield of gamma-linolenic acid by a filamentous fungus, Mucor rouxii, using low cost production by solid-state fermentation. METHODS AND RESULTS: We optimized substrate types and culture conditions including inoculum size and temperature. The optimal growth of M. rouxii was found in the cultures inoculated with 5 x 10(5) spores g(-1) substrate. The fungal cells grew well on rice bran and soy bean meal, whereas a lower biomass was found in the cultures grown on polished rice, broken rice and spent malt grain. The GLA content was highly accumulated in rice bran ferment and its maximal content of about 6 g kg(-1) fermented mass was observed in the 5th-day culture grown at 30 degrees C. However, the GLA content in the rice bran ferment was not enhanced by low temperature. CONCLUSIONS: The GLA production of M. rouxii could be enhanced by optimizing the agricultural by-product substrates and culture condition. SIGNIFICANCE AND IMPACT OF THE STUDY: Low cost GLA production process was achieved, and fermented product containing GLA can be incorporated into feed additives without further oil extraction to alternate the expensive plant oils.

The potential for establishment of axial temperature profiles during solid-state fermentation in rotating drum bioreactors.

The mixing and heat transfer phenomena within rotating drum bioreactors (RDBs) used for solid-state fermentation processes are poorly studied. The potential for the establishment of axial temperature gradients within the substrate bed was explored using a heat transfer model. For growth of Aspergillus oryzae on wheat bran within a 24 L RDB with air at a superficial velocity of 0.0023 m s(-1) and 15% relative humidity, the model predicts an axial gradient between the air inlet and outlet of 2 degrees C during rapid growth, compared to experimental axial temperature gradients of between 1 and 4 degrees C. Undesirably high temperatures occur throughout the bed under these operating conditions, but the model predicts that good temperature control can be achieved using humid air (90% relative humidity) at superficial velocities of 1 m s(-1) for a 204 L RDB. For a 2200 L RDB, good temperature control is predicted with superficial velocities as low as 0.4 m s(-1) with the airflow being switched from 90% to 15% relative humidity whenever the temperature at the outlet end of the drum exceeds the optimal temperature for growth. This work suggests that significant axial temperature gradients can arise in those RDBs that lack provision for axial mixing. It is therefore advisable to use angled lifters within RDBs to promote axial mixing.